Single-Cell Chromatin Accessibility Profiling of Glioblastoma Identifies an Invasive Cancer Stem Cell Population Associated with Lower Survival

Researchers showed, using single-cell chromatin accessibility, that primary human glioblastomas (GBMs) harbored a heterogeneous self-renewing population whose diversity was captured in patient-derived GBMs stem cells.
[eLife]
Guilhamon, P., Chesnelong, C., Kushida, M. M., Nikolic, A., Singhal, D., MacLeod, G., Madani Tonekaboni, S. A., Cavalli, F. M., Arlidge, C., Rajakulendran, N., Rastegar, N., Hao, X., Hassam, R., Smith, L. J., Whetstone, H., Coutinho, F. J., Nadorp, B., Ellestad, K. I., Luchman, A. H., … Lupien, M. (2021). Single-cell chromatin accessibility profiling of glioblastoma identifies an Invasive cancer stem cell population associated with lower survival. ELife, 10, e64090. https://doi.org/10.7554/eLife.64090 Cite
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Inhibition of Gli2 Suppresses Tumorigenicity in Glioblastoma Stem Cells Derived from a De Novo Murine Brain Cancer Model

The Sleeping-Beauty transposon-induced glioblastoma model was used in leucine-rich repeat-containing G-protein coupled receptor 5-GFP knock-in mice identify GFP-positive cells in neurosphere cultures from mouse glioblastoma tissues
[Cancer Gene Therapy]
Tanigawa, S., Fujita, M., Moyama, C., Ando, S., Ii, H., Kojima, Y., Fujishita, T., Aoki, M., Takeuchi, H., Yamanaka, T., Takahashi, Y., Hashimoto, N., & Nakata, S. (2021). Inhibition of Gli2 suppresses tumorigenicity in glioblastoma stem cells derived from a de novo murine brain cancer model. Cancer Gene Therapy, 1–14. https://doi.org/10.1038/s41417-020-00282-5 Cite
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Gradient of Developmental and Injury Response Transcriptional States Defines Functional Vulnerabilities Underpinning Glioblastoma Heterogeneity

The authors performed single-cell RNA sequencing on >69,000 glioblastoma stem cells (GSCs) cultured from the tumors of 26 patients. They observed a high degree of inter- and intra-GSC transcriptional heterogeneity that could not be fully explained by DNA somatic alterations.
[Nature Cancer]
Richards, L. M., Whitley, O. K. N., MacLeod, G., Cavalli, F. M. G., Coutinho, F. J., Jaramillo, J. E., Svergun, N., Riverin, M., Croucher, D. C., Kushida, M., Yu, K., Guilhamon, P., Rastegar, N., Ahmadi, M., Bhatti, J. K., Bozek, D. A., Li, N., Lee, L., Che, C., … Pugh, T. J. (2021). Gradient of Developmental and Injury Response transcriptional states defines functional vulnerabilities underpinning glioblastoma heterogeneity. Nature Cancer, 1–17. https://doi.org/10.1038/s43018-020-00154-9 Cite
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PARK7 Maintains the Stemness of Glioblastoma Stem Cells by Stabilizing Epidermal Growth Factor Receptor Variant III

Deregulation of PARK7 has been implicated in the pathogenesis of various human diseases, including cancer. Scientists clarified the effect of PARK7 on stemness and radioresistance of glioblastoma stem cells.
[Oncogene]
Kim, J.-Y., Kim, H.-J., Jung, C.-W., Choi, B.-I., Lee, D.-H., & Park, M.-J. (2020). PARK7 maintains the stemness of glioblastoma stem cells by stabilizing epidermal growth factor receptor variant III. Oncogene, 1–14. https://doi.org/10.1038/s41388-020-01543-1 Cite
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The RNA m6A Reader YTHDF2 Maintains Oncogene Expression and Is a Targetable Dependency in Glioblastoma Stem Cells

Researchers interrogated N6-methyladenosine (m6A) mRNA modifications in glioma stem cells by methyl RNA-immunoprecipitation followed by sequencing and transcriptome analysis, finding transcripts marked by m6A often upregulated compared to normal neural stem cells.
[Cancer Discovery]
Dixit, D., Prager, B. C., Gimple, R. C., Poh, H. X., Wang, Y., Wu, Q., Qiu, Z., Kidwell, R. L., Kim, L. J. Y., Xie, Q., Vitting-Seerup, K., Bhargava, S., Dong, Z., Jiang, L., Zhu, Z., Hamerlik, P., Jaffrey, S. R., Zhao, J. C., Wang, X., & Rich, J. N. (2020). The RNA m6A reader YTHDF2 maintains oncogene expression and is a targetable dependency in glioblastoma stem cells. Cancer Discovery. https://doi.org/10.1158/2159-8290.CD-20-0331 Cite
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Molecular Analyses of Glioblastoma Stem-Like Cells and Glioblastoma Tissue

Researchers utilized both SNP array and gene expression profiling to better understand glioblastoma stem-like cells and their relation to malignant disease.
[PLoS One]
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ARS2/MAGL Signaling in Glioblastoma Stem Cells Promotes Self-Renewal and M2-Like Polarization of Tumor-Associated Macrophages

Investigators demonstrated that arsenite-resistance protein 2 (ARS2), a zinc finger protein that is essential for early mammalian development, played critical roles in glioblastoma stem cell maintenance and M2-like tumor-associated macrophage polarization.
[Nature Communications]
Yin, J., Kim, S. S., Choi, E., Oh, Y. T., Lin, W., Kim, T.-H., Sa, J. K., Hong, J. H., Park, S. H., Kwon, H. J., Jin, X., You, Y., Kim, J. H., Kim, H., Son, J., Lee, J., Nam, D.-H., Choi, K. S., Shi, B., … Park, J. B. (2020). ARS2/MAGL signaling in glioblastoma stem cells promotes self-renewal and M2-like polarization of tumor-associated macrophages. Nature Communications, 11(1), 2978. https://doi.org/10.1038/s41467-020-16789-2 Cite
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