CRISPR-Cas9

[Scientific Reports] Scientists integrated genome-wide datasets of RNA-chromatin and promoter-genome interactions in human ESCs to identify putative RNA-mediated promoter interactions.

[Proceedings of the National Academy of Sciences of the United States of America] Scientists used desmoglein 3, a pemphigus antigen expressed in keratinocytes, to analyze peripheral tolerance under physiological antigen-expression conditions.

[Cancer Immunology Research] An epigenetic CRISPR-Cas9 screen of 1,218 chromatin regulators identified TRIM28 as a suppressor of PD-L1 expression.

[Cell Reports] Pharmacologic inhibition of CHK1 alone caused apoptotic growth suppression of both PDAC cell lines and organoids, which correlated with loss of MYC expression.

[Experimental and Molecular Medicine] Investigators showed that O-GlcNAcylation of Sox2 at threonine 258 regulated mouse ESC self-renewal and early cell fate.

[Journal of Experimental Medicine] Investigators described the discovery of an agonist of the nuclear receptor NR2F1 that specifically activated dormancy programs in malignant cells.