BMP2-Dependent Gene Regulatory Network Analysis Reveals Klf4 as a Novel Transcription Factor of Osteoblast Differentiation

The BMP signaling pathway is widely regarded as one of the most important pathways in vertebrate skeletal biology, of which BMP2 is a potent inducer, governing the osteoblast differentiation of bone marrow stromal cells. Scientists used RNA-seq, ATAC-seq, and animal models to characterize the BMP2-dependent gene regulatory network governing osteoblast lineage commitment.
[Cell Death & Disease]
Yu, S., Guo, J., Sun, Z., Lin, C., Tao, H., Zhang, Q., Cui, Y., Zuo, H., Lin, Y., Chen, S., Liu, H., & Chen, Z. (2021). BMP2-dependent gene regulatory network analysis reveals Klf4 as a novel transcription factor of osteoblast differentiation. Cell Death & Disease, 12(2), 1–17. https://doi.org/10.1038/s41419-021-03480-7 Cite
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MEF2C Shapes the Microtranscriptome during Differentiation of Skeletal Muscles

Deep sequencing of small RNAs of human muscle cells revealed a set of miRNAs, including several muscle-specific miRNAs, that were sensitive to myocyte enhancer factor 2C (MEF2C) depletion.
[Scientific Reports]
Piasecka, A., Sekrecki, M., Szcześniak, M. W., & Sobczak, K. (2021). MEF2C shapes the microtranscriptome during differentiation of skeletal muscles. Scientific Reports, 11(1), 3476. https://doi.org/10.1038/s41598-021-82706-2 Cite
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Myocyte-Specific Enhancer Factor 2c Triggers Transdifferentiation of Adipose Tissue-Derived Stromal Cells into Spontaneously Beating Cardiomyocyte-Like Cells

Investigators developed a simple culture protocol under which the adult murine inguinal adipose tissue-derived stromal vascular fraction (Ad-SVF) reproducibly transdifferentiates into beating cardiomyocyte (CMs) without induction. The beating CMs showed the striated ventricular phenotype of cardiomyocytes and synchronised oscillation of the intracellular calcium concentration among cells on day 28 of Ad-SVF primary culture.
[Scientific Reports]
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Modeling Human TBX5 Haploinsufficiency Predicts Regulatory Networks for Congenital Heart Disease

The authors define transcriptional consequences of reduced dosage of the CHD transcription factor, TBX5, in individual cells during cardiomyocyte differentiation from human iPSCs.
[Developmental Cell]
Kathiriya, I. S., Rao, K. S., Iacono, G., Devine, W. P., Blair, A. P., Hota, S. K., Lai, M. H., Garay, B. I., Thomas, R., Gong, H. Z., Wasson, L. K., Goyal, P., Sukonnik, T., Hu, K. M., Akgun, G. A., Bernard, L. D., Akerberg, B. N., Gu, F., Li, K., … Bruneau, B. G. (2020). Modeling Human TBX5 Haploinsufficiency Predicts Regulatory Networks for Congenital Heart Disease. Developmental Cell, 0(0). https://doi.org/10.1016/j.devcel.2020.11.020 Cite
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Modeling Human TBX5 Haploinsufficiency Predicts Regulatory Networks for Congenital Heart Disease

Researchers define transcriptional consequences of reduced dosage of the congenital heart disease transcription factor, TBX5, in individual cells during cardiomyocyte differentiation from human induced pluripotent stem cells.
[Developmental Cell]
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Critical Regulation of a NDIME/MEF2C Axis in Embryonic Stem Cell Neural Differentiation and Autism

The authors report that a brain‐enriched long non‐coding RNA, NDIME, is located near the MEF2C locus and is required for normal neural differentiation of mouse ESCs.
[EMBO Reports]
Bai, M., Ye, D., Guo, X., Xi, J., Liu, N., Wu, Y., Jia, W., Wang, G., Chen, W., Li, G., Jiapaer, Z., & Kang, J. (2020). Critical regulation of a NDIME/MEF2C axis in embryonic stem cell neural differentiation and autism. EMBO Reports, n/a(n/a), e50283. https://doi.org/10.15252/embr.202050283 Cite
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Poly(ADP-Ribose) Polymerase 1 (PARP1) Restrains MyoD-Dependent Gene Expression during Muscle Differentiation

The authors observed that Poly(ADP-ribose) Polymerase 1 (PARP1) depletion boosted the up-regulation of MyoD targets, such as p57, myogenin, Mef2C and p21, while its re-expression reverted this effect. They also found that PARP1 interacted with some MyoD-binding regions and that its presence, independently of the enzymatic activity, interfered with MyoD recruitment and gene induction.
[Scientific Reports]
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A Small Molecular Compound CC1007 Induces Cross-Lineage Differentiation by Inhibiting HDAC7 Expression and HDAC7/MEF2C Interaction in BCR-ABL Pre-B-ALL

The authors investigated the anticancer effects of CC1007 in breakpoint cluster region-Abelson 1 fusion gene-negative (BCR-ABL1) pre-B-ALL cell lines and primary patient-derived BCR-ABL1 pre-B-ALL cells.
[Cell Death & Disease]
Wang, Z., Zhang, Y., Zhu, S., Peng, H., Chen, Y., Cheng, Z., Liu, S., Luo, Y., Li, R., Deng, M., Xu, Y., Hu, G., Chen, L., & Zhang, G. (2020). A small molecular compound CC1007 induces cross-lineage differentiation by inhibiting HDAC7 expression and HDAC7/MEF2C interaction in BCR-ABL1 − pre-B-ALL. Cell Death & Disease, 11(9), 1–13. https://doi.org/10.1038/s41419-020-02949-1 Cite
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Genome-Wide Studies Reveal the Essential and Opposite Roles of ARID1A in Controlling Human Cardiogenesis and Neurogenesis from Pluripotent Stem Cells

The authors report ARID1A, a DNA-binding subunit of the SWI/SNF epigenetic complex, controls both neurogenesis and cardiogenesis from human embryonic stem cells through distinct mechanisms.
[Genome Biology]
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