Models of the Blood-Brain Barrier using iPSC-Derived Cells

The authors summarize important functions of the blood-brain barrier (BBB) and current efforts to create iPSC-derived BBB models in both static and dynamic conditions.
[Molecular and Cellular Neuroscience]
Delsing, L., Herland, A., Falk, A., Hicks, R., Synnergren, J., & Zetterberg, H. (2020). Models of the blood-brain barrier using iPSC-derived cells. Molecular and Cellular Neuroscience, 103533. https://doi.org/10.1016/j.mcn.2020.103533 Cite
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Mapping Gene Circuits Essential for Germ Layer Differentiation via Loss-of-Function Screens in Haploid Human Embryonic Stem Cells

Scientists mapped the essential genes for the differentiation of human PSCs into the three germ layers by using a genome-wide loss-of-function library established in haploid human PSCs.
[Cell Stem Cell]
Yilmaz, A., Braverman-Gross, C., Bialer-Tsypin, A., Peretz, M., & Benvenisty, N. (2020). Mapping Gene Circuits Essential for Germ Layer Differentiation via Loss-of-Function Screens in Haploid Human Embryonic Stem Cells. Cell Stem Cell, 0(0). https://doi.org/10.1016/j.stem.2020.06.023 Cite
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Hematopoietic Stem Cells from Pluripotent Stem Cells: Clinical Potential, Challenges, and Future Perspectives

Ex vivo hematopoietic stem cell derivation from renewable sources such as induced pluripotent stem cells offers an experimental tool for studying developmental hematopoiesis, disease modeling, and drug discovery, and yields tremendous therapeutic potential for malignant and nonmalignant hematological disorders.
[Stem Cells Translational Medicine]
Demirci, S., Leonard, A., & Tisdale, J. F. (n.d.). Hematopoietic stem cells from pluripotent stem cells: Clinical potential, challenges, and future perspectives. STEM CELLS Translational Medicine, n/a(n/a). https://doi.org/10.1002/sctm.20-0247 Cite
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Taming Human Brain Organoids One Cell at a Time

The authors summarize recent approaches, progresses and challenges in resolving brain organoid’s complexity through single-cell transcriptomics.
[Seminars in Cell & Developmental Biology]
Atamian, A., Cordón-Barris, L., & Quadrato, G. (2020). Taming human brain organoids one cell at a time. Seminars in Cell & Developmental Biology. https://doi.org/10.1016/j.semcdb.2020.05.022 Cite
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Heart Regeneration Using Pluripotent Stem Cells

In this review, the authors summarize the updated knowledge, perspectives, and limitations of PSC-derived cardiomyocytes for heart regeneration.
[Journal of Cardiology]
Kadota, S., Tanaka, Y., & Shiba, Y. (2020). Heart regeneration using pluripotent stem cells. Journal of Cardiology, 0(0). https://doi.org/10.1016/j.jjcc.2020.03.013 Cite
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Induced Pluripotent Stem Cell-Derived Natural Killer Cells Gene-Modified to Express Chimeric Antigen Receptor-Targeting Solid Tumors

Scientists describe the characteristics of natural killer (NK) cells from multiple cell sources, including PSCs, the chimeric antigen receptor (CAR)-modification method and strategy for these NK cells.
[International Journal of Hematology]
Ueda, T., & Kaneko, S. (2020). Induced pluripotent stem cell-derived natural killer cells gene-modified to express chimeric antigen receptor-targeting solid tumors. International Journal of Hematology. https://doi.org/10.1007/s12185-020-02951-5 Cite
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Models of the Blood-Brain Barrier using iPSC-Derived Cells

The authors summarize important functions of the blood-brain barrier (BBB) and current efforts to create iPSC-derived BBB models in both static and dynamic conditions.
[Molecular and Cellular Neuroscience]
Delsing, L., Herland, A., Falk, A., Hicks, R., Synnergren, J., & Zetterberg, H. (2020). Models of the blood-brain barrier using iPSC-derived cells. Molecular and Cellular Neuroscience, 103533. https://doi.org/10.1016/j.mcn.2020.103533 Cite
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Developmental GABA Polarity Switch and Neuronal Plasticity in Bioengineered Neuronal Organoids

