The authors report an electroporation-mediated plasmid CRISPR/Cas9 delivery approach for genome editing in iPSCs. With this approach, an edited iPSC cell line could be obtained within two weeks.
[Acta Pharmacologica Sinica]
Geng, B., Choi, K.-H., Wang, S., Chen, P., Pan, X., Dong, N., Ko, J.-K., & Zhu, H. (2020). A simple, quick, and efficient CRISPR/Cas9 genome editing method for human induced pluripotent stem cells. Acta Pharmacologica Sinica, 1–6. https://doi.org/10.1038/s41401-020-0452-0 Cite
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