Novel Chemotherapeutic Agent FX-9 Activates NF-κB Signaling and Induces G1 Phase Arrest by Activating CDKN1A in a Human Prostate Cancer Cell Line

Investigators conducted microarray gene expression analysis and compared FX-9 exposed and unexposed prostate cancer cells, followed by pathway analysis and gene annotation to functional processes.
[BMC Cancer]
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Probing Multicellular Tissue Fusion of Cocultured Spheroids—A 3D-Bioassembly Model

A facile 3D-bioassembly platform was introduced to primarily study fusion of cartilage–cartilage interfaces using spheroids formed from human MSCs and articular chondrocytes.
[Advanced Science]
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BMP7 Reduces the Fibrocartilage Chondrocyte Phenotype

Scientists demonstrated that short-term exposure to BMP7 decreased the fibrocartilage chondrocyte phenotype. The BMP7-dependent reduction of Collagen type I protein expression seemed MMP2-dependent and inhibition of Smad2/3-PAI1 activity was identified as a potential pathway via which BMP7 exerted its anti-fibrotic action.
[Scientific Reports]
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Human Pluripotent Stem Cell-Derived Cartilaginous Organoids Promote Scaffold-Free Healing of Critical Size Long Bone Defects

The in vitro and in vivo cartilage and bone tissue formation potencies of human PSCs were investigated. The iPSCs were specified towards the mesodermal lineage and differentiated towards chondrocytes, which subsequently self-assembled into cartilaginous organoids.
[Stem Cell Research & Therapy]
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A Scaffold-Free Approach to Cartilage Tissue Generation Using Human Embryonic Stem Cells

Researchers developed a robust method to generate 3D, scaffold-free, hyaline cartilage tissue constructs from human ESCs that were composed of numerous chondrocytes in lacunae, embedded in an extracellular matrix containing Type II collagen, sulphated glycosaminoglycans and Aggrecan.
[Scientific Reports]
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Fibroblast Growth Factor 21 (FGF21) Alleviates Senescence, Apoptosis, and Extracellular Matrix Degradation in Osteoarthritis via the SIRT1-mTOR Signaling Pathway

The role of FGF21 in the progression of osteoarthritis was investigated in both in vitro and in vivo experiments. In vitro, FGF21 administration alleviated apoptosis, senescence, and ECM catabolism of the chondrocytes induced by tert-butyl hydroperoxide by mediating autophagy flux.
[Cell Death & Disease]
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Rutin Inhibited the Advanced Glycation End Products-Stimulated Inflammatory Response and Extracellular Matrix Degeneration via Targeting TRAF-6 and BCL-2 Proteins in Mouse Model of Osteoarthritis

Scientists screened rutin (RT) for its activity against osteoarthritis with in vivo and in vitro models of osteoarthritis. Pretreatment of RT decreased the ECM degradation, inhibited expression of TRAF-6 and BCL-2 the NF-κB/MAPK pathway proteins.
[Aging]
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Doxorubicin Suppresses Chondrocyte Differentiation by Stimulating ROS Production

Scientists explored the specific mechanism of doxorubicin in osteoarthritis (OA). A mouse OA model was established by destabilizing the medial meniscus, and the role of DOX was determined by intraperitoneally injecting five or ten mg/kg DOX.
[European Journal of Pharmaceutical Sciences]
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SPRY4 Acts as an Indicator of Osteoarthritis Severity and Regulates Chondrocyte Hypertrophy and ECM Protease Expression

Using SPRY4 gene delivery into healthy and degenerated chondrocytes, investigators elucidated the role of SPRY4 in preventing chondrocyte hypertrophy. In addition to using the human cartilage tissues with the destabilization of the model in Sprague-Dawley rats, the role of SPRY4 in cartilage tissues and chondrocytes was explored through their molecular and histological analysis.
[NPJ Regenerative Medicine]
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The Chondrogenic Potential of First-Trimester and Term Placental Mesenchymal Stem/Stromal Cells

MSCs were isolated from first-trimester and term placentae. BM-MSCs were commercially obtained. Chondrogenesis was induced by micromass culture in commercial chondrogenic media for 7, 14, or 21 days.
[Cartilage]
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Long-Term Stability, High Strength, and 3D Printable Alginate Hydrogel for Cartilage Tissue Engineering Application

Researchers developed a double crosslinked alginate hydrogel for 3D bioprinting, and human umbilical cord MSCs could differentiate into chondrocytes on its printed 3D scaffold after four weeks’ culture.
[Biomedical Materials]
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