3D Printed Alginate Bead Generator for High-Throughput Cell Culture

Scientists designed a 3D printed bead generator that uses an airflow to cleave beads from a stream of hydrogel solution. They demonstrated that viability and biological function of chondrocytes were not adversely affected by high frequency using this bead generator.
[Biomedical Microdevices]
Lee, D., Greer, S. E., Kuss, M. A., An, Y., & Dudley, A. T. (2021). 3D printed alginate bead generator for high-throughput cell culture. Biomedical Microdevices, 23(2), 22. https://doi.org/10.1007/s10544-021-00561-4 Cite
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Ionizing Radiation Exposure of Stem Cell-Derived Chondrocytes Affects Their Gene and MicroRNA Expression Profiles and Cytokine Production

The authors evaluated the effect of ionizing radiation on chondrocyte-like cells differentiated from hiPSCs by determining their gene and microRNA expression profile and proteomic analysis.
[Scientific Reports]
Stelcer, E., Kulcenty, K., Rucinski, M., Kruszyna-Mochalska, M., Skrobala, A., Sobecka, A., Jopek, K., & Suchorska, W. M. (2021). Ionizing radiation exposure of stem cell-derived chondrocytes affects their gene and microRNA expression profiles and cytokine production. Scientific Reports, 11(1), 7481. https://doi.org/10.1038/s41598-021-86230-1 Cite
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Assessment of the Neuroprotective and Stemness Properties of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells under Variable (5% vs. 21%) Aerobic Conditions

Scientists investigated ten different properties of these cells cultured under 21% and 5% oxygen concentrations.
[Cells]
Tomecka, E., Lech, W., Zychowicz, M., Sarnowska, A., Murzyn, M., Oldak, T., Domanska-Janik, K., Buzanska, L., & Rozwadowska, N. (2021). Assessment of the Neuroprotective and Stemness Properties of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells under Variable (5% vs. 21%) Aerobic Conditions. Cells, 10(4), 717. https://doi.org/10.3390/cells10040717 Cite
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Mesenchymal Stem/Stromal Cells as a Valuable Source for the Treatment of Immune-Mediated Disorders

The authors discuss the therapeutic applications of mesenchymal stem/stromal cell (MSC) secretome and its biomedical aspects related to immune-mediated conditions. Sources for MSC extraction, their migration and homing properties, therapeutic molecules released by MSCs, and the pathways and molecular mechanisms possibly involved in the exceptional immunoregulatory competence of MSCs are discussed.
[Stem Cell Research & Therapy]
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An Integrated Analysis of the Competing Endogenous RNA Network and Co-Expression Network Revealed Seven Hub Long Non-Coding RNAs in Osteoarthritis

Investigators uncovered the hub long non-coding RNAs differentially expressed in osteoarthritis cartilage using an integrated analysis of the competing endogenous RNA network and co-expression network.
[Bone & Joint Research]
Chen, H., & Chen, L. (2020). An integrated analysis of the competing endogenous RNA network and co-expression network revealed seven hub long non-coding RNAs in osteoarthritis. Bone & Joint Research, 9(3), 90–98. https://doi.org/10.1302/2046-3758.93.BJR-2019-0140.R2 Cite
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Amniotic Membrane-Mesenchymal Stromal Cells Secreted Factors and Extracellular Vesicle-miRNAs: Anti-Inflammatory and Regenerative Features for Musculoskeletal Tissues

37 cytokines/chemokines were identified at varying abundance, some of which involved in both chemotaxis and homeostasis of inflammatory cells and in positive remodeling of extracellular matrix, often damaged in tendinopathy and osteoarthritis.
[Stem Cells Translational Medicine]
Ragni, E., Papait, A., Orfei, C. P., Silini, A. R., Colombini, A., Viganò, M., Libonati, F., Parolini, O., & Girolamo, L. de. (n.d.). Amniotic membrane-mesenchymal stromal cells secreted factors and extracellular vesicle-miRNAs: Anti-inflammatory and regenerative features for musculoskeletal tissues. STEM CELLS Translational Medicine, n/a(n/a). https://doi.org/https://doi.org/10.1002/sctm.20-0390 Cite
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Differentiation of Hypertrophic Chondrocytes from Human iPSCs for the In Vitro Modeling of Chondrodysplasias

The authors developed a three-dimensional culture protocol to differentiate hypertrophic chondrocytes from induced pluripotent stem cells (iPSCs) and examined the phenotype caused by MATN3 and COL10A1 mutations.
[Stem Cell Reports]
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Chondrogenesis of Human Amniotic Fluid Stem Cells in Chitosan-Xanthan Scaffold for Cartilage Tissue Engineering

Scientists investigated the chondrogenic potential of amniotic fluid stem cells in a micromass system under insulin-like growth factor 1 stimuli, as well as to look at their potential to differentiate directly when cultured in a porous chitosan-xanthan scaffold.
[Scientific Reports]
Zuliani, C. C., Damas, I. I., Andrade, K. C., Westin, C. B., Moraes, Â. M., & Coimbra, I. B. (2021). Chondrogenesis of human amniotic fluid stem cells in Chitosan-Xanthan scaffold for cartilage tissue engineering. Scientific Reports, 11(1), 3063. https://doi.org/10.1038/s41598-021-82341-x Cite
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Small Extracellular Vesicles from Adipose Derived Stromal Cells Significantly Attenuate In Vitro the Nf-κB Dependent Inflammatory/Catabolic Environment of Osteoarthritis

Researchers investigated the effects of small extracellular vesicles from adipose derived stromal cells on both chondrocytes and synoviocytes, in order to gain insights into the mechanisms modulating the inflammatory/catabolic osteoarthritis environment.
[Scientific Reports]
Cavallo, C., Merli, G., Borzì, R. M., Zini, N., D’Adamo, S., Guescini, M., Grigolo, B., Di Martino, A., Santi, S., & Filardo, G. (2021). Small Extracellular Vesicles from adipose derived stromal cells significantly attenuate in vitro the NF-κB dependent inflammatory/catabolic environment of osteoarthritis. Scientific Reports, 11(1), 1053. https://doi.org/10.1038/s41598-020-80032-7 Cite
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Single Cell Transcriptomic Analysis of Human Pluripotent Stem Cell Chondrogenesis

Scientists combined bulk RNA sequencing, single cell RNA sequencing, and bioinformatic analyses, including weighted gene co-expression analysis, to investigate the gene regulatory networks regulating human iPSC differentiation under chondrogenic conditions.
[Nature Communications]
Wu, C.-L., Dicks, A., Steward, N., Tang, R., Katz, D. B., Choi, Y.-R., & Guilak, F. (2021). Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis. Nature Communications, 12(1), 362. https://doi.org/10.1038/s41467-020-20598-y Cite
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Single-Cell Transcriptome Analysis of Uncultured Human Umbilical Cord Mesenchymal Stem Cells

Scientists investigated freshly isolated, uncultured umbilical cord (UC)-MSCs by single-cell RNA sequencing and found two populations of UC-MSCs.
[Stem Cell Research & Therapy]
Zhang, S., Wang, J. Y., Li, B., Yin, F., & Liu, H. (2021). Single-cell transcriptome analysis of uncultured human umbilical cord mesenchymal stem cells. Stem Cell Research & Therapy, 12(1), 25. https://doi.org/10.1186/s13287-020-02055-1 Cite
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