Keep Current with the Latest in Cell Biology Research

Human Anogenital Monocyte-Derived Dendritic Cells and langerin+cDC2 Are Major HIV Target Cells

Scientsts showed that HIV could penetrate the epithelial surface to interact with sub-epithelial resident mononuclear phagocytes in anogenital explants and defined the full array of subsets that were present in the human anogenital and colorectal tissues that HIV may encounter during sexual transmission.
[Nature Communications]
Rhodes, J. W., Botting, R. A., Bertram, K. M., Vine, E. E., Rana, H., Baharlou, H., Vegh, P., O’Neil, T. R., Ashhurst, A. S., Fletcher, J., Parnell, G. P., Graham, J. D., Nasr, N., Lim, J. J. K., Barnouti, L., Haertsch, P., Gosselink, M. P., Di Re, A., Reza, F., … Harman, A. N. (2021). Human anogenital monocyte-derived dendritic cells and langerin+cDC2 are major HIV target cells. Nature Communications, 12(1), 2147. https://doi.org/10.1038/s41467-021-22375-x Cite
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Atlas of Breast Cancer Infiltrated B-Lymphocytes Revealed by Paired Single-Cell RNA-Sequencing and Antigen Receptor Profiling

Researchers combined single-cell RNA-sequencing and antigen receptor lineage analysis to characterize a large number of triple-negative breast cancer infiltrated immune cells and report a comprehensive atlas of tumor-infiltrated B-lymphocytes.
[Nature Communications]
Hu, Q., Hong, Y., Qi, P., Lu, G., Mai, X., Xu, S., He, X., Guo, Y., Gao, L., Jing, Z., Wang, J., Cai, T., & Zhang, Y. (2021). Atlas of breast cancer infiltrated B-lymphocytes revealed by paired single-cell RNA-sequencing and antigen receptor profiling. Nature Communications, 12(1), 2186. https://doi.org/10.1038/s41467-021-22300-2 Cite
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Eotaxin-1/CCL11 Is Involved in Cell Migration in Rheumatoid Arthritis

The authors investigated the expression and function of Eotaxin-1 (CCL11) in rheumatoid arthritis fibroblast-like synoviocytes.
[Scientific Reports]
Wakabayashi, K., Isozaki, T., Tsubokura, Y., Fukuse, S., & Kasama, T. (2021). Eotaxin-1/CCL11 is involved in cell migration in rheumatoid arthritis. Scientific Reports, 11(1), 7937. https://doi.org/10.1038/s41598-021-87199-7 Cite
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Lipid Nanoparticle Encapsulated Nucleoside-Modified mRNA Vaccines Elicit Polyfunctional HIV-1 Antibodies Comparable to Proteins in Nonhuman Primates

Researchers showed that mRNA-LNP immunization compared to protein immunization elicits either the same or superior magnitude and breadth of HIV-1 Env-specific polyfunctional antibodies.
[npj Vaccines]
Saunders, K. O., Pardi, N., Parks, R., Santra, S., Mu, Z., Sutherland, L., Scearce, R., Barr, M., Eaton, A., Hernandez, G., Goodman, D., Hogan, M. J., Tombacz, I., Gordon, D. N., Rountree, R. W., Wang, Y., Lewis, M. G., Pierson, T. C., Barbosa, C., … Haynes, B. F. (2021). Lipid nanoparticle encapsulated nucleoside-modified mRNA vaccines elicit polyfunctional HIV-1 antibodies comparable to proteins in nonhuman primates. Npj Vaccines, 6(1), 1–14. https://doi.org/10.1038/s41541-021-00307-6 Cite
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A Highly Multiplexed Droplet Digital PCR Assay to Measure the Intact HIV-1 Proviral Reservoir

Scientists designed two triplex droplet digital PCR assays, each with two unique targets and one in common, and normalize the results to PCR-based T cell counts.
[Cell Reports Medicine]
Levy, C. N., Hughes, S. M., Roychoudhury, P., Reeves, D. B., Amstuz, C., Zhu, H., Huang, M.-L., Wei, Y., Bull, M. E., Cassidy, N. A. J., McClure, J., Frenkel, L. M., Stone, M., Bakkour, S., Wonderlich, E. R., Busch, M. P., Deeks, S. G., Schiffer, J. T., Coombs, R. W., … Hladik, F. (2021). A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir. Cell Reports Medicine, 0(0). https://doi.org/10.1016/j.xcrm.2021.100243 Cite
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Neutrophil Subtypes Shape HIV-Specific CD8 T-cell Responses after Vaccinia Virus Infection

