E-Cigarettes Compromise the Gut Barrier and Trigger Inflammation

Using murine models of acute and chronic e-cigarette aerosol inhalation, murine colon transcriptomics and murine and human gut-derived organoids in co-culture models, researchers assessed the effects of e-cigarette use on the gut barrier.
[iScience]
E-cigarettes compromise the gut barrier and trigger inflammation: iScience. (n.d.). Retrieved January 11, 2021, from https://www.cell.com/iscience/fulltext/S2589-0042(21)00003-1 Cite
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CREPT Is Required for Murine Stem Cell Maintenance during Intestinal Regeneration

Scientists report that CREPT, a recently identified tumor-promoting protein, was required for the maintenance of murine intestinal stem cells. CREPT was preferably expressed in the crypts but not in the villi.
[Nature Communications]
Yang, L., Yang, H., Chu, Y., Song, Y., Ding, L., Zhu, B., Zhai, W., Wang, X., Kuang, Y., Ren, F., Jia, B., Wu, W., Ye, X., Wang, Y., & Chang, Z. (2021). CREPT is required for murine stem cell maintenance during intestinal regeneration. Nature Communications, 12(1), 270. https://doi.org/10.1038/s41467-020-20636-9 Cite
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A Biomimetic Assay Platform for the Interrogation of Antigen-Dependent Anti-Tumor T-Cell Function

Researchers present an innovative ultra-high-content assay platform for interrogating T-cell-mediated killing of 3D multicellular tumor spheroids.
[Communications Biology]
To, J., Quackenbush, D., Rowell, E., Li, L., Reed, C., Lo, F., & Horman, S. R. (2021). A biomimetic assay platform for the interrogation of antigen-dependent anti-tumor T-cell function. Communications Biology, 4(1), 1–14. https://doi.org/10.1038/s42003-020-01565-1 Cite
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Ethanol Exposure Drives Colon Location Specific Cell Composition Changes in a Normal Colon Crypt 3D Organoid Model

Scientists employed RNA-sequencing to asses transcriptomic response to ethanol exposure in 3D organoid lines derived from healthy colon. Paired regression analysis identified 2,162 differentially expressed genes in response to ethanol.
[Scientific Reports]
Devall, M., Plummer, S. J., Bryant, J., Jennelle, L. T., Eaton, S., Dampier, C. H., Huyghe, J. R., Peters, U., Powell, S. M., & Casey, G. (2021). Ethanol exposure drives colon location specific cell composition changes in a normal colon crypt 3D organoid model. Scientific Reports, 11(1), 432. https://doi.org/10.1038/s41598-020-80240-1 Cite
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Engineering Human Hepato-Biliary-Pancreatic Organoids from Pluripotent Stem Cells

Investigators describe a protocol for the continuous patterning of hepatic, biliary and pancreatic structures from a 3D culture of human pluripotent stem cells.
[Nature Protocols]
Koike, H., Iwasawa, K., Ouchi, R., Maezawa, M., Kimura, M., Kodaka, A., Nishii, S., Thompson, W. L., & Takebe, T. (2021). Engineering human hepato-biliary-pancreatic organoids from pluripotent stem cells. Nature Protocols, 1–18. https://doi.org/10.1038/s41596-020-00441-w Cite
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Reprogramming Immunosuppressive Myeloid Cells by Activated T Cells Promotes the Response to Anti-PD-1 Therapy in Colorectal Cancer

The levels of infiltrating myeloid-derived suppressor cells (MDSCs) were significantly higher in the non-responding organoids and were selectively reduced in the responding group, with MDSCs showing increased apoptosis and attenuated functional activity after anti-PD-1 treatment.
[Signal Transduction and Targeted Therapy]
Chen, J., Sun, H.-W., Yang, Y.-Y., Chen, H.-T., Yu, X.-J., Wu, W.-C., Xu, Y.-T., Jin, L.-L., Wu, X.-J., Xu, J., & Zheng, L. (2021). Reprogramming immunosuppressive myeloid cells by activated T cells promotes the response to anti-PD-1 therapy in colorectal cancer. Signal Transduction and Targeted Therapy, 6(1), 1–14. https://doi.org/10.1038/s41392-020-00377-3 Cite
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Mount Sinai and Rumi Scientific Team Up to Advance Drug Discovery for Rare Genetic Disorders Tied to Autism

The Seaver Autism Center for Research and Treatment at the Icahn School of Medicine at Mount Sinai and Rumi Scientific announced today that they will team up to initiate a drug discovery pipeline for rare genetic disorders that carry a high risk of autism. The project will initially involve the development and use of a platform that mimics the development of brain tissues, at high speed, allowing researchers to gain insights into how cells respond to three known genes implicated in autism.
[Mount Sinai Health System]
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Establishment of Intestinal Organoid Cultures Modeling Injury-Associated Epithelial Regeneration

Researchers established a novel intestinal organoid culture system composed of 8 components, mainly including VPA, EPZ6438, LDN193189, and R-Spondin 1 conditioned medium, which mimics the gut epithelium regeneration that produces hyperplastic crypts following injury.
[Cell Research]
Qu, M., Xiong, L., Lyu, Y., Zhang, X., Shen, J., Guan, J., Chai, P., Lin, Z., Nie, B., Li, C., Xu, J., & Deng, H. (2021). Establishment of intestinal organoid cultures modeling injury-associated epithelial regeneration. Cell Research, 1–13. https://doi.org/10.1038/s41422-020-00453-x Cite
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Mass Generation, Neuron Labeling, and 3D Imaging of Minibrains

Scientists present a step-by-step methodology to generate human minibrain nurseries and novel strategies to subsequently label projection neurons, perform immunohistochemistry and 3D imaging of the minibrains at large multiplexable scales.
[Frontiers in Bioengineering and Biotechnology]
Govindan, S., Batti, L., Osterop, S. F., Stoppini, L., & Roux, A. (2021). Mass Generation, Neuron Labeling, and 3D Imaging of Minibrains. Frontiers in Bioengineering and Biotechnology, 8. https://doi.org/10.3389/fbioe.2020.582650 Cite
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NRL -/- Gene Edited Human Embryonic Stem Cells Generate Rod-Deficient Retinal Organoids Enriched in S-Cone-Like Photoreceptors

Researchers used CRISPR/Cas9 gene editing to engineer an NRL-deficient embryonic stem cell line, and differentiated it into retinal organoids.
[Stem Cells]
NRL−/− gene edited human embryonic stem cells generate rod‐deficient retinal organoids enriched in S‐cone‐like photoreceptors - Cuevas - - STEM CELLS - Wiley Online Library. (n.d.). Retrieved January 8, 2021, from https://stemcellsjournals.onlinelibrary.wiley.com/doi/abs/10.1002/stem.3325?af=R Cite
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Intestinal Differentiation Involves Cleavage of Histone H3 N-Terminal Tails by Multiple Proteases

Combining biochemical methods, 3D organoid cultures and in vivo approaches, scientists demonstrated that intestinal H3 clipping was the result of multiple proteolytic activities.
[Nucleic Acids Research]
Intestinal differentiation involves cleavage of histone H3 N-terminal tails by multiple proteases | Nucleic Acids Research | Oxford Academic. (n.d.). Retrieved January 7, 2021, from https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa1228/6062769 Cite
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