embryonic stem cells

[Stem Cell Research] Researchers employed non-homologous recombination and generated a mouse ESC line carrying a transgene expressing DNMT1 Y495C mutation in the wild-type background as a model for studying the abnormal neurogenesis due to this mutation.

[Stem Cell Research] To further understand the exact role of FLNC in dilated cardiomyopathy, scientists generated a human FLNC knockout cell line from the original ESC line H9 by CRISPR/Cas9 gene editing technology.

[Journal of Biological Chemistry] To better understand the role of TAF8 in TFIID, the authors investigated the requirement of the different regions of TAF8 for the in vitro assembly of lobe B and C, and the importance of certain TAF8 regions for mouse ESC viability.

[Stem Cell Research & Therapy] Scientists outline the particular approaches in the different published clinical trials for cell-based therapies for retinal diseases, and present the controversies surrounding experimental treatments offered outside of legitimate studies.

[Nature Communications] Investigators developed an approach to jointly profile the epigenome and transcriptome in the same single cell. Utilizing this method, they profiled H3K27me3 and H3K4me3 levels along with gene expression in mouse ESCs with localized Wnt3a signaling, revealing the cell type-specific maps of the epigenome and transcriptome in divided daughter cells.

[Molecular Cell] ESCs expressing a chimeric CBX7 were impaired in their ability to properly form embryoid bodies and neural progenitor cells and showed reduced activation of lineage-specific genes across differentiation.