Models of the Blood-Brain Barrier using iPSC-Derived Cells

The authors summarize important functions of the blood-brain barrier (BBB) and current efforts to create iPSC-derived BBB models in both static and dynamic conditions.
[Molecular and Cellular Neuroscience]
Delsing, L., Herland, A., Falk, A., Hicks, R., Synnergren, J., & Zetterberg, H. (2020). Models of the blood-brain barrier using iPSC-derived cells. Molecular and Cellular Neuroscience, 103533. https://doi.org/10.1016/j.mcn.2020.103533 Cite
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Robust Differentiation of Human Pluripotent Stem Cells Into Endothelial Cells via Temporal Modulation of ETV2 with Modified mRNA

Scientists describe a method for rapid, consistent, and highly efficient generation of human iPSC–derived endothelial cells. The protocol entailed the delivery of modified mRNA encoding the transcription factor ETV2 at the intermediate mesodermal stage of differentiation.
[Science Advances]
Wang, K., Lin, R.-Z., Hong, X., Ng, A. H., Lee, C. N., Neumeyer, J., Wang, G., Wang, X., Ma, M., Pu, W. T., Church, G. M., & Melero-Martin, J. M. (2020). Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA. Science Advances, 6(30), eaba7606. https://doi.org/10.1126/sciadv.aba7606 Cite
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Apoptotic Exosome-Like Vesicles Regulate Endothelial Gene Expression, Inflammatory Signaling, and Function through the NF-κB Signaling Pathway

Investigators evaluated the impact of apoptotic exosome-like vesicles on endothelial gene expression and function in the context of a pro-apoptotic stimulus.
[Scientific Reports]
Migneault, F., Dieudé, M., Turgeon, J., Beillevaire, D., Hardy, M.-P., Brodeur, A., Thibodeau, N., Perreault, C., & Hébert, M.-J. (2020). Apoptotic exosome-like vesicles regulate endothelial gene expression, inflammatory signaling, and function through the NF-κB signaling pathway. Scientific Reports, 10(1), 12562. https://doi.org/10.1038/s41598-020-69548-0 Cite
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The Lymphatic Vasculature in the 21st Century: Novel Functional Roles in Homeostasis and Disease

The molecular characterization of lymphatic vascular development and our understanding of this vasculature’s role in pathophysiological conditions has greatly improved in recent years, changing conventional views about the roles of the lymphatic vasculature in health and disease.
[Cell]
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SIRT7 Deficiency Suppresses Inflammation, Induces EndoMT, and Increases Vascular Permeability in Primary Pulmonary Endothelial Cells

Researchers investigated the effects of SIRT7 depletion on lipopolysaccharide-induced inflammatory responses and endothelial barrier permeability in human primary pulmonary endothelial cells.
[Scientific Reports]
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Role of METTL3-Dependent N6-Methyladenosine mRNA Modification in the Promotion of Angiogenesis

METTL3 silencing or METTL3 overexpression altered endothelial cell viability, proliferation, migration, and tube formation in vitro.
[Molecular Therapy]
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Beneficial Effects of IL-4 and IL-6 on Rat Neonatal Target Cardiac Cells

Scientists found that M2 compared to M1 macrophage-conditioned media upregulated neonatal cardiomyocyte proliferation, suppressed myofibroblast-induced differentiation and stimulated endothelial cell tube formation.
[Scientific Reports]
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Intrinsic Transcriptomic Sex Differences in Human Endothelial Cells at Birth and in Adults Are Associated with Coronary Artery Disease Targets

The authors analyzed endothelial cell (EC) gene expression data from boy–girl twins at birth and in non-twin adults to detect sex differences at different stages of life, and show that 14–25% of the EC transcriptome was sex-biased.
[Scientific Reports]
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Transcriptomic Comparison of Human and Mouse Brain Microvessels

Investigators performed RNA sequencing on brain microvessel preparations isolated from snap-frozen human and mouse tissues by laser capture microdissection
[Scientific Reports]
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SARS-CoV-2 Entry Receptor ACE2 Is Expressed on Very Small CD45 Precursors of Hematopoietic and Endothelial Cells and in Response to Virus Spike Protein Activates the Nlrp3 Inflammasome

Investigators demonstrated for the first time that ACE2 and the entry-facilitating transmembrane protease TMPRSS2 were expressed on very small CD133+CD34+LinCD45 cells in human umbilical cord blood, which could be specified into functional hematopoietic stem cells and endothelial progenitor cells.
[Stem Cell Reviews and Reports]
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Epigenetics in Modulating Immune Functions of Stromal and Immune Cells in the Tumor Microenvironment

The authors review recent studies demonstrating how specific epigenetic mechanisms drive aspects of stromal and immune cell differentiation with implications for the development of solid tumor therapeutics, focusing on the pancreatic ductal adenocarcinoma tumor microenvironment as a representative of solid tumors.
[Cellular & Molecular Immunology]
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Impact of Local Allo-Immunity and Recipient Cells in Transplant Arteriosclerosis

Investigators report the transcriptional profiling of 11,868 cells in a mouse model of transplant arteriosclerosis by single-cell RNA sequencing.
[Circulation Research]
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