Scientists found that these neutrophil subtypes showed distinct migratory and motility patterns and different ability to interact with CD8 T cells in the spleen following vaccinia virus infection.
[npj Vaccines]
Di Pilato, M., Palomino-Segura, M., Mejías-Pérez, E., Gómez, C. E., Rubio-Ponce, A., D’Antuono, R., Pizzagalli, D. U., Pérez, P., Kfuri-Rubens, R., Benguría, A., Dopazo, A., Ballesteros, I., Sorzano, C. O. S., Hidalgo, A., Esteban, M., & Gonzalez, S. F. (2021). Neutrophil subtypes shape HIV-specific CD8 T-cell responses after vaccinia virus infection. Npj Vaccines, 6(1), 1–11. https://doi.org/10.1038/s41541-021-00314-7 Cite
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Control of IFN-I Responses by the Aminopeptidase IRAP in Neonatal C57BL/6 Alveolar Macrophages during RSV Infection

Neonatal C57BL/6 alveolar macrophages mobilized very weakly the IFN-I pathway upon Respiratory Syncytial Virus (RSV) infection in vitro and failed to restrain virus replication.
[Mucosal Immunology]
Drajac, C., Laubreton, D., Marquant, Q., Chottin, C., Ferret, C., Bouguyon, E., Schwartz-Cornil, I., Saveanu, L., Riffault, S., & Descamps, D. (2021). Control of IFN-I responses by the aminopeptidase IRAP in neonatal C57BL/6 alveolar macrophages during RSV infection. Mucosal Immunology, 1–14. https://doi.org/10.1038/s41385-021-00402-w Cite
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Human Airway Cells Prevent SARS-CoV-2 Multibasic Cleavage Site Cell Culture Adaptation

Researchers report that propagating SARS-CoV-2 on the human airway cell line Calu-3 prevented cell culture adaptations in the multibasic cleavage sites. Similar results were obtained using a human airway organoid-based culture system for SARS-CoV-2 propagation.
[eLife]
Lamers, M. M., Mykytyn, A. Z., Breugem, T. I., Wang, Y., Wu, D. C., Riesebosch, S., van den Doel, P. B., Schipper, D., Bestebroer, T., Wu, N. C., & Haagmans, B. L. (2021). Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation. ELife, 10, e66815. https://doi.org/10.7554/eLife.66815 Cite
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Adhesion Receptor Adgrg2/Gpr64 Is In The Gi-Tract Selectively Expressed In Mature Intestinal Tuft Cells

Time-lapse studies of organoids from Trpm5GFP:Gpr64mCherry mice revealed sequential expression of initially Trpm5GFP and subsequently also Gpr64mCherry in maturing intestinal tuft cells.
[Molecular Metabolism]
Grunddal, K. V., Tonack, S., Egerod, K. L., Thompson, J. J., Petersen, N., Engelstoft, M. S., Vagne, C., Keime, C., Gradwohl, G., Offermanns, S., & Schwartz, T. W. (2021). Adhesion Receptor Adgrg2/Gpr64 Is In The Gi-Tract Selectively Expressed In Mature Intestinal Tuft Cells. Molecular Metabolism, 101231. https://doi.org/10.1016/j.molmet.2021.101231 Cite
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Improved Radium-223 Therapy with Combination Epithelial Sodium Channel Blockade

Utilizing primary human duodenal organoids as in vitro models of the functional GI epithelium, researchers found that Amiloride and NS-1619 presented significant effects in 223Ra membranal transport.
[Journal of Nuclear Medicine]
Abou, D. S., Fears, A., Summer, L., Longtine, M., Benabdallah, N., Riddle, R. C., Ulmert, D., Michalski, J., Wahl, R. L., Chesner, D., Doucet, M., Zachos, N., Simons, B., & Thorek, D. L. (2021). Improved Radium-223 Therapy with Combination Epithelial Sodium Channel Blockade. Journal of Nuclear Medicine. https://doi.org/10.2967/jnumed.121.261977 Cite
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Patient-Derived Organoids as a Predictive Biomarker for Treatment Response in Cancer Patients

The authors review and provide a pooled analysis of the results regarding the use of patient-derived organoids in individualized tumour response testing, focusing on evidence for analytical validity, clinical validity and clinical utility.
[npj Precision Oncology]
Wensink, G. E., Elias, S. G., Mullenders, J., Koopman, M., Boj, S. F., Kranenburg, O. W., & Roodhart, J. M. L. (2021). Patient-derived organoids as a predictive biomarker for treatment response in cancer patients. Npj Precision Oncology, 5(1), 1–13. https://doi.org/10.1038/s41698-021-00168-1 Cite
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