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PBMCs

Mitogen-Activated Protein Kinase Signaling in Childhood Asthma Development and Environment-Mediated Protection

[Pediatric Allergy and Immunology] Scientists aimed to investigate the role of MAPK signaling in childhood asthma and its environment-mediated protection, including a representative selection of 232 out of 1062 children from two cross-sectional cohorts and one birth cohort study.

Immune Normalization Strategy against Suboptimal Health Status: Safe and Efficacious Therapy Using Mixed-Natural Killer Cells

[Aging-Us] Researchers established in vitro cultured mixed-natural killer cells (NKM) to achieve immune normalization. The in vitro cytotoxicity of NKM cells was tenfold higher than that of peripheral blood mononuclear cells

Cas9-Derived Peptides Presented by MHC Class II That Elicit Proliferation of CD4+ T-cells

[Nature Communications] Investigators used an MHC Associated Peptide Proteomics assay to identify SaCas9 peptides presented by MHC Class II (MHC-II) proteins on dendritic cells.

Transcriptional Profiling of Circulating Mononuclear Cells from Patients with Chronic Obstructive Pulmonary Disease Receiving Mesenchymal Stromal Cell Infusions

[Stem Cells Translational Medicine] Allogeneic, bone marrow-derived MSCs were systemically administered into patients with stable chronic obstructive pulmonary disease. Gene expression profiles from peripheral blood mononuclear cells were analyzed across the first week after infusion.

Hantavirus Infection-Induced B Cell Activation Elevates Free Light Chains Levels in Circulation

[PLOS Pathogens] Researchers reported that acute orthohantavirus infection increases the level of Ig free light chains in serum of both hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome patients, and that the increase correlated with the severity of acute kidney injury in HFRS.

Dickkopf-Related Protein 3 Alters Aerobic Glycolysis in Pancreatic Cancer BxPC-3 Cells, Promoting CD4+ T-cell Activation and Function

[European Journal of Medical Research] The BxPC-3-DKK3 cell line was constructed, and peripheral blood mononuclear cell was prepared. After isolated the CD4+ T cells, the lactic acid, glucose uptake ability, cellular viability, proliferation, apoptosis, and markers were detected by PCR and western blot, and the concentrations of multiple cytokines were determined using the ELISA method.

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