flow cytometry

[Anti-Cancer Drugs] Subcutaneous A549 lung cancer and murine Lewis lung carcinoma (LLC) models were constructed, which were further intravenously injected with platinum-based drugs or concomitant administrated with anti-PD-1 antibody and or anti-NKG2D antibody.

[International Journal of Biochemistry & Cell Biology] Researchers explored the effects of metformin on the osteogenesis, adipogenesis and angiogenesis of human umbilical cord-MSCs, and attempted to explain the molecular fluctuations of metformin through the mapping of protein profiles.

[World Journal of Microbiology & Biotechnology] Scientists explored the anticancer activity and its underlying mechanisms of PPT in HCT116 cells. The MTT assay was used to monitor the cell viability.

[European Journal of Pharmaceutical Sciences] Scientists explored the specific mechanism of doxorubicin in osteoarthritis (OA). A mouse OA model was established by destabilizing the medial meniscus, and the role of DOX was determined by intraperitoneally injecting five or ten mg/kg DOX.

[Scientific Reports] Researchers explored the relationship between PAX3-FOXO1 and B7-H3 and the biological roles of B7-H3 in alveolar rhabdomyosarcoma. Quantitative real time PCR and flow cytometry revealed that PAX3-FOXO1 knockdown downregulated B7-H3 expression in all the selected cell lines, suggesting that PAX3-FOXO1 positively regulates B7-H3 expression.

[International Archives of Allergy and Immunology] Human umbilical cord MSC-derived exosome dramatically inhibited Th2 differentiation, decreased GATA binding protein-3 protein expressions and IL-4 levels by about two times in CD4+ T cells.