Three-Dimensional Human Neural Stem Cell Models to Mimic Heparan Sulfate Proteoglycans and the Neural Niche

This review examines the current knowledge of heparan sulfate proteoglycans in the stem cell niche, human stem cell models, and their role in the development of 3D models that mimic the in vivo neural extracellular matrix.
[Seminars in Thrombosis and Hemostasis]
Peall, I. W., Okolicsanyi, R. K., Griffiths, L. R., & Haupt, L. M. (2021). Three-Dimensional Human Neural Stem Cell Models to Mimic Heparan Sulfate Proteoglycans and the Neural Niche. Seminars in Thrombosis and Hemostasis, 47(03), 308–315. https://doi.org/10.1055/s-0041-1724117 Cite
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Stiffening of the Extracellular Matrix Is a Sufficient Condition for Airway Hyperreactivity

The authors hypothesized that stiffening of the airway extracellular matrix is a core pathological change sufficient to support excessive bronchoconstriction even in the absence of inflammation.
[Journal of Applied Physiology]
Jamieson, R. R., Stasiak, S. E., Polio, S. R., Augspurg, R. D., McCormick, C. A., Ruberti, J. W., & Parameswaran, H. (2021). Stiffening of the Extracellular Matrix is a Sufficient Condition for Airway Hyperreactivity. Journal of Applied Physiology. https://doi.org/10.1152/japplphysiol.00554.2020 Cite
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Rosiglitazone Attenuates High Glucose-Induced Proliferation, Inflammation, Oxidative Stress and Extracellular Matrix Accumulation in Mouse Mesangial Cells through the Gm26917/miR-185-5p Pathway

Rosiglitazone significantly inhibited high glucose-induced proliferation, oxidative stress, inflammatory responses and extracellular matrix accumulation in mouse mesangial cells.
[Endocrine Journal]
Zhao, D., Guo, J., Liu, L., & Huang, Y. (2021). Rosiglitazone attenuates high glucose-induced proliferation, inflammation, oxidative stress and extracellular matrix accumulation in mouse mesangial cells through the Gm26917/miR-185-5p pathway. Endocrine Journal, advpub. https://doi.org/10.1507/endocrj.EJ20-0783 Cite
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Matrix Composition Modulates Vitamin D3’s Effects on 3D Collagen Fiber Organization by MCF10A Cells

The authors embedded MCF10A normal human breast epithelial cells in two different matrices – collagen type I, and collagen type I+10% Matrigel; treatment with calcitriol resulted in flatter epithelial structures.
[Tissue Engineering Part A]
Hasan, N., Zhang, Y., Georgakoudi, I., Sonnenschein, C., & Soto, A. M. (2021). Matrix composition modulates vitamin D3’s effects on 3D collagen fiber organization by MCF10A cells. Tissue Engineering Part A. https://doi.org/10.1089/ten.TEA.2020.0371 Cite
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Differential Reprogramming of Breast Cancer Subtypes in 3D Cultures and Implications for Sensitivity to Targeted Therapy

Researchers systematically compared the transcriptomes of these different culture conditions by RNAseq of 14 breast cancer cell lines cultured in both 2D and 3D conditions.
[Scientific Reports]
Koedoot, E., Wolters, L., Smid, M., Stoilov, P., Burger, G. A., Herpers, B., Yan, K., Price, L. S., Martens, J. W. M., Le Dévédec, S. E., & van de Water, B. (2021). Differential reprogramming of breast cancer subtypes in 3D cultures and implications for sensitivity to targeted therapy. Scientific Reports, 11(1), 7259. https://doi.org/10.1038/s41598-021-86664-7 Cite
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3D iPSC Modeling of the Retinal Pigment Epithelium-Choriocapillaris Complex Identifies Factors Involved in the Pathology of Macular Degeneration

