Interferon-γ Signaling Synergizes with LRRK2 in Neurons and Microglia Derived from Human Induced Pluripotent Stem Cells

The authors employed dopaminergic neurons and microglia differentiated from patient-derived iPSCs carrying LRRK2 G2019S, the most common Parkinson’s disease-associated mutation.
[Nature Communications]
Interferon-γ signaling synergizes with LRRK2 in neurons and microglia derived from human induced pluripotent stem cells | Nature Communications. (n.d.). Retrieved October 14, 2020, from https://www.nature.com/articles/s41467-020-18755-4 Cite
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Single-Cell Transcriptomics of Parkinson’s Disease Human In Vitro Models Reveals Dopamine Neuron-Specific Stress Responses

Scientists performed the largest single-cell transcriptomic study of human iPSC-derived dopaminergic neurons to elucidate gene expression dynamics in response to cytotoxic and genetic stressors.
[Cell Reports]
Fernandes, H. J. R., Patikas, N., Foskolou, S., Field, S. F., Park, J.-E., Byrne, M. L., Bassett, A. R., & Metzakopian, E. (2020). Single-Cell Transcriptomics of Parkinson’s Disease Human In Vitro Models Reveals Dopamine Neuron-Specific Stress Responses. Cell Reports, 33(2). https://doi.org/10.1016/j.celrep.2020.108263 Cite
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Axol Biosciences Offers New Screening Assay for hiPSC-Derived Cells

Axol Biosciences has added multi-electrode array (MEA) screening services for human-iPSC-derived cells to its service offering for preclinical drug discovery. The company said that its new MEA screening services optimize hiPSC-derived cell culture, while also providing electrophysiology acquisition and analysis from physiologically relevant human cell models.
[The Science Advisory Board]
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3D Kidney Organoids for Bench-to-Bedside Translation

The authors propose that advancement in kidney organoid research will be facilitated through increasing knowledge on nephrogenesis and combining promising techniques such as organ-on-a-chip models.
[Journal of Molecular Medicine-Jmm]
Gupta✉, N., Dilmen, E., & Morizane, R. (2020). 3D kidney organoids for bench-to-bedside translation. Journal of Molecular Medicine. https://doi.org/10.1007/s00109-020-01983-y Cite
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SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF-Barrier in Human Brain Organoids

Researchers used human pluripotent stem cell-derived brain organoids to examine SARS-CoV-2 neurotropism. They found expression of viral receptor ACE2 in mature choroid plexus cells expressing abundant lipoproteins, but not in neurons or other cell types.
[Cell Stem Cell]
Pellegrini, L., Albecka, A., Mallery, D. L., Kellner, M. J., Paul, D., Carter, A. P., James, L. C., & Lancaster, M. A. (2020). SARS-CoV-2 infects the brain choroid plexus and disrupts the blood-CSF-barrier in human brain organoids. Cell Stem Cell, 0(0). https://doi.org/10.1016/j.stem.2020.10.001 Cite
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Hydrojet-Based Delivery of Footprint-Free iPSC-Derived Cardiomyocytes Into Porcine Myocardium

Scientists analyzed the applicability of a hydrojet-based method to deliver footprint-free iPSC-derived cardiomyocytes into the myocardium.
[Scientific Reports]
Weber, M., Fech, A., Jäger, L., Steinle, H., Bühler, L., Perl, R. M., Martirosian, P., Mehling, R., Sonanini, D., Aicher, W. K., Nikolaou, K., Schlensak, C., Enderle, M. D., Wendel, H. P., Linzenbold, W., & Avci-Adali, M. (2020). Hydrojet-based delivery of footprint-free iPSC-derived cardiomyocytes into porcine myocardium. Scientific Reports, 10(1), 16787. https://doi.org/10.1038/s41598-020-73693-x Cite
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Machine Learning Identifies Abnormal Ca2+ Transients in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Human-iPSC-derived cardiomyocytes (hiPSC-CMs) provide an excellent platform for potential clinical and research applications. Identifying abnormal Ca2+ transients is crucial for evaluating cardiomyocyte function. Investigators developed an analytical pipeline for automatic assessment of Ca2+ transient abnormality, by employing advanced machine learning methods together with an Analytical Algorithm.
[Scientific Reports]
Hwang, H., Liu, R., Maxwell, J. T., Yang, J., & Xu, C. (2020). Machine learning identifies abnormal Ca 2+ transients in human induced pluripotent stem cell-derived cardiomyocytes. Scientific Reports, 10(1), 16977. https://doi.org/10.1038/s41598-020-73801-x Cite
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Modeling Alcohol-Induced Neurotoxicity Using Human Induced Pluripotent Stem Cell-Derived Three-Dimensional Cerebral Organoids

Modeling binge drinking using iPSC-derived human cerebral organoids, scientists sought to quantify the downstream toxic effects of alcohol (ethanol) on neural pathology phenotypes and signaling pathways within the organoids.
[Translational Psychiatry]
Arzua, T., Yan, Y., Jiang, C., Logan, S., Allison, R. L., Wells, C., Kumar, S. N., Schäfer, R., & Bai, X. (2020). Modeling alcohol-induced neurotoxicity using human induced pluripotent stem cell-derived three-dimensional cerebral organoids. Translational Psychiatry, 10(1), 1–21. https://doi.org/10.1038/s41398-020-01029-4 Cite
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Human Cerebral Organoids Establish Subcortical Projections in the Mouse Brain after Transplantation

Researchers optimized a culturing protocol capable of efficiently generating small human cerebral organoids derived from hESCs and hiPSCs.
[Molecular Psychiatry]
Dong, X., Xu, S.-B., Chen, X., Tao, M., Tang, X.-Y., Fang, K.-H., Xu, M., Pan, Y., Chen, Y., He, S., & Liu, Y. (2020). Human cerebral organoids establish subcortical projections in the mouse brain after transplantation. Molecular Psychiatry, 1–13. https://doi.org/10.1038/s41380-020-00910-4 Cite
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Identification of a Dynamic Gene Regulatory Network Required for Pluripotency Factor-Induced Reprogramming of Mouse Fibroblasts and Hepatocytes

The authors investigated the early transcriptional events of cellular reprogramming triggered by the co‐expression of Oct4, Sox2, Klf4, and c‐Myc in mouse embryonic fibroblasts and mouse hepatocytes.
[EMBO Journal]
Papathanasiou, M., Tsiftsoglou, S. A., Polyzos, A. P., Papadopoulou, D., Valakos, D., Klagkou, E., Karagianni, P., Pliatska, M., Talianidis, I., Agelopoulos, M., & Thanos, D. (2020). Identification of a dynamic gene regulatory network required for pluripotency factor-induced reprogramming of mouse fibroblasts and hepatocytes. The EMBO Journal, n/a(n/a), e102236. https://doi.org/10.15252/embj.2019102236 Cite
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Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors

Scientists highlight the development of in vitro cell culture models that allow systematic studies of pancreatic cell mechanobiology in response to extracellular matrix proteins, biomechanical effects, soluble factor modulation of biomechanics, substrate stiffness, fluid flow and topography.
[Frontiers in Bioengineering and Biotechnology]
Tran, R., Moraes, C., & Hoesli, C. A. (2020). Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors. Frontiers in Bioengineering and Biotechnology, 8. https://doi.org/10.3389/fbioe.2020.583970 Cite
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