Cortical Neurons Derived from Human Pluripotent Stem Cells Lacking FMRP Display Altered Spontaneous Firing Patterns

Electrophysiological whole-cell voltage- and current-clamp recordings were performed on two control and three fragile X syndrome patient lines of human cortical neurons derived from iPSCs.
[Molecular Autism]
Full Article
Bookmark

No account yet? Register

0
Share

Genome Engineering of Induced Pluripotent Stem Cells to Manufacture Natural Killer Cell Therapies

The authors highlight the current sources for natural killer (NK) therapies and their respective constraints, discuss recent developments in the manufacturing and genetic engineering of iPSC-NK cells, and provide an overview of ongoing clinical trials using NK cells.
[Stem Cell Research & Therapy]
Genome engineering of induced pluripotent stem cells to manufacture natural killer cell therapies | Stem Cell Research & Therapy | Full Text. (n.d.). Retrieved June 23, 2020, from https://stemcellres.biomedcentral.com/articles/10.1186/s13287-020-01741-4 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Century Therapeutics Announces Acquisition of Empirica Therapeutics

Century Therapeutics has announced its acquisition of Empirica Therapeutics to leverage its iPSC-derived allogeneic cell therapies against glioblastoma.
[Businesswire (Century Therapeutics)]
Century Therapeutics Announces Acquisition of Empirica Therapeutics. (2020, June 23). https://www.businesswire.com/news/home/20200623005319/en/Century-Therapeutics-Announces-Acquisition-Empirica-Therapeutics Cite
Press Release
Bookmark

No account yet? Register

0
Share

Signaling Inhibitors Accelerate the Conversion of Mouse iPS Cells into Cancer Stem Cells in the Tumor Microenvironment

Inhibition of Erk1/2, tyrosine kinase, and/or GSK-3β was implied to be involved in the enhancement of the PI3K-AKT signaling pathway in the undifferentiated cells, resulting in the sustained stemness, and subsequent conversion of mouse iPSCs into cancer stem cells in the tumor microenvironment.
[Scientific Reports]
Du, J., Xu, Y., Sasada, S., Oo, A. K. K., Hassan, G., Mahmud, H., Khayrani, A. C., Alam, M. J., Kumon, K., Uesaki, R., Afify, S. M., Mansour, H. M., Nair, N., Zahra, M. H., Seno, A., Okada, N., Chen, L., Yan, T., & Seno, M. (2020). Signaling Inhibitors Accelerate the Conversion of mouse iPS Cells into Cancer Stem Cells in the Tumor Microenvironment. Scientific Reports, 10(1), 9955. https://doi.org/10.1038/s41598-020-66471-2 Cite
Full Article
Bookmark

No account yet? Register

0
Share

In Vitro Differentiated Human Stem Cell-Derived Neurons Reproduce Synaptic Synchronicity Arising during Neurodevelopment

Scientists generated human iPSC-derived neurons exhibiting spontaneous oscillatory activity after cultivation of up to six months, which resembled early oscillations observed in rodent neurons. This behavior was found in neurons generated using a more “native” embryoid body protocol, in contrast to a “fast” protocol based on NGN2 overexpression.
[Stem Cell Reports]
Rosa, F., Dhingra, A., Uysal, B., Mendis, G. D. C., Loeffler, H., Elsen, G., Mueller, S., Schwarz, N., Castillo-Lizardo, M., Cuddy, C., Becker, F., Heutink, P., Reid, C. A., Petrou, S., Lerche, H., & Maljevic, S. (2020). In Vitro Differentiated Human Stem Cell-Derived Neurons Reproduce Synaptic Synchronicity Arising during Neurodevelopment. Stem Cell Reports, 0(0). https://doi.org/10.1016/j.stemcr.2020.05.015 Cite
Full ArticleGraphical Abstract
Bookmark

No account yet? Register

0
Share

A Simple, Quick, and Efficient CRISPR/Cas9 Genome Editing Method for Human Induced Pluripotent Stem Cells

The authors report an electroporation-mediated plasmid CRISPR/Cas9 delivery approach for genome editing in iPSCs. With this approach, an edited iPSC cell line could be obtained within two weeks.
[Acta Pharmacologica Sinica]
Abstract
Bookmark

No account yet? Register

0
Share

Tooth Formation: Are the Hardest Tissues of the Human Body Hard to Regenerate?

Approaches for whole-tooth regeneration utilizing adult stem cells, iPSCs, or tooth germ cells transplantation are emerging as promising alternatives to overcome existing in vitro tissue generation hurdles. The authors discuss the most recent advances in cellular signaling guiding dental tissue genesis.
[International Journal of Molecular Sciences]
AbstractGraphical Abstract
Bookmark

No account yet? Register

0
Share

Autism-Associated SHANK3 Mutations Impair Maturation of Neuromuscular Junctions and Striated Muscles

Investigators used a combination of patient-derived human iPSCs, Shank3Δ11(−/−) mice, and Phelan-McDermid syndrome muscle biopsies from patients of different ages to analyze the role of SHANK3 on motor unit development.
[Science Translational Medicine]
Autism-associated SHANK3 mutations impair maturation of neuromuscular junctions and striated muscles | Science Translational Medicine. (n.d.). Retrieved June 17, 2020, from https://stm.sciencemag.org/content/12/547/eaaz3267 Cite
Full Article
Bookmark

No account yet? Register

0
Share

A lncRNA-miRNA-mRNA Network for Human Primed, Naive and Extended Pluripotent Stem Cells

Researchers successfully converted three in-house-derived primed human PSC lines (H10, H24, and iPS) to a naive state and an expanded pluripotent stem cell (EPS) state. Primed, naive and EPS cells displayed state-specific morphologies and expressed pluripotent markers.
[PLoS One]
Ma, Z., Li, Y., Zhang, Y., Chen, J., Tan, T., & Fan, Y. (2020). A lncRNA-miRNA-mRNA network for human primed, naive and extended pluripotent stem cells. PLOS ONE, 15(6), e0234628. https://doi.org/10.1371/journal.pone.0234628 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Critical Roles of Translation Initiation and RNA Uridylation in Endogenous Retroviral Expression and Neural Differentiation in Pluripotent Stem Cells

Scientists generated a conditional knockout of NAT1 in primed pluripotent stem cells (PSCs) and used the cells for the functional analyses of NAT1. Their results showed that NAT1 was required for the self-renewal and neural differentiation of primed PSCs.
[Cell Reports]
Full ArticleGraphical Abstract
Bookmark

No account yet? Register

0
Share

Cryopreserved Human Oocytes and Cord Blood Cells Can Produce Somatic Cell Nuclear Transfer-Derived Pluripotent Stem Cells with a Homozygous HLA Type

Researchers applied a modified derivation method to the PSC line and first obtained human somatic cell nuclear transfer-PSC lines derived from both donated cryopreserved oocytes and cord blood cells with a homozygous human leukocyte antigen type.
[Stem Cell Reports]
Full Article
Bookmark

No account yet? Register

0
Share
Share