in vitro

[British Journal of Cancer] From head-and-neck squamous cell carcinoma cell lines not expressing CYP1B1, investigators generated isogenic derivatives expressing the two forms. Proliferation, invasiveness, stem cell characteristics, sensitivity to anticancer agents and transcriptome were analyzed.

[Marine Drugs] A fucose-rich fucoidan was purified from brown seaweed Saccharina japonica, of which the UVB protective effect was investigated in vitro in keratinocytes of HaCaT cells and in vivo in zebrafish. The intracellular ROS levels and the viability of UVB-irradiated HaCaT cells were determined.

[Cell Reports] Researchers combined lineage tracing in a spontaneous metastatic mouse model of melanoma, advanced imaging, and single-cell RNA sequencing approaches to search for pathophysiologically relevant melanoma cellular states.

[Signal Transduction and Targeted Therapy] Scientists demonstrated that PTEN was a direct target of miR-410 by using bioinformatic tools and dual-luciferase reporter assays, and the miR-410-induced epithelial–mesenchymal transition and radioresistance were reversed by PI3K, Akt, and mTOR inhibitors or by restoring the expression of PTEN in non-small cell lung cancer cells.

[Current Opinion in Cell Biology] The authors summarized key aspects of mammary gland biology and corresponding organoid models. The investigators also discussed emerging applications where organoids will be transformative for studying both the biology and diseases of the breast.

[Nature] The authors showed that human ESCs can be used to generate gastruloids—three-dimensional multicellular aggregates that differentiate to form derivatives of the three germ layers organized spatiotemporally, without additional extra-embryonic tissues.