flow cytometry

[Laboratory Investigation] The experiments used a novel flow cytometry assay to quantify depolarization of synaptosomes by KCl after loading with FM2–10, which induced a fluorescence reduction associated with synaptic vesicle release; the degree of reduction in cryopreserved human samples equaled that seen in fresh mouse synaptosomes.

[Naunyn-Schmiedebergs Archives of Pharmacology] Scientists investigated the effect of diclofenac, a selective COX-2 inhibitor, on cell adhesion molecules and apoptosis in human colon adenocarcinoma cells. Levels of homing cell adhesion molecule, intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and epithelial cell adhesion molecule were evaluated in cancer cells overexpressing or not expressing COX-2.

[Acta Biochimica et Biophysica Sinica] Researchers investigated the relationship between miR-144-3p and CEP55 expression. They evaluated the association between miR-144-3p and CEP55 expression and proliferation, invasion and apoptosis of non-small cell lung cancer cells.

[International Archives of Allergy and Immunology] Cough-variant asthma (CVA) is a special type of asthma, solely manifesting with coughing. Investigators suggested that airway inflammation was associated with CVA pathogenesis.

[Cell Death Discovery] Scientists investigated the regulatory effects of Reelin on multiple myeloma and its underlying pathogenic mechanisms. In vitro studies showed that Reelin depletion regulated osteolysis and osteogenesis balance, cell cycle, invasiveness, and apoptosis in SP2/0 cells.

[Cell Reports] By studying physiological homing of fetal hematopoietic stem and progenitor cells (HSPCs), scientists showed the critical requirement of balanced local crosstalk within the skeletal niche for successful HSPC settlement in bone marrow.