TFEB Regulates Pluripotency Transcriptional Network in Mouse Embryonic Stem Cells Independent of Autophagy–Lysosomal Biogenesis

Scientists proposed a novel mechanism for transcription factor EB (TFEB) governing pluripotency of mESCs by regulating the pluripotency transcriptional network.
[Cell Death & Disease]
Tan, A., Prasad, R., & Jho, E. (2021). TFEB regulates pluripotency transcriptional network in mouse embryonic stem cells independent of autophagy–lysosomal biogenesis. Cell Death & Disease, 12(4), 1–14. https://doi.org/10.1038/s41419-021-03632-9 Cite
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Induced Pluripotent Stem Cell Technology: Trends in Molecular Biology, from Genetics to Epigenetics

The authors reviewed the molecular basis of reprogramming, including the reprogramming factors, applied vectors and epigenetic modifications to provide a comprehensive guide for reprogramming studies.
[Epigenomics]
Maali, A., Maroufi, F., Sadeghi, F., Atashi, A., Kouchaki, R., Moghadami, M., & Azad, M. (2021). Induced pluripotent stem cell technology: trends in molecular biology, from genetics to epigenetics. Epigenomics. https://doi.org/10.2217/epi-2020-0409 Cite
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The AXL-PYK2-PKCα Axis as a Nexus of Stemness Circuits in TNBC

Researchers identified a clinically relevant signaling nexus mediated by AXL receptor, PYK2 and PKCα and show its impact on stemness in triple-negative breast cancer.
[Life Science Alliance]
Khera, L., Vinik, Y., Maina, F., & Lev, S. (2021). The AXL-PYK2-PKCα axis as a nexus of stemness circuits in TNBC. Life Science Alliance, 4(6). https://doi.org/10.26508/lsa.202000985 Cite
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ESE3/EHF, a Promising Target of Rosiglitazone, Suppresses Pancreatic Cancer Stemness by Downregulating CXCR4

The authors identified E26 transformation-specific homologous factor as a key molecule in decreasing the sensitivity of pancreatic cancer cells to cancer stem cells’ niche stimulus.
[Gut]
Zhou, T., Liu, J., Xie, Y., Yuan, S., Guo, Y., Bai, W., Zhao, K., Jiang, W., Wang, H., Wang, H., Zhao, T., Huang, C., Gao, S., Wang, X., Yang, S., & Hao, J. (2021). ESE3/EHF, a promising target of rosiglitazone, suppresses pancreatic cancer stemness by downregulating CXCR4. Gut. https://doi.org/10.1136/gutjnl-2020-321952 Cite
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Simultaneous and Quantitative Monitoring Transcription Factors in Human Embryonic Stem Cell Differentiation Using Mass Spectrometry–Based Targeted Proteomics

Researchers provided a mass spectrometry–based approach for simultaneous and quantitative monitoring of transcription factors, in an attempt to provide insight into their contributions in hESC differentiation.
[Analytical and Bioanalytical Chemistry]
Xu, M., Xu, L., Cao, J., Hu, Y., Xu, F., Liu, Y., & Chen, Y. (2021). Simultaneous and quantitative monitoring transcription factors in human embryonic stem cell differentiation using mass spectrometry–based targeted proteomics. Analytical and Bioanalytical Chemistry. https://doi.org/10.1007/s00216-021-03160-7 Cite
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Rbbp4 Suppresses Premature Differentiation of Embryonic Stem Cells

The authors showed that deficiency of retinoblastoma binding protein 4 (RBBP4), a component of the Polycomb repressive complex 2 in ESCs led to spontaneous differentiation into mesendodermal lineages.
[Stem Cell Reports]
Huang, Y., Su, T., Wang, C., Dong, L., Liu, S., Zhu, Y., Hao, K., Xia, Y., Jiang, Q., & Qin, J. (2021). Rbbp4 suppresses premature differentiation of embryonic stem cells. Stem Cell Reports, 0(0). https://doi.org/10.1016/j.stemcr.2021.01.009 Cite
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Multilayer and MATR3-Dependent Regulation of OCT4 NANOG and LIN28A Maintains Human Pluripotency

