Keep Current with the Latest in Cell Biology Research

A Simple, Quick, and Efficient CRISPR/Cas9 Genome Editing Method for Human Induced Pluripotent Stem Cells

The authors report an electroporation-mediated plasmid CRISPR/Cas9 delivery approach for genome editing in iPSCs. With this approach, an edited iPSC cell line could be obtained within two weeks.
[Acta Pharmacologica Sinica]
Geng, B., Choi, K.-H., Wang, S., Chen, P., Pan, X., Dong, N., Ko, J.-K., & Zhu, H. (2020). A simple, quick, and efficient CRISPR/Cas9 genome editing method for human induced pluripotent stem cells. Acta Pharmacologica Sinica, 1–6. https://doi.org/10.1038/s41401-020-0452-0 Cite
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BRPF3-HUWE1-Mediated Regulation of MYST2 Is Required for Differentiation and Cell-Cycle Progression in Embryonic Stem Cells

Researchers provided novel insights into understanding the functions of Brpf3 in proper differentiation as well as cell-cycle progression of ESCs via regulation of Myst2 stability by obstructing Huwe1-mediated ubiquitination.
[Cell Death & Differentiation]
Cho, H. I., Kim, M. S., Lee, J., Yoo, B. C., Kim, K. H., Choe, K.-M., & Jang, Y. K. (2020). BRPF3-HUWE1-mediated regulation of MYST2 is required for differentiation and cell-cycle progression in embryonic stem cells. Cell Death & Differentiation, 1–16. https://doi.org/10.1038/s41418-020-0577-1 Cite
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Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis

The authors suggested that urea cycle hyperreactivity and excessive polyamine generation in psoriatic keratinocytes promoted self-RNA sensation and highlighted protein phosphatase 6 deregulation in keratinocytes as a pivotal event that amplified the inflammatory circuits in psoriasis.
[Immunity]
Lou, F., Sun, Y., Xu, Z., Niu, L., Wang, Z., Deng, S., Liu, Z., Zhou, H., Bai, J., Yin, Q., Cai, X., Sun, L., Wang, H., Li, Q., Wu, Z., Chen, X., Gu, J., Shi, Y.-L., Tao, W., … Wang, H. (2020). Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis. Immunity, 0(0). https://doi.org/10.1016/j.immuni.2020.06.004 Cite
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Disseminated Melanoma Cells Transdifferentiate into Endothelial Cells in Intravascular Niches at Metastatic Sites

Researchers combined lineage tracing in a spontaneous metastatic mouse model of melanoma, advanced imaging, and single-cell RNA sequencing approaches to search for pathophysiologically relevant melanoma cellular states.
[Cell Reports]
Li, X., Karras, P., Torres, R., Rambow, F., Oord, J. van den, Marine, J.-C., & Kos, L. (2020). Disseminated Melanoma Cells Transdifferentiate into Endothelial Cells in Intravascular Niches at Metastatic Sites. Cell Reports, 31(11). https://doi.org/10.1016/j.celrep.2020.107765 Cite
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US Science Groups Wary of New Senate Bills to Curb Foreign Influences

A bipartisan group of US senators proposed sweeping—and controversial—changes in how the federal government manages academic research in the face of threats from other countries.
[ScienceInsider]
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ADP Receptor P2y12 Prevents Excessive Primitive Hematopoiesis in Zebrafish by Inhibiting Gata1

By using mass spectrometry combined with RNA sequencing, scientists showed that absence of P2y12 induced excessive erythropoiesis, evidenced by significantly increased expression of mature erythrocytes marker α-globin, β-globin.
[Acta Pharmacologica Sinica]
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Targeting Lung Cancer Stem Cells Using Combination of Tel and Docetaxel Liposomes in 3D Cultures and Tumor Xenografts

Scientists demonstrated for the first time the efficacy of Docetaxel liposomes in combination with telmisartan in 3D cultures of H460 cells by using polysaccharide-based hydrogels and also in xenograft model of Docetaxel resistant H460 derived CD133+ lung tumors.
[Toxicology and Applied Pharmacology]
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Emmes Announces Its Work on a Phase III Clinical Trial for Breakthrough Therapy Designation Product

Emmes announced that it provided the Phase III clinical trial support for a new investigational cell therapy that offers a promising treatment opportunity for patients who need an allogeneic bone marrow transplant.
[Emmes (PR Newswire, Inc.)]
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Allogeneic Cord Blood Transfusions Prevent Fetal Haemoglobin Depletion in Preterm Neonates. Results of the CB-TrIP Study

Investigators monitored fetal haemoglobin levels in 25 preterm neonates until 36 weeks of post‐menstrual age; patients received repeated red blood cell (RBC) units from allogeneic cord blood (cord‐RBCs) or from adult donors, depending on whether cord‐RBCs were available.
[British Journal of Haematology]
Teofili, L., Papacci, P., Orlando, N., Bianchi, M., Molisso, A., Purcaro, V., … Stefano, V. D. (n.d.). Allogeneic cord blood transfusions prevent fetal haemoglobin depletion in preterm neonates. Results of the CB-TrIP study. British Journal of Haematology, n/a(n/a). https://doi.org/10.1111/bjh.16851 Cite
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Hypoxia-Preconditioned Placenta-Derived Mesenchymal Stem Cells Rescue Optic Nerve Axons via Differential Roles of Vascular Endothelial Growth Factor in an Optic Nerve Compression Animal Model

Scientists established a hypoxia-preconditioned strain using a hypoxic chamber. The human placenta–derived stem cells were exposed to short-term hypoxic conditions of 2.2% O2 and 5.5% CO2.
[Molecular Neurobiology]
Kwon, H., Park, M., Nepali, S., & Lew, H. (2020). Hypoxia-Preconditioned Placenta-Derived Mesenchymal Stem Cells Rescue Optic Nerve Axons Via Differential Roles of Vascular Endothelial Growth Factor in an Optic Nerve Compression Animal Model. Molecular Neurobiology. https://doi.org/10.1007/s12035-020-01965-8 Cite
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Plasma Membrane Vesicles of Human Umbilical Cord Mesenchymal Stem Cells Ameliorate Acetaminophen-Induced Damage in HepG2 Cells: A Novel Stem Cell Therapy

PMVs from human umbilical cord (hUC) MSCs were prepared with an improved procedure including a chemical enucleation step followed by a mechanical extrusion. Plasma membrane vesicles of hUCMSCs were characterized and supplemented to hepatocyte cultures.
[Stem Cell Research & Therapy]
Lin, M., Li, S., Yang, L., Ye, D., Xu, L., Zhang, X., … Wei, C. (2020). Plasma membrane vesicles of human umbilical cord mesenchymal stem cells ameliorate acetaminophen-induced damage in HepG2 cells: a novel stem cell therapy. Stem Cell Research & Therapy, 11(1), 225. https://doi.org/10.1186/s13287-020-01738-z Cite
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