cell culture

[Biofabrication] Scientists developed endothelial cell-laden pro- and anti-angiogenic bioinks, supplemented with bioactive matrix-derived microfibers that were created from type I collagen sponges and cartilage decellularized ECM.

[Oxidative Medicine and Cellular Longevity] Scientists explored the effect of the plasma-activated medium on lung cancer cells in vitro and in vivo by using a 3D cell culture model.

[Cell Death & Disease] Scientists detected the receptor tyrosine kinase activity in gefitinib-resistant colorectal cancer cells, pan-erb-b2 receptor tyrosine kinase 3 (ErbB3)/EGFR was significantly activated and provided a potential multi-ErbB treatment target.

[Slas Discovery] Investigators described the development and implementation of a regressive deep learning model trained on brightfield images of patient-derived organoids and use the terminal viability readout as training labels.

[Methods] Scientists designed a workflow to reproducibly extract proteins from brain organoids, measure global turnover using mass spectrometry, and statistically investigate turnover differences between genotypes.

[iScience] The authors applied high-dimensional design of experiments methodology to identify critical process parameters that govern neural territory patterning from pluripotency — the first stage toward specification of central nervous system cell fates.