The authors leveraged single-cell RNA sequencing in combination with in vivo lineage tracing and organoid models to finely map the trajectories of alveolar-lineage cells during injury repair and lung regeneration.
[Cell Stem Cell]
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Scientists propose a mechanistic theory of epithelial shells which resemble small-organoid morphologies. Using a 3D surface tension-based vertex model, they reproduced the characteristic shapes from branched and budded to invaginated structures.
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Intestinal exposure to inositol trisphosphate and phytate ingestion both promoted recovery following intestinal damage. Remarkably, inositol trisphosphate also induced growth of patient-derived intestinal organoids, stimulated HDAC3-dependent proliferation, and countered butyrate inhibition of colonic growth.
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The authors summarize recent approaches, progresses and challenges in resolving brain organoid’s complexity through single-cell transcriptomics.
[Seminars in Cell & Developmental Biology]
The authors report the directed self-organization of human induced pluripotent stem cells in a collagen hydrogel towards a highly interconnected neuronal network at a macroscale tissue format.
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Zafeiriou, M.-P., Bao, G., Hudson, J., Halder, R., Blenkle, A., Schreiber, M.-K., Fischer, A., Schild, D., & Zimmermann, W.-H. (2020). Developmental GABA polarity switch and neuronal plasticity in Bioengineered Neuronal Organoids. Nature Communications, 11(1), 3791. https://doi.org/10.1038/s41467-020-17521-w Cite
Researchers presented a simple and minimally invasive fine-needle aspiration-based organoid culture technique using a variety of tumor types including gastrointestinal, thyroid, melanoma, and kidney.
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Scientists developed expandable ovarian cancer organoids in less than three weeks; these organoids captured the characteristics of histological cancer subtypes and replicated the mutational landscape of the primary tumors.
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The authors report a method to generate ureteric bud organoids that possess epithelial polarity and tubular lumen and repeat branching morphogenesis.
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The effect of miR-3622a-3p on proliferation, apoptosis, cell cycle, migration and invasion of colorectal cancer (CRC) cells were investigated by a series of biological function assays and the results revealed that miR-3622a-3p could inhibit the malignant biological properties of CRC.
[Cell Death & Disease]
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Scientists established 12 patient-derived organoids from the most common lung cancer subtype, lung adenocarcinoma (LADC). Extensive gene and histopathology profiling show that the tumor organoids retain the histological architectures, genomic landscapes, and gene expression profiles of their parental tumors.
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Estrogen receptor positive breast cancer organoids and aromatase inhibitor (AI)- or fulvestrant-resistant breast cancer cells were more sensitive to anastrozole plus estradiol than to AI alone.
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Cairns, J., Ingle, J. N., Dudenkov, T. T. M., Kalari, K. R., Carlson, E. E., Na, J., Buzdar, A. U., Robson, M. E., Ellis, M. J., Goss, P. E., Shepherd, L. E., Goodnature, B., Goetz, M. P., Weinshilboum, R. M., Li, H., Bari, M. G., & Wang, L. (2020). Pharmacogenomics of aromatase inhibitors in postmenopausal breast cancer and additional mechanisms of anastrozole action. JCI Insight. https://doi.org/10.1172/jci.insight.137571 Cite
The French government this week unveiled a draft science bill that promises to increase public research spending with an extra €25 billion over the next ten years.