Using murine models of acute and chronic e-cigarette aerosol inhalation, murine colon transcriptomics and murine and human gut-derived organoids in co-culture models, researchers assessed the effects of e-cigarette use on the gut barrier.
6445218 855ANIE3 items 1 apa default asc 1
Scientists report that CREPT, a recently identified tumor-promoting protein, was required for the maintenance of murine intestinal stem cells. CREPT was preferably expressed in the crypts but not in the villi.
6445218 I9L3PTQJ items 1 apa default asc 1
Yang, L., Yang, H., Chu, Y., Song, Y., Ding, L., Zhu, B., Zhai, W., Wang, X., Kuang, Y., Ren, F., Jia, B., Wu, W., Ye, X., Wang, Y., & Chang, Z. (2021). CREPT is required for murine stem cell maintenance during intestinal regeneration. Nature Communications, 12(1), 270. https://doi.org/10.1038/s41467-020-20636-9 Cite
Researchers present an innovative ultra-high-content assay platform for interrogating T-cell-mediated killing of 3D multicellular tumor spheroids.
6445218 9JFBHRT5 items 1 apa default asc 1
Scientists employed RNA-sequencing to asses transcriptomic response to ethanol exposure in 3D organoid lines derived from healthy colon. Paired regression analysis identified 2,162 differentially expressed genes in response to ethanol.
6445218 GADIA6KA items 1 apa default asc 1
Devall, M., Plummer, S. J., Bryant, J., Jennelle, L. T., Eaton, S., Dampier, C. H., Huyghe, J. R., Peters, U., Powell, S. M., & Casey, G. (2021). Ethanol exposure drives colon location specific cell composition changes in a normal colon crypt 3D organoid model. Scientific Reports, 11(1), 432. https://doi.org/10.1038/s41598-020-80240-1 Cite
Investigators describe a protocol for the continuous patterning of hepatic, biliary and pancreatic structures from a 3D culture of human pluripotent stem cells.
6445212 88IVFDIG items 1 apa default asc 1
The levels of infiltrating myeloid-derived suppressor cells (MDSCs) were significantly higher in the non-responding organoids and were selectively reduced in the responding group, with MDSCs showing increased apoptosis and attenuated functional activity after anti-PD-1 treatment.
[Signal Transduction and Targeted Therapy]
6445218 85E8QVW4 items 1 apa default asc 1
Chen, J., Sun, H.-W., Yang, Y.-Y., Chen, H.-T., Yu, X.-J., Wu, W.-C., Xu, Y.-T., Jin, L.-L., Wu, X.-J., Xu, J., & Zheng, L. (2021). Reprogramming immunosuppressive myeloid cells by activated T cells promotes the response to anti-PD-1 therapy in colorectal cancer. Signal Transduction and Targeted Therapy, 6(1), 1–14. https://doi.org/10.1038/s41392-020-00377-3 Cite
The Seaver Autism Center for Research and Treatment at the Icahn School of Medicine at Mount Sinai and Rumi Scientific announced today that they will team up to initiate a drug discovery pipeline for rare genetic disorders that carry a high risk of autism. The project will initially involve the development and use of a platform that mimics the development of brain tissues, at high speed, allowing researchers to gain insights into how cells respond to three known genes implicated in autism.
[Mount Sinai Health System]
6445218 nan items 1 apa default asc 1
Researchers established a novel intestinal organoid culture system composed of 8 components, mainly including VPA, EPZ6438, LDN193189, and R-Spondin 1 conditioned medium, which mimics the gut epithelium regeneration that produces hyperplastic crypts following injury.
6445218 VCB85MIF items 1 apa default asc 1
Scientists present a step-by-step methodology to generate human minibrain nurseries and novel strategies to subsequently label projection neurons, perform immunohistochemistry and 3D imaging of the minibrains at large multiplexable scales.
[Frontiers in Bioengineering and Biotechnology]
Researchers used CRISPR/Cas9 gene editing to engineer an NRL-deficient embryonic stem cell line, and differentiated it into retinal organoids.
6445218 49CF66V9 items 1 apa default asc 1
NRL−/− gene edited human embryonic stem cells generate rod‐deficient retinal organoids enriched in S‐cone‐like photoreceptors - Cuevas - - STEM CELLS - Wiley Online Library. (n.d.). Retrieved January 8, 2021, from https://stemcellsjournals.onlinelibrary.wiley.com/doi/abs/10.1002/stem.3325?af=R Cite
Combining biochemical methods, 3D organoid cultures and in vivo approaches, scientists demonstrated that intestinal H3 clipping was the result of multiple proteolytic activities.
[Nucleic Acids Research]
6445218 QIW9PFVC items 1 apa default asc 1
Intestinal differentiation involves cleavage of histone H3 N-terminal tails by multiple proteases | Nucleic Acids Research | Oxford Academic. (n.d.). Retrieved January 7, 2021, from https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkaa1228/6062769 Cite