The authors employed dopaminergic neurons and microglia differentiated from patient-derived iPSCs carrying LRRK2 G2019S, the most common Parkinson’s disease-associated mutation.
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Interferon-γ signaling synergizes with LRRK2 in neurons and microglia derived from human induced pluripotent stem cells | Nature Communications. (n.d.). Retrieved October 14, 2020, from https://www.nature.com/articles/s41467-020-18755-4 Cite
Scientists performed the largest single-cell transcriptomic study of human iPSC-derived dopaminergic neurons to elucidate gene expression dynamics in response to cytotoxic and genetic stressors.
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Fernandes, H. J. R., Patikas, N., Foskolou, S., Field, S. F., Park, J.-E., Byrne, M. L., Bassett, A. R., & Metzakopian, E. (2020). Single-Cell Transcriptomics of Parkinson’s Disease Human In Vitro Models Reveals Dopamine Neuron-Specific Stress Responses. Cell Reports, 33(2). https://doi.org/10.1016/j.celrep.2020.108263 Cite
Axol Biosciences has added multi-electrode array (MEA) screening services for human-iPSC-derived cells to its service offering for preclinical drug discovery. The company said that its new MEA screening services optimize hiPSC-derived cell culture, while also providing electrophysiology acquisition and analysis from physiologically relevant human cell models.
[The Science Advisory Board]
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The authors propose that advancement in kidney organoid research will be facilitated through increasing knowledge on nephrogenesis and combining promising techniques such as organ-on-a-chip models.
[Journal of Molecular Medicine-Jmm]
Researchers used human pluripotent stem cell-derived brain organoids to examine SARS-CoV-2 neurotropism. They found expression of viral receptor ACE2 in mature choroid plexus cells expressing abundant lipoproteins, but not in neurons or other cell types.
[Cell Stem Cell]
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Pellegrini, L., Albecka, A., Mallery, D. L., Kellner, M. J., Paul, D., Carter, A. P., James, L. C., & Lancaster, M. A. (2020). SARS-CoV-2 infects the brain choroid plexus and disrupts the blood-CSF-barrier in human brain organoids. Cell Stem Cell, 0(0). https://doi.org/10.1016/j.stem.2020.10.001 Cite
Scientists analyzed the applicability of a hydrojet-based method to deliver footprint-free iPSC-derived cardiomyocytes into the myocardium.
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Weber, M., Fech, A., Jäger, L., Steinle, H., Bühler, L., Perl, R. M., Martirosian, P., Mehling, R., Sonanini, D., Aicher, W. K., Nikolaou, K., Schlensak, C., Enderle, M. D., Wendel, H. P., Linzenbold, W., & Avci-Adali, M. (2020). Hydrojet-based delivery of footprint-free iPSC-derived cardiomyocytes into porcine myocardium. Scientific Reports, 10(1), 16787. https://doi.org/10.1038/s41598-020-73693-x Cite
Human-iPSC-derived cardiomyocytes (hiPSC-CMs) provide an excellent platform for potential clinical and research applications. Identifying abnormal Ca2+ transients is crucial for evaluating cardiomyocyte function. Investigators developed an analytical pipeline for automatic assessment of Ca2+ transient abnormality, by employing advanced machine learning methods together with an Analytical Algorithm.
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Modeling binge drinking using iPSC-derived human cerebral organoids, scientists sought to quantify the downstream toxic effects of alcohol (ethanol) on neural pathology phenotypes and signaling pathways within the organoids.
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Arzua, T., Yan, Y., Jiang, C., Logan, S., Allison, R. L., Wells, C., Kumar, S. N., Schäfer, R., & Bai, X. (2020). Modeling alcohol-induced neurotoxicity using human induced pluripotent stem cell-derived three-dimensional cerebral organoids. Translational Psychiatry, 10(1), 1–21. https://doi.org/10.1038/s41398-020-01029-4 Cite
Researchers optimized a culturing protocol capable of efficiently generating small human cerebral organoids derived from hESCs and hiPSCs.
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The authors investigated the early transcriptional events of cellular reprogramming triggered by the co‐expression of Oct4, Sox2, Klf4, and c‐Myc in mouse embryonic fibroblasts and mouse hepatocytes.
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Papathanasiou, M., Tsiftsoglou, S. A., Polyzos, A. P., Papadopoulou, D., Valakos, D., Klagkou, E., Karagianni, P., Pliatska, M., Talianidis, I., Agelopoulos, M., & Thanos, D. (2020). Identification of a dynamic gene regulatory network required for pluripotency factor-induced reprogramming of mouse fibroblasts and hepatocytes. The EMBO Journal, n/a(n/a), e102236. https://doi.org/10.15252/embj.2019102236 Cite
Scientists highlight the development of in vitro cell culture models that allow systematic studies of pancreatic cell mechanobiology in response to extracellular matrix proteins, biomechanical effects, soluble factor modulation of biomechanics, substrate stiffness, fluid flow and topography.
[Frontiers in Bioengineering and Biotechnology]
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