gene knockout

[Stem Cell Reviews and Reports] The presence of artificial introns, particularly an intron that carried an enhancer element, significantly increased the reporter expression levels in iPSCs compared to the intron-deleted control.

[Biochemical Pharmacology] The authors explained the incidence of hand-foot skin reaction in patients treated with tyrosine kinase inhibitors by focusing on keratinocyte toxicity and inhibition of vascular endothelial growth factor receptor, which played an essential role in angiogenesis.

[Stem Cell Research & Therapy] Bone-associated MSCs (BA-MSCs) were isolated from gene knockout mice, and the effect of Caspase-3/NLRP3 deficient BA-MSCs on hematopoiesis regulation was explored in vivo and ex vivo.

[Scientific Reports] Differentiation platforms were established to model neural induction from stem cells, and scientists characterized these differentiated cell types by 10x single cell RNA sequencing.

[Nature Communications] Neural-stem cells were isolated from parthenogenetic lizard embryos, rendered unresponsive to Hedgehog signaling via CRISPR/Cas9 gene knockout of smoothened, and implanted back into clonally-identical adults to regulate tail regeneration.

[eLife] Using a newly derived carcinogen transformed human cell model, researchers demonstrated that fine-tuned Drp1 repression primed a slow cycling 'stem/progenitor-like state', which was characterized by small networks of fused mitochondria and a gene-expression profile with elevated functional stem/progenitor markers and their regulators.