By incorporating iPSC-derived cells into a hydrogel-based extracellular matrix, researchers developed a 3D retinal pigment epithelium (RPE)- choriocapillaris (CC) model that recapitulated key features of both healthy and age-related macular degeneration/macular dystrophie eyes and provides modular control over RPE and CC layers.
[Cell Stem Cell]
Manian, K. V., Galloway, C. A., Dalvi, S., Emanuel, A. A., Mereness, J. A., Black, W., Winschel, L., Soto, C., Li, Y., Song, Y., DeMaria, W., Kumar, A., Slukvin, I., Schwartz, M. P., Murphy, W. L., Anand-Apte, B., Chung, M., Benoit, D. S. W., & Singh, R. (2021). 3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration. Cell Stem Cell. https://doi.org/10.1016/j.stem.2021.02.006 Cite
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CD148 Deficiency in Fibroblasts Promotes the Development of Pulmonary Fibrosis

Scientists investigated the role of CD148 in the profibrotic phenotype of fibroblasts in idiopathic pulmonary fibrosis. Conditional CD148 fibroblast-specific knockout mice were generated and exposed to bleomycin, and then assessed for pulmonary fibrosis.
[American Journal of Respiratory and Critical Care Medicine]
Tsoyi, K., Liang, X., De Rossi, G., Ryter, S. W., Xiong, K., Chu, S. G., Liu, X., Ith, B., Celada, L. J., Romero, F., Robertson, M. J., Esposito, A. J., Poli, S., El-Chemaly, S., Perrella, M. A., Shi, Y., Whiteford, J., & Rosas, I. O. (2021). CD148 Deficiency in Fibroblasts Promotes the Development of Pulmonary Fibrosis. American Journal of Respiratory and Critical Care Medicine. https://doi.org/10.1164/rccm.202008-3100OC Cite
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Stem Cell-Based Approaches in Cardiac Tissue Engineering: Controlling the Microenvironment for Autologous Cells

The authors provide a detailed overview of the recent advances in microengineering of autologous stem cell-based tissue engineering platforms for the repair of damaged cardiac tissue.
[Biomedicine & Pharmacotherapy]
Augustine, R., Dan, P., Hasan, A., Khalaf, I. M., Prasad, P., Ghosal, K., Gentile, C., McClements, L., & Maureira, P. (2021). Stem cell-based approaches in cardiac tissue engineering: controlling the microenvironment for autologous cells. Biomedicine & Pharmacotherapy, 138, 111425. https://doi.org/10.1016/j.biopha.2021.111425 Cite
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Fibrotic Enzymes Modulate Wound‐Induced Skin Tumorigenesis

Researchers performed genome‐wide expression analysis comparing fibroblasts in normal, inflammatory and tumor‐associated skin. Cancer‐associated fibroblasts exhibit a fibrotic gene signature in wound‐induced tumors, demonstrating persistent extracellular matrix remodeling within these tumors.
[EMBO Reports]
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Depletion of Arg/Abl2 Improves Endothelial Cell Adhesion and Prevents Vascular Leak during Inflammation

Scientists identified the kinase Arg/Abl2 as a mediator of endothelial barrier disruption, but the role of Arg in endothelial monolayer regulation and its relevance in vivo remained poorly understood.
[Angiogenesis]
Amado-Azevedo, J., van Stalborch, A.-M. D., Valent, E. T., Nawaz, K., van Bezu, J., Eringa, E. C., Hoevenaars, F. P. M., De Cuyper, I. M., Hordijk, P. L., van Hinsbergh, V. W. M., van Nieuw Amerongen, G. P., Aman, J., & Margadant, C. (2021). Depletion of Arg/Abl2 improves endothelial cell adhesion and prevents vascular leak during inflammation. Angiogenesis. https://doi.org/10.1007/s10456-021-09781-x Cite
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Restoration of the Healing Microenvironment in Diabetic Wounds with Matrix-Binding IL-1 Receptor Antagonist

Researchers demonstrated that matrix-binding IL-1Ra improves wound healing in diabetic mice by re-establishing a pro-healing microenvironment characterised by lower levels of pro-inflammatory cells, cytokines and senescent fibroblasts, and higher levels of anti-inflammatory cytokines and growth factors.
[Communications Biology]
Tan, J. L., Lash, B., Karami, R., Nayer, B., Lu, Y.-Z., Piotto, C., Julier, Z., & Martino, M. M. (2021). Restoration of the healing microenvironment in diabetic wounds with matrix-binding IL-1 receptor antagonist. Communications Biology, 4(1), 1–11. https://doi.org/10.1038/s42003-021-01913-9 Cite
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