Researchers generated an immortalized erythroid cell line from peripheral blood stem cells of a HbE/β-thalassemia patient. Morphological analysis showed the cells are proerythroblasts with some early basophilic erythroblasts, with no change in morphology over time in culture.
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Trakarnsanga, K., Tipgomut, C., Metheetrairut, C., Wattanapanitch, M., Khuhapinant, A., Poldee, S., Kurita, R., Nakamura, Y., Srisawat, C., & Frayne, J. (2020). Generation of an immortalised erythroid cell line from haematopoietic stem cells of a haemoglobin E/β-thalassemia patient. Scientific Reports, 10(1), 16798. https://doi.org/10.1038/s41598-020-73991-4 Cite
Researchers created human induced pluripotent stem cells (hiPSCs) from different MSC sources to evaluate the capacity of MSC-derived iPSCs to differentiate into any cell type of the human body and to serve as an alternative source for iPSC generation.
[Journal of Materials Chemistry B]
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Ababneh, N. A., Al-Kurdi, B., Barham, R., Ali, D., Sharar, N., Abuarqoub, D., Jafar, H., Salah, B., & Awidi, A. (2020). Derivation of three human induced pluripotent stem cell lines (JUCTCi014-A, JUCTCi015-A, JUCTCi016-A) from mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue and Wharton’s jelly samples. Stem Cell Research, 49, 102000. https://doi.org/10.1016/j.scr.2020.102000 Cite
Researchers reprogrammed fibroblasts from affected individuals and controls into induced pluripotent stem cells and differentiated them into photoreceptor precursor cells or retinal organoids.
[American Journal of Human Genetics]
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Bruijn, S. E. de, Fiorentino, A., Ottaviani, D., Fanucchi, S., Melo, U. S., Corral-Serrano, J. C., Mulders, T., Georgiou, M., Rivolta, C., Pontikos, N., Arno, G., Roberts, L., Greenberg, J., Albert, S., Gilissen, C., Aben, M., Rebello, G., Mead, S., Raymond, F. L., … Hardcastle, A. J. (2020). Structural Variants Create New Topological-Associated Domains and Ectopic Retinal Enhancer-Gene Contact in Dominant Retinitis Pigmentosa. The American Journal of Human Genetics, 0(0). https://doi.org/10.1016/j.ajhg.2020.09.002 Cite
Researchers established a human liver organoid (HLO) based screening model for analyzing DILI pathology at organoid resolution. HLO, generated from ten-different pluripotent stem cell lines, contained polarized immature hepatocytes with bile canaliculi-like architecture, establishing the unidirectional bile acid transport pathway.
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Shinozawa, T., Kimura, M., Cai, Y., Saiki, N., Yoneyama, Y., Ouchi, R., Koike, H., Maezawa, M., Zhang, R.-R., Dunn, A., Ferguson, A., Togo, S., Lewis, K., Thompson, W., Asai, A., & Takebe, T. (2020). High-Fidelity Drug Induced Liver Injury Screen Using Human PSC-derived Organoids. Gastroenterology, 0(0). https://doi.org/10.1053/j.gastro.2020.10.002 Cite
Allele Biotechnology and Pharmaceuticals, Inc. has been using mRNA as its core technology for creating cGMP-grade iPSC lines for regenerative medicine and oncology for over a decade. The have recently shown that mRNAs encoding the SARS-CoV-2 antigen proteins could be packaged in iPSCs and delivered in extracellular vesicles, potentially together with the viral proteins these mRNAs encode—a dual mRNA and protein vaccine, all naturally packaged in cells.
[Allele Biotechnology and Pharmaceuticals, Inc. (Businesswire, Inc.)]
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Both induced pluripotent stem cells derived from a Marfan syndrome (MFS)-patient and the line with the corrected fibrillin-1 mutation were differentiated to cardiomyocytes. Several functional analyses were performed on this model to study MFS related cardiomyopathy.
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Scientists used 3D cortical spheroids derived from human iPSCs to replicate the neurodevelopmental window. Their results demonstrated that hyaluronan preferentially surrounds nascent excitatory synapses.
Scientists postulate that exit from pluripotency involves intermediates that retain pluripotency while simultaneously exhibiting lineage-bias.
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Stavish, D., Böiers, C., Price, C., Frith, T. J. R., Halliwell, J., Saldaña-Guerrero, I., Wray, J., Brown, J., Carr, J., James, C., Barbaric, I., Andrews, P. W., & Enver, T. (2020). Generation and trapping of a mesoderm biased state of human pluripotency. Nature Communications, 11(1), 4989. https://doi.org/10.1038/s41467-020-18727-8 Cite
The author reviews two decades of research aimed at overcoming difficulties in the clinical use of ESCs and iPSCs, including practical issues that limit their use, including their inherent properties of tumorigenicity, immunogenicity, and heterogeneity.
[Cell Stem Cell]
Investigators optimized and validated a new Single-Cell Western method to assess protein expression in human induced pluripotent stem cell-derived cardiomyocytes.
[Journal of Molecular and Cellular Cardiology]
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The authors focus on the latest approaches to study neuroscience related topics with organoids, such as the use of assembloids, CRISPR technology, patch-clamp and optogenetics techniques and discuss how modern 3-dimensional biomaterials, miniaturized bioreactors and microfluidic chips can help to overcome the disadvantages of their use.
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Organoids, Assembloids, and Novel Biotechnology: Steps Forward in Developmental and Disease-Related Neuroscience - Alexios A. Panoutsopoulos, 2020. (n.d.). Retrieved October 1, 2020, from https://journals.sagepub.com/doi/10.1177/1073858420960112 Cite