The authors developed a human induced pluripotent stem cell-based model of isogenic bile salt export pump-deficient hepatocytes in a Transwell culture system. Induced hepatocytes exhibited defects in the apical export of bile acids but maintained a low intracellular bile acid concentration by inducing basolateral export.
[Stem Cell Reports]
6445212 LKCDLJKT items 1 apa default asc 1
Hayashi, H., Osaka, S., Sakabe, K., Fukami, A., Kishimoto, E., Aihara, E., Sabu, Y., Mizutani, A., Kusuhara, H., Naritaka, N., Zhang, W., Huppert, S. S., Sakabe, M., Nakamura, T., Hu, Y.-C., Mayhew, C., Setchell, K., Takebe, T., & Asai, A. (2021). Modeling Human Bile Acid Transport and Synthesis in Stem Cell-Derived Hepatocytes with a Patient-Specific Mutation. Stem Cell Reports, 0(0). https://doi.org/10.1016/j.stemcr.2020.12.008 Cite
Investigators developed a simple culture protocol under which the adult murine inguinal adipose tissue-derived stromal vascular fraction (Ad-SVF) reproducibly transdifferentiates into beating cardiomyocyte (CMs) without induction. The beating CMs showed the striated ventricular phenotype of cardiomyocytes and synchronised oscillation of the intracellular calcium concentration among cells on day 28 of Ad-SVF primary culture.
1254621 BBFC2KK8 items 1 apa default asc 1
Hyaluronan synthase 2 was strongly expressed in endothelial progenitor cells in the thickened intima. Moyamoya disease lesions showed minimal staining for chondroitin sulfate and hyaluronan in the endothelium, in contrast to control endothelium showing positive staining for both.
6445212 UKK8RNME items 1 apa default asc 1
Matsuo, M., Nadanaka, S., Soga, M., Sugiyama, T., Serigano, S., Shimano, K., Ichinose, F., Nakamura, T., Maeda, T., Houkin, K., Era, T., & Kitagawa, H. (2021). Vulnerability to shear stress caused by altered peri-endothelial matrix is a key feature of Moyamoya disease. Scientific Reports, 11(1), 1552. https://doi.org/10.1038/s41598-021-81282-9 Cite
Myogenesis in human induced pluripotent stem cells with the Tet/ON-myogenic differentiation 1 gene was induced by culturing them in doxycycline-supplemented differentiation medium for seven days.
1254621 UA63RZ5D items 1 apa default asc 1
Iwasaki, H., Ichihara, Y., Morino, K., Lemecha, M., Sugawara, L., Sawano, T., Miake, J., Sakurai, H., Nishi, E., Maegawa, H., & Imamura, T. (2021). MicroRNA-494-3p inhibits formation of fast oxidative muscle fibres by targeting E1A-binding protein p300 in human-induced pluripotent stem cells. Scientific Reports, 11(1), 1161. https://doi.org/10.1038/s41598-020-80742-y Cite
RheinCell Therapeutics GmbH announced it has received Good Manufacturing Practice certification and Manufacturing Authorization. This marks a landmark achievement for RheinCell, which is now among a select few iPSCs manufacturers to have received the critical certification.
6630899 nan items 1 apa default asc 1
Researchers present a computational workflow that could identify transcription factors that were crucial for modulating pathways involved in cell lineage specification.
[Cell Death & Disease]
6445212 3IYDWESD items 1 apa default asc 1
De Smedt, J., van Os, E. A., Talon, I., Ghosh, S., Toprakhisar, B., Furtado Madeiro Da Costa, R., Zaunz, S., Vazquez, M. A., Boon, R., Baatsen, P., Smout, A., Verhulst, S., van Grunsven, L. A., & Verfaillie, C. M. (2021). PU.1 drives specification of pluripotent stem cell-derived endothelial cells to LSEC-like cells. Cell Death & Disease, 12(1), 1–18. https://doi.org/10.1038/s41419-020-03356-2 Cite
Investigators introduce the development of induced pluripotent stem cells (iPSCs), the currently established iPSCs disease models, and artificial organoids related to neurodevelopmental impairments.
[International Journal of Medical Sciences]
The authors report that FMRP deficiency resulted in hyperactivated nonsense-mediated mRNA decay in human SH-SY5Y neuroblastoma cells and fragile X syndrome fibroblast-derived iPSCs.
[Nature Cell Biology]
6630899 QNRYCFAR items 1 apa default asc 1
Researchers showed that a recently identified autophagy enhancer (MSL-7) reduces human IAPP oligomer accumulation in human iPSC-derived β-cells and diminishes oligomer-mediated apoptosis of β-cells.
6630899 U3SDHJKZ items 1 apa default asc 1
Kim, J., Park, K., Kim, M. J., Lim, H., Kim, K. H., Kim, S.-W., Lee, E.-S., Kim, H. (Henry), Kim, S. J., Hur, K. Y., Kim, J. H., Ahn, J. H., Yoon, K.-H., Kim, J.-W., & Lee, M.-S. (2021). An autophagy enhancer ameliorates diabetes of human IAPP -transgenic mice through clearance of amyloidogenic oligomer. Nature Communications, 12(1), 183. https://doi.org/10.1038/s41467-020-20454-z Cite
Scientists advocate for a paradigm shift towards the application of human iPSC-derived systems for Alzheimer’s and Parkinson’s disease modeling and the development of improved human-based models in a dish for drug discovery and preclinical assessment of therapeutic targets.
6630899 WKJFSCEU items 1 apa default asc 1
The authors examine potential mechanisms for maintenance or shifts in heteroplasmy that occur in iPSCs generated by cellular reprogramming, and further discuss manipulations that can alter heteroplasmy to impact stem and differentiated cell performance.
[Trends in Cell Biology]