The authors report the directed self-organization of human induced pluripotent stem cells in a collagen hydrogel towards a highly interconnected neuronal network at a macroscale tissue format.
[Nature Communications]
Zafeiriou, M.-P., Bao, G., Hudson, J., Halder, R., Blenkle, A., Schreiber, M.-K., Fischer, A., Schild, D., & Zimmermann, W.-H. (2020). Developmental GABA polarity switch and neuronal plasticity in Bioengineered Neuronal Organoids. Nature Communications, 11(1), 3791. https://doi.org/10.1038/s41467-020-17521-w Cite
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Pluripotent Stem Cells Reveal Poly-Unsaturated Fatty Acids-provoked Mitochondrial Stress as a Central Node Potentiating Retinal Pigmented Epithelium Degeneration in Bietti’s Crystalline Dystrophy

Using CYP4V2 mutant PSCs as disease models, scientists demonstrated that retinal pigmented epithelial cells with CYP4V2 mutations presented a disrupted fatty acid homeostasis, which were characterized with excessive accumulation of poly-unsaturated fatty acid.
[Molecular Therapy]
Zhang, Z., Yan, B., Gao, F., Li, Q., Meng, X., Chen, P., Zhou, L., Deng, W., Li, C., Xu, W., Han, S., Feng, H., Li, Y., Chen, J., Yin, Z., Liao, C., Tse, H.-F., Xu, A., & Lian, Q. (2020). Pluripotent Stem Cells Reveal Poly-unsaturated Fatty Acids-provoked Mitochondrial Stress as a Central Node Potentiating Retinal Pigmented Epithelium Degeneration in Bietti’s Crystalline Dystrophy. Molecular Therapy, 0(0). https://doi.org/10.1016/j.ymthe.2020.07.024 Cite
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Robust Differentiation of Human Pluripotent Stem Cells Into Endothelial Cells via Temporal Modulation of ETV2 with Modified mRNA

Scientists describe a method for rapid, consistent, and highly efficient generation of human iPSC–derived endothelial cells. The protocol entailed the delivery of modified mRNA encoding the transcription factor ETV2 at the intermediate mesodermal stage of differentiation.
[Science Advances]
Wang, K., Lin, R.-Z., Hong, X., Ng, A. H., Lee, C. N., Neumeyer, J., Wang, G., Wang, X., Ma, M., Pu, W. T., Church, G. M., & Melero-Martin, J. M. (2020). Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA. Science Advances, 6(30), eaba7606. https://doi.org/10.1126/sciadv.aba7606 Cite
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Transient Dux Expression Facilitates Nuclear Transfer and Induced Pluripotent Stem Cell Reprogramming

Following the establishment of an inducible Dux transgenic mouse model, investigators demonstrated that transient overexpression of Dux not only improves somatic cell nuclear transfer efficiency but also increased that of chemically induced pluripotent stem cell reprogramming.
[EMBO Reports]
Yang, L., Liu, X., Song, L., Di, A., Su, G., Bai, C., Wei, Z., & Li, G. (2020). Transient Dux expression facilitates nuclear transfer and induced pluripotent stem cell reprogramming. EMBO Reports, n/a(n/a), e202050054. https://doi.org/10.15252/embr.202050054 Cite
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Genome-Scale In Vivo CRISPR Screen Identifies RNLS as a Target for Beta Cell Protection in Type 1 Diabetes

Using a genome-scale CRISPR screen in a mouse model for type 1 diabetes (T1D), researchers showed that deleting RNLS, a genome-wide association study candidate gene for T1D, made beta cells resistant to autoimmune killing.
[Nature Metabolism]
Cai, E. P., Ishikawa, Y., Zhang, W., Leite, N. C., Li, J., Hou, S., Kiaf, B., Hollister-Lock, J., Yilmaz, N. K., Schiffer, C. A., Melton, D. A., Kissler, S., & Yi, P. (2020). Genome-scale in vivo CRISPR screen identifies RNLS as a target for beta cell protection in type 1 diabetes. Nature Metabolism, 1–12. https://doi.org/10.1038/s42255-020-0254-1 Cite
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