Normal human kidneys were obtained from radically nephrectomised patients with urogenital malignancy. Subsequently, human kidney immune cell subsets were analysed using multicolor flow cytometry and compared with subsets from C57BL/6 or BALB/c mice under specific pathogen-free conditions.
Park, J.-G., Na, M., Kim, M.-G., Park, S. H., Lee, H. J., Kim, D. K., Kwak, C., Kim, Y. S., Chang, S., Moon, K. C., Lee, D.-S., & Han, S. S. (2020). Immune cell composition in normal human kidneys. Scientific Reports, 10(1), 15678. https://doi.org/10.1038/s41598-020-72821-xCite
With murine and humanized MHC–/–HLA-A2+DR4+ murine models of chronic graft-versus-host disease (cGVHD), researchers showed that both murine and human PSGL1loCD4+ T cells from GVHD target tissues have features of B cell helpers with upregulated-expression of PD1 and ICOS and production of IL-21.
Data sets revealed that B cells in the blood were not in homeostasis with compartments in other tissues. Investigators found striking donor-to-donor variability in the frequencies and isotype of CD27+ memory B cells
Weisel, N. M., Weisel, F. J., Farber, D. L., Borghesi, L., Shen, Y., Ma, W., Luning Prak, E. T., & Shlomchik, M. (n.d.). Comprehensive analyses of B cell compartments across the human body reveal novel subsets and a gut resident memory phenotype. Blood. https://doi.org/10.1182/blood.2019002782Cite
Allografts were rapidly infiltrated by recipient leukocytes, which recapitulated the liver myeloid and lymphoid composition, and underwent partial reprogramming with acquisition of CD68/CD206 on MNPs and CD69/CD103 on T cells.