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RUNX1

Dynamic Runx1 Chromatin Boundaries Affect Gene Expression in Hematopoietic Development

[Nature Communications] To dissect the dynamic regulation of Runx1 in hematopoietic development, researchers analyzed its 3D chromatin conformation in mouse ESC differentiation cultures.

Runx1 Regulates Tff1 Expression to Expedite Viability of Retinal Microvascular Endothelial Cells in Mice with Diabetic Retinopathy

[Experimental Eye Research] Researchers generated a diabetic retinopathy (DR) animal model using mice and a cell model in mouse retinal microvascular endothelial cells to examine the role of Trefoil factor family 1 in DR.

Pre-Configuring Chromatin Architecture with Histone Modifications Guides Hematopoietic Stem Cell Formation in Mouse Embryos

[Nature Communications] The authors performed multi-omics dissection of the hematopoietic stem cell (HSC) ontogeny trajectory across early arterial endothelial cells (ECs), hemogenic ECs, pre-HSCs and long-term HSCs in mouse embryos.

Differentiation of Cancer Stem Cells into Erythroblasts in the Presence of CoCl2

[Scientific Reports] Researchers investigated the potential of CSC differentiation into blood cells under chemical hypoxic conditions using CoCl2. CSCs and miPS-LLCcm cells were cultured for one to seven days in the presence of CoCl2, and the expression of VEGFR1/2, Runx1, c-kit, CD31, CD34, and TER-119 was assessed by RT-qPCR, Western blotting and flow cytometry together with Wright-Giemsa staining and immunocytochemistry.

The Onset of Circulation Triggers a Metabolic Switch Required for Endothelial to Hematopoietic Transition

[Cell Reports] Investigators showed that in Ncx1−/− mouse embryos devoid of circulation, the HSC lineage developed until the phenotypic pro-HSC stage. Experimental activation of glycolysis resulted in decreased intraembryonic hematopoiesis, suggesting that the onset of circulation triggered metabolic changes that allowed HSC generation to proceed.

Regeneration of Immunocompetent B Lymphopoiesis from Pluripotent Stem Cells Guided by Transcription Factors

[Cellular & Molecular Immunology] Researchers unveiled the guiding role of three essential factors, Lhx2, Hoxa9, and Runx1, the simultaneous expression of which preferentially drove B lineage fate commitment and in vivo B lymphopoiesis using PSCs as a cell source.

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