CRISPR-Cas9

[Stem Cell Reports] Scientists report successful targeting of the CCR5 locus in iPSCs generated from T cells or fibroblasts from Mauritian cynomolgus macaques, using CRISPR-Cas9 technology.

[Stem Cell Reports] Scientists performed molecular dynamic simulations to determine the effect of missense variant rs2229774 on retinoic acid receptor gamma (RARG) structure and then validated these predictions using CRISPR-Cas9-genome-edited, induced pluripotent stem cell-derived cardiomyocytes.

[Journal of Clinical Investigation] Through comparative chromatin state and gene expression analyses in ovarian CSCs vs. non-CSCs, researchers identified FOXK2 as a highly expressed stemness-specific transcription factor in ovarian cancer.

[Journal of Cancer Research and Clinical Oncology] Nox4 inactivation in normal HSCs and progenitor cells caused a minor reduction in HSC numbers and reconstitution capacity.

[Biomarker Research] Inactivation of BCL11B were performed by CRISPR/Cas9 in human T cells. Immunophenotypic and transcriptional profiles of sgBCL11B T cells were characterized by cytometer and transcriptomics, respectively.

[Journal of Experimental & Clinical Cancer Research] The authors provide a mechanistic discussion of autophagy in prostate cancer.