Matrin3 is involved in the differentiation of neural cells and, here, the authors elucidate its critical functions in regulating pluripotent circuits in human iPSCs.
[iScience]
Pollini, D., Loffredo, R., Maniscalco, F., Cardano, M., Micaelli, M., Bonomo, I., Licata, N. V., Peroni, D., Tomaszewska, W., Rossi, A., Crippa, V., Dassi, E., Viero, G., Quattrone, A., Poletti, A., Conti, L., & Provenzani, A. (2021). Multilayer and MATR3-dependent regulation of OCT4 NANOG and LIN28A maintains human pluripotency. IScience, 0(0). https://doi.org/10.1016/j.isci.2021.102197 Cite
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Crenolanib Regulates ERk and AKT/mTOR Signaling Pathways in RAS/BRAF-Mutated Colorectal Cancer Cells and Organoids

Scientists used multiple colorectal cancer (CRC) cell lines to investigate the growth-inhibitory effect of crenolanib and its effect in combination with other cytotoxic agents. Primary cultures of patient-derived organoids, a model that reflects the heterogeneity of clinical CRC, were used to further validate the effects of crenolanib.
[Molecular Cancer Research]
Fujino, S., Miyoshi, N., Ito, A., Yasui, M., Ohue, M., Ogino, T., Takahashi, H., Uemura, M., Matsuda, C., Mizushima, T., Doki, Y., & Eguchi, H. (2021). Crenolanib regulates ERK and AKT/mTOR signaling pathways in RAS/BRAF-mutated colorectal cancer cells and organoids. Molecular Cancer Research. https://doi.org/10.1158/1541-7786.MCR-20-0600 Cite
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Sox2 Modulation Increases Naïve Pluripotency Plasticity

Induced pluripotency provides a tool to explore mechanisms underlying establishment, maintenance and differentiation of naïve pluripotent stem cells (nPSCs). The authors report that self-renewal of nPSCs requires minimal Sox2 expression.
[iScience]
Tremble, K., Stirparo, G. G., Bates, L. E., Maskalenka, K., Stuart, H. T., Jones, K., Andersson-Rolf, A., Radzisheuskaya, A., Koo, B.-K., Bertone, P., & Silva, J. C. R. (2021). Sox2 modulation increases naïve pluripotency plasticity. IScience, 0(0). https://doi.org/10.1016/j.isci.2021.102153 Cite
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Reactivation of the Pluripotency Program Precedes Formation of the Cranial Neural Crest

Open chromatin landscapes of Oct4+ cranial neural crest cells precursors resemble those of epiblast stem cells, with additional features suggestive of priming for mesenchymal programs.
[Science]
Zalc, A., Sinha, R., Gulati, G. S., Wesche, D. J., Daszczuk, P., Swigut, T., Weissman, I. L., & Wysocka, J. (2021). Reactivation of the pluripotency program precedes formation of the cranial neural crest. Science, 371(6529). https://doi.org/10.1126/science.abb4776 Cite
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Farnesyl Dimethyl Chromanol Targets Colon Cancer Stem Cells and Prevents Colorectal Cancer Metastasis

Investigators provided the first demonstration that farnesyl dimethyl chromanol inhibited colon cancer stem cell viability, survival, self-renewal, pluripotent transcription factors expression, organoids formation, and Wnt/β-catenin signalling, as evidenced by comparisons with vehicle-treated controls.
[Scientific Reports]
Wijshake, T., Zou, Z., Chen, B., Zhong, L., Xiao, G., Xie, Y., Doench, J. G., Bennett, L., & Levine, B. (2021). Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin. Proceedings of the National Academy of Sciences, 118(5). https://doi.org/10.1073/pnas.2020478118 Cite
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