Keep Current with the Latest in Cell Biology Research
The authors demonstrated that respiratory viral infection induces distinct fibroblast activation states, which they term extracellular matrix-synthesizing, damage-responsive and interferon-responsive states. They provide evidence that excess activity of damage-responsive lung fibroblasts drives lethal immunopathology during severe influenza virus infection.
1254621 U4QJLW95 items 1 apa default asc 1
Boyd, D. F., Allen, E. K., Randolph, A. G., Guo, X. J., Weng, Y., Sanders, C. J., Bajracharya, R., Lee, N. K., Guy, C. S., Vogel, P., Guan, W., Li, Y., Liu, X., Novak, T., Newhams, M. M., Fabrizio, T. P., Wohlgemuth, N., Mourani, P. M., Wight, T. N., … Thomas, P. G. (2020). Exuberant fibroblast activity compromises lung function via ADAMTS4. Nature, 1–6. https://doi.org/10.1038/s41586-020-2877-5 Cite
Scientists incorporated the protein information to analyze the expression of ACE2, the SARS-CoV-2 receptor, together with co-factors, TMPRSS2 and Furin, at single cell level in situ, which they called protein-proofed single-cell RNA (pscRNA) profiling.
1254621 9NIDPKPL items 1 apa default asc 1
Scientists showed that inactivating mutations in the epigenetic regulator TET2, which led to clonal hematopoiesis, aggravate age- and obesity-related insulin resistance in mice.
1254621 RNRGYHCP items 1 apa default asc 1
Fuster, J. J., Zuriaga, M. A., Zorita, V., MacLauchlan, S., Polackal, M. N., Viana-Huete, V., Ferrer-Pérez, A., Matesanz, N., Herrero-Cervera, A., Sano, S., Cooper, M. A., González-Navarro, H., & Walsh, K. (2020). TET2-Loss-of-Function-Driven Clonal Hematopoiesis Exacerbates Experimental Insulin Resistance in Aging and Obesity. Cell Reports, 33(4). https://doi.org/10.1016/j.celrep.2020.108326 Cite
To delineate the clonal framework of myeloid malignancies, scientists performed single-cell mutational profiling on 146 samples from 123 patients. They showed that acute myeloid leukemia is dominated by a small number of clones, which frequently harbor co-occurring mutations in epigenetic regulators.
1254621 8U67NILM items 1 apa default asc 1
Miles, L. A., Bowman, R. L., Merlinsky, T. R., Csete, I. S., Ooi, A. T., Durruthy-Durruthy, R., Bowman, M., Famulare, C., Patel, M. A., Mendez, P., Ainali, C., Demaree, B., Delley, C. L., Abate, A. R., Manivannan, M., Sahu, S., Goldberg, A. D., Bolton, K. L., Zehir, A., … Levine, R. L. (2020). Single-cell mutation analysis of clonal evolution in myeloid malignancies. Nature, 1–6. https://doi.org/10.1038/s41586-020-2864-x Cite
The authors established a standardized culture platform using xeno- and serum-free commercial media for expansion of MSCs derived from umbilical cord, bone marrow and adipose-derived and examined their functional characteristics.
6445212 VTXVVDAS items 1 apa default asc 1
Hoang, V. T., Trinh, Q.-M., Phuong, D. T. M., Bui, H. T. H., Hang, L. M., Ngan, N. T. H., Anh, N. T. T., Nhi, P. Y., Nhung, T. T. H., Lien, H. T., Nguyen, T. D., Thanh, L. N., & Hoang, D. M. (2020). Standardized xeno- and serum-free culture platform enables large-scale expansion of high-quality mesenchymal stem/stromal cells from perinatal and adult tissue sources. Cytotherapy, 0(0). https://doi.org/10.1016/j.jcyt.2020.09.004 Cite
Using time-lapse imaging of mouse mammary tumor organoids in 3D culture, researchers observed that outgrowth potential varied non-linearly with initial organoid size.
[Journal of Mammary Gland Biology and Neoplasia]
Perfusion of tissue-engineered blood vessels at a physiological shear stress with enzyme-modified low-density-lipoprotein with or without TNFα promotes monocyte accumulation, reduced vasoactivity, alters NO production, which led to endothelial cell activation, monocyte accumulation, foam cell formation and expression of pro-inflammatory cytokines.
6445212 3CE346T6 items 1 apa default asc 1
Scientists investigated the proliferation of umbilical cord blood–derived endothelial progenitor cells and the differentiation efficiency toward corneal endothelial cell-like cells induced by rho-associated protein kinase inhibitor Y-27632 and to determine the most effective strategy for repairing corneal endothelium injuries in rabbits.
6445212 HWDZBX6C items 1 apa default asc 1
Investigators extracted collagen from human amniotic membrane and umbilical cord, which are treated as medical waste and compared its physico-chemical properties.
[International Journal of Biological Macromolecules]
6445212 YX82D7 items 1 apa default asc 1
After C–C motif chemokine ligand 2 (CCL2)-overexpressing human umbilical cord-derived MSCs were intravenously transplanted with mannitol in rats with middle cerebral arterial occlusion, scientists compared the differences between four different treatment groups: mannitol + CCL2-overexpressing hUC-MSCs, mannitol + naïve hUC-MSCs, mannitol only, and control.
[International Journal of Molecular Sciences]
6445212 Z9967ETB items 1 apa default asc 1
To establish the most robust and efficient way to measure the Treg content of previously cryopreserved cord blood units, researchers compared the enumeration of Treg and CD3+ cells using flow cytometry and an epigenetic, DNA-based methodology.
6445212 RT9BWGCT items 1 apa default asc 1
Duggleby, R. C., Tsang, H. P., Strange, K., McWhinnie, A., Lamikanra, A. A., Roberts, D. J., Hernandez, D., Madrigal, J. A., & Danby, R. D. (2020). Enumerating regulatory T cells in cryopreserved umbilical cord blood samples using FOXP3 methylation specific quantitative PCR. PLOS ONE, 15(10), e0240190. https://doi.org/10.1371/journal.pone.0240190 Cite