Researchers used a cell-tagging dye, CFDA-SE, to permanently label pancreatic duct cells through an intraductal infusion technique.
[Experimental and Molecular Medicine]
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Investigators generated human serotonergic neurons in vitro and detected secreted serotonin using aptamer-coated nanopipettes in a low nanomolar range, with the possibility of detecting significantly lower concentrations.
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Exacis Biotherapeutics Inc., announced several important steps in the preclinical development of its ExaNK™ engineered NK cell-therapy candidates.
[Exacis Biotherapeutics Inc. (PR Newswire LLC.)]
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The authors highlight recent advances of neuronal organoid technologies, focusing on the region-specific brain organoids and complementation with endothelial cells and microglia
[Journal of Molecular Medicine]
Investigators demonstrated that in the adult mouse brain, small molecules could reprogram astrocytes into neurons.
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Ma, Y., Xie, H., Du, X., Wang, L., Jin, X., Zhang, Q., Han, Y., Sun, S., Wang, L., Li, X., Zhang, C., Wang, M., Li, C., Xu, J., Huang, Z., Wang, X., Chai, Z., & Deng, H. (2021). In vivo chemical reprogramming of astrocytes into neurons. Cell Discovery, 7(1), 1–13. https://doi.org/10.1038/s41421-021-00243-8 Cite
Investigators demonstrated that CYCLIN D1 made induced pluripotent stem cells have lower multitelomeric signal, reduced double‐strand DNA breaks, correct nuclear localization of RAD51 protein expression, and reduced SNP changes per chromosome, compared with the classical reprogramming method using C‐MYC.
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Scientists report that during the process of induced cell reprogramming, methylation content of the Ngn3 and Sox11 genes are diminished.
[npj Regenerative Medicine]
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One of the biggest challenges in drug development and personalized medicine is how to model the cellular microenvironment accurately. Cellaria and BioLamina are pursuing this challenge by collaborating to develop more defined reprogramming methods, large-scale iPSC expansions, and lineage differentiation for human cells and animal cells for vertebrate and non-vertebrate species.
[Cellaria Inc. and BioLamina (Businesswire, Inc.)]
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Researchers describe the first application of biochemically engineered ten-eleven translocation (TET) mutants that unlink 5-methylcytosine oxidation steps, examining their effects on somatic cell reprogramming.
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Caldwell, B. A., Liu, M. Y., Prasasya, R. D., Wang, T., DeNizio, J. E., Leu, N. A., Amoh, N. Y. A., Krapp, C., Lan, Y., Shields, E. J., Bonasio, R., Lengner, C. J., Kohli, R. M., & Bartolomei, M. S. (2020). Functionally distinct roles for TET-oxidized 5-methylcytosine bases in somatic reprogramming to pluripotency. Molecular Cell, 0(0). https://doi.org/10.1016/j.molcel.2020.11.045 Cite
Human mRNA transcript profiling at multiple time points permited the tracking of the reprogramming of B cell nuclei to a multipotent state. Interrogation of a human B cell regulatory network with gene expression signatures identified eight candidate master regulator proteins.
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Arumugam, K., Shin, W., Schiavone, V., Vlahos, L., Tu, X., Carnevali, D., Kesner, J., Paull, E. O., Romo, N., Subramaniam, P., Worley, J., Tan, X., Califano, A., & Cosma, M. P. (2020). The Master Regulator Protein BAZ2B Can Reprogram Human Hematopoietic Lineage-Committed Progenitors into a Multipotent State. Cell Reports, 33(10). https://doi.org/10.1016/j.celrep.2020.108474 Cite
Using multiplexed gene regulation with orthogonal CRISPR systems, researchers demonstrated improved neuronal differentiation with concurrent activation and repression of target genes, underscoring the power of CRISPR-based gene regulation for programming complex cellular phenotypes.
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Black, J. B., McCutcheon, S. R., Dube, S., Barrera, A., Klann, T. S., Rice, G. A., Adkar, S. S., Soderling, S. H., Reddy, T. E., & Gersbach, C. A. (2020). Master Regulators and Cofactors of Human Neuronal Cell Fate Specification Identified by CRISPR Gene Activation Screens. Cell Reports, 33(9). https://doi.org/10.1016/j.celrep.2020.108460 Cite