Scientists proposed a novel mechanism for transcription factor EB (TFEB) governing pluripotency of mESCs by regulating the pluripotency transcriptional network.
[Cell Death & Disease]
The authors reviewed the molecular basis of reprogramming, including the reprogramming factors, applied vectors and epigenetic modifications to provide a comprehensive guide for reprogramming studies.
Researchers identified a clinically relevant signaling nexus mediated by AXL receptor, PYK2 and PKCα and show its impact on stemness in triple-negative breast cancer.
[Life Science Alliance]
The authors identified E26 transformation-specific homologous factor as a key molecule in decreasing the sensitivity of pancreatic cancer cells to cancer stem cells’ niche stimulus.
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Zhou, T., Liu, J., Xie, Y., Yuan, S., Guo, Y., Bai, W., Zhao, K., Jiang, W., Wang, H., Wang, H., Zhao, T., Huang, C., Gao, S., Wang, X., Yang, S., & Hao, J. (2021). ESE3/EHF, a promising target of rosiglitazone, suppresses pancreatic cancer stemness by downregulating CXCR4. Gut. https://doi.org/10.1136/gutjnl-2020-321952 Cite
Researchers provided a mass spectrometry–based approach for simultaneous and quantitative monitoring of transcription factors, in an attempt to provide insight into their contributions in hESC differentiation.
[Analytical and Bioanalytical Chemistry]
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Xu, M., Xu, L., Cao, J., Hu, Y., Xu, F., Liu, Y., & Chen, Y. (2021). Simultaneous and quantitative monitoring transcription factors in human embryonic stem cell differentiation using mass spectrometry–based targeted proteomics. Analytical and Bioanalytical Chemistry. https://doi.org/10.1007/s00216-021-03160-7 Cite
The authors showed that deficiency of retinoblastoma binding protein 4 (RBBP4), a component of the Polycomb repressive complex 2 in ESCs led to spontaneous differentiation into mesendodermal lineages.
[Stem Cell Reports]
Matrin3 is involved in the differentiation of neural cells and, here, the authors elucidate its critical functions in regulating pluripotent circuits in human iPSCs.
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Pollini, D., Loffredo, R., Maniscalco, F., Cardano, M., Micaelli, M., Bonomo, I., Licata, N. V., Peroni, D., Tomaszewska, W., Rossi, A., Crippa, V., Dassi, E., Viero, G., Quattrone, A., Poletti, A., Conti, L., & Provenzani, A. (2021). Multilayer and MATR3-dependent regulation of OCT4 NANOG and LIN28A maintains human pluripotency. IScience, 0(0). https://doi.org/10.1016/j.isci.2021.102197 Cite
Scientists used multiple colorectal cancer (CRC) cell lines to investigate the growth-inhibitory effect of crenolanib and its effect in combination with other cytotoxic agents. Primary cultures of patient-derived organoids, a model that reflects the heterogeneity of clinical CRC, were used to further validate the effects of crenolanib.
[Molecular Cancer Research]
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Fujino, S., Miyoshi, N., Ito, A., Yasui, M., Ohue, M., Ogino, T., Takahashi, H., Uemura, M., Matsuda, C., Mizushima, T., Doki, Y., & Eguchi, H. (2021). Crenolanib regulates ERK and AKT/mTOR signaling pathways in RAS/BRAF-mutated colorectal cancer cells and organoids. Molecular Cancer Research. https://doi.org/10.1158/1541-7786.MCR-20-0600 Cite
Induced pluripotency provides a tool to explore mechanisms underlying establishment, maintenance and differentiation of naïve pluripotent stem cells (nPSCs). The authors report that self-renewal of nPSCs requires minimal Sox2 expression.
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Open chromatin landscapes of Oct4+ cranial neural crest cells precursors resemble those of epiblast stem cells, with additional features suggestive of priming for mesenchymal programs.
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Investigators provided the first demonstration that farnesyl dimethyl chromanol inhibited colon cancer stem cell viability, survival, self-renewal, pluripotent transcription factors expression, organoids formation, and Wnt/β-catenin signalling, as evidenced by comparisons with vehicle-treated controls.
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