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PBMCs

Single-Cell eQTL Mapping Identifies Cell Type-Specific Genetic Control of Autoimmune Disease

[Science] Researchers developed a framework for the classification of individual cells, and by combining single-cell RNA sequencing data with genotype data, they mapped the genetic effects on gene expression in each of 14 immune cell types and identified 26,597 independent cis–expression quantitative trait loci (eQTLs).

Overexpression of Multiple Epidermal Growth Factor Like Domains 11 Rescues Anoikis Survival through Tumor Cells-Platelet Interaction in Triple Negative Breast Cancer Cells

[Life Sciences] The role of multiple epidermal growth factor like domains 11 (MEGF11) was studied in human TNBC MDA-MB-231 and MDA-MB-468 cells by overexpressing MEGF11 using non-attached culture.

Rapid Cloning of Antigen-Specific TCell Receptors by Leveraging the Cis Activation of T Cells

[Nature Biomedical Engineering] By analyzing a large number of T cells at the single-cell level on a microwell array, scientists showed that T cell activation could occur via cis interactions, and that such cis activation could be used to detect antigen-specific T cells and clone their T cell receptor within four days.

Single-Cell Transcriptional Diversity of Neonatal Umbilical Cord Blood Immune Cells Reveals Neonatal Immune Tolerance

[Biochemical and Biophysical Research Communications] Scientists applied single-cell RNA sequencing and single-cell sequencing assay for transposase-accessible chromatin to systematically profile umbilical cord blood nucleated cells and peripheral blood mononuclear cells to identify their composition and differentially expressed genes.

Generation of Integration Free hiPSCs Clones, NSi001-A, NSi001-B, and NSi001-C from Peripheral Blood Mononuclear Cells of an Individual with Down Syndrome Having Robertsonian Translocation

[Stem Cell Research] Human hiPSC clones NSi001-A, NSi001-B, and NSi001-C were generated from a female individual of Indian origin having Robertsonian translocation down syndrome by reprogramming peripheral blood mononuclear cells using integration-free Sendai viral vectors.

The Impact of CCR8+ Regulatory T Cells on Cytotoxic T Cell Function in Human Lung Cancer

[Scientific Reports] The authors investigated the features of CCR8+ Tregs in lung cancer patients. CCR8+ Tregs were highly activated and infiltration of CCR8+ Tregs in tumors was associated with poor prognosis in lung cancer patients.

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