cell culture

[Science] Scientists characterized leader cells emerging from epithelial monolayers in cell culture and found that they activated the stress sensor p53, which was sufficient to initiate leader cell behavior.

[American Journal of Physiology-Lung Cellular and Molecular Physiology] Investigators isolated primary alveolar type-2 cells (AEC2) from patient-derived lung tissues including those from patients with severe emphysema, using magnetic bead-based selection of cells expressing the AEC2 marker HTII-280.

[Nature Protocols] Researchers developed a protocol to visualize and quantify the spatiotemporal evolution of newly synthesized and deposited matrix by cells that are either cultured atop or embedded within biomaterial systems.

[Communications Biology] Scientists performed frequent in situ monitoring of dissolved O2 and CO2 with optical sensor spots and contemporaneous evaluation of cell proliferation and medium pH in standard batch cultures of three widely used human somatic and PSC lines.

[Acta Biomaterialia] Scientists examined the roles of surface topographies and cell-cell interactions in inducing cell alignment. They employed wavy surface topographies at the nanometer scale as a model extracellular environment for cell culture.

[Small] Scientists introduce the classification, design, and assembly of DNA hydrogels, and then summarize the state-of-the-art achievements in cell encapsulation, cell culture, and tissue engineering with DNA hydrogels.