In vitro infection of the T24 cell line with E. coli was performed to study the bacterial impact on bladder cancer cells. EMT markers were assessed using immunohistochemistry, western blot and RT-PCR. Stemness characteristics were monitored using RT-PCR.
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Abd-El-Raouf, R., Ouf, S. A., Gabr, M. M., Zakaria, M. M., El-Yasergy, K. F., & Ali-El-Dein, B. (2020). Escherichia coli foster bladder cancer cell line progression via epithelial mesenchymal transition, stemness and metabolic reprogramming. Scientific Reports, 10(1), 18024. https://doi.org/10.1038/s41598-020-74390-5 Cite
Investigators found in breast tumors that the expression of SMAR1 was decreased in cancer stem cells through the cooperative interaction of the pluripotency factors Oct4 and Sox2 with the histone deacetylase HDAC1.
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SMAR1 repression by pluripotency factors and consequent chemoresistance in breast cancer stem-like cells is reversed by aspirin | Science Signaling. (n.d.). Retrieved October 20, 2020, from https://stke.sciencemag.org/content/13/654/eaay6077 Cite
The neural crest (NC) is an ESC population that contributes to a greatly expanding list of derivatives ranging from neurons and glia of the peripheral nervous system, facial cartilage and bone, pigment cells of the skin to secretory cells of the endocrine system. Scientists focus on what is specifically known about establishment and maintenance of NC stemness and ultimate fate commitment mechanisms.
On one side, JMJD3 induced the pro-senescence factor Ink4a and degraded the pluripotency regulator PHF20 in a reprogramming factor-independent manner. On the other side, JMJD3 was specifically recruited by KLF4 to reduce H3K27me3 at both enhancers and promoters of epithelial and pluripotency genes.
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Huang, Y., Zhang, H., Wang, L., Tang, C., Qin, X., Wu, X., Pan, M., Tang, Y., Yang, Z., Babarinde, I. A., Lin, R., Ji, G., Lai, Y., Xu, X., Su, J., Wen, X., Satoh, T., Ahmed, T., Malik, V., … Qin, B. (2020). JMJD3 acts in tandem with KLF4 to facilitate reprogramming to pluripotency. Nature Communications, 11(1), 5061. https://doi.org/10.1038/s41467-020-18900-z Cite
The authors investigated the early transcriptional events of cellular reprogramming triggered by the co‐expression of Oct4, Sox2, Klf4, and c‐Myc in mouse embryonic fibroblasts and mouse hepatocytes.
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Papathanasiou, M., Tsiftsoglou, S. A., Polyzos, A. P., Papadopoulou, D., Valakos, D., Klagkou, E., Karagianni, P., Pliatska, M., Talianidis, I., Agelopoulos, M., & Thanos, D. (2020). Identification of a dynamic gene regulatory network required for pluripotency factor-induced reprogramming of mouse fibroblasts and hepatocytes. The EMBO Journal, n/a(n/a), e102236. https://doi.org/10.15252/embj.2019102236 Cite
Methanol extracts of carrot root were purified by means of silica gel, Sephadex LH-20, and preparative high-performance liquid chromatography to isolate a compound targeting mammosphere formation.
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Researchers investigated changes in expression levels of the cancer stem cell (CSC) biomarker, cluster of differentiation 133 (CD133), from primary ovarian cancer (OC) cell lines to induction of CSC-spheres in an attempt to explore the mechanisms related to modulation of stemness, drug resistance, and tumorigenesis in CSCs, thus facilitating the search for new therapeutics for OC.
[International Journal of Molecular Sciences]
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Using affinity purification and mass spectrometry analysis, investigators identified Kap1 as an Oct4-binding protein. Silencing of Kap1 reduced the protein levels of Oct4 in ESCs, whereas the overexpression of Kap1 stimulated the levels of Oct4.
[Cell Death & Differentiation]
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Cellular uptake and localization assays demonstrated that decoy Oct4–Sox2 complex decoy oligodeoxynucleotides could efficiently be transfected to the cells and resided in subcellular compartment, where they posed their action on gene regulation.
[Molecular Biology Reports]
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Johari, B., Rezaeejam, H., Moradi, M., Taghipour, Z., Saltanatpour, Z., Mortazavi, Y., & Nasehi, L. (2020). Increasing the colon cancer cells sensitivity toward radiation therapy via application of Oct4–Sox2 complex decoy oligodeoxynucleotides. Molecular Biology Reports. https://doi.org/10.1007/s11033-020-05737-4 Cite
Authors illustrate the mechanism by which OCT4 plays a role in cancer stem cells from the perspective of genetic modification of OCT4, non-coding RNA, complexes and signaling pathways associated with OCT4.
[International Journal of Stem Cells]
Researchers investigated whether sulforaphane could enhance the chemotherapeutic effects of cisplatin and 5-fluorouracil against head and neck squamous cell carcinomas–cancer stem cells, and its mechanisms of action.
[British Journal of Cancer]
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