flow cytometry

[Scientific Reports] The authors reported a phenotypic screening of a human secretome library in human Treg cells utilising a high throughput flow cytometry technology.

[Photodermatology Photoimmunology & Photomedicine] Scientists analyzed whether ultraviolet A aggravated the particulate matter-induced inflammatory cascade, which contributed to the aging of skin-derived cells.

[Stem Cell Research & Therapy] Human monocytes were educated by exosomes from lipopolysaccharide-primed mesenchymal stem cells (MSCs) and compared to monocytes educated by unprimed MSCs and uneducated monocytes to assess survival and clinical improvement in a xenogeneic mouse model of acute radiation syndrome.

[Scientific Reports] Investigators showed that the long-noncoding RNA colorectal neoplasia differentially expressed (CRNDE) was overexpressed in both acute myeloid leukemia samples and cell lines. CRNDE silencing inhibited proliferation and increased apoptotic rate and cell cycle arrest of KG-1a cells.

[Leukemia & Lymphoma] The authors investigated the prognostic significance of T cell immunoglobulin and mucin domain-containing protein 3 (TIM-3) in t(8;21) acute myeloid leukemia (AML). A total of 47 t(8;21) AML patients were tested for TIM-3 expression by multi-parameter flow cytometry at diagnosis.

[Acta Histochemica] Researchers evaluated platinum-based response in brain tumor U-87 MG, LN-18, and KELLY cell lines cultured in monolayer and neurosphere models. They evaluated mRNA expression of heat shock proteins and DNA repair gene ERCC1.