Scientists describe a protocol to generate cardiomyocytes, cardiac fibroblasts and cardiac endothelial cells, the three principal cell types in the heart, from human induced pluripotent stem cells and combine them in 3D cardiac microtissues.
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Campostrini, G., Meraviglia, V., Giacomelli, E., van Helden, R. W. J., Yiangou, L., Davis, R. P., Bellin, M., Orlova, V. V., & Mummery, C. L. (2021). Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells. Nature Protocols, 1–44. https://doi.org/10.1038/s41596-021-00497-2 Cite
Using patient-derived induced pluripotent stem cells and CRISPR/Cas9 engineering, investigators developed a human model of Leigh syndrom caused by mutations in the complex IV assembly gene SURF1.
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Inak, G., Rybak-Wolf, A., Lisowski, P., Pentimalli, T. M., Jüttner, R., Glažar, P., Uppal, K., Bottani, E., Brunetti, D., Secker, C., Zink, A., Meierhofer, D., Henke, M.-T., Dey, M., Ciptasari, U., Mlody, B., Hahn, T., Berruezo-Llacuna, M., Karaiskos, N., … Prigione, A. (2021). Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndrome. Nature Communications, 12(1), 1929. https://doi.org/10.1038/s41467-021-22117-z Cite
Investigators generated human serotonergic neurons in vitro and detected secreted serotonin using aptamer-coated nanopipettes in a low nanomolar range, with the possibility of detecting significantly lower concentrations.
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Researchers compared two previously published protocols to obtain human induced pluripotent stem cell-derived striatal neurons from both healthy donors and Huntington’s disease patients.
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Le Cann, K., Foerster, A., Rösseler, C., Erickson, A., Hautvast, P., Giesselmann, S., Pensold, D., Kurth, I., Rothermel, M., Mattis, V. B., Zimmer-Bensch, G., von Hörsten, S., Denecke, B., Clarner, T., Meents, J., & Lampert, A. (2021). The difficulty to model Huntington’s disease in vitro using striatal medium spiny neurons differentiated from human induced pluripotent stem cells. Scientific Reports, 11(1), 6934. https://doi.org/10.1038/s41598-021-85656-x Cite
Scientists described a high yield approach for isolation and characterization of hESC-derived heart field specific and nodal-like cardiomyocytes.
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Isolation and characterization of hESC-derived heart field-specific cardiomyocytes unravels new insights into their transcriptional and electrophysiological profiles | Cardiovascular Research | Oxford Academic. (n.d.). Retrieved March 24, 2021, from https://academic.oup.com/cardiovascres/advance-article-abstract/doi/10.1093/cvr/cvab102/6179328?redirectedFrom=fulltext Cite
Scientists provide a simple method for the storage of neural stem cells as neurospheres at ambient temperature as an alternative method to more costly and inconvenient traditional methods of cryopreservation.
[Stem Cell Research & Therapy]
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Li, S., Zhao, H., Han, X., Ni, B., He, L., Mukama, O., de Dieu Habimana, J., Lin, Z., Huang, R., Huang, H., Tian, C., Tang, F., & Li, Z. (2021). Generation of UCiPSC-derived neurospheres for cell therapy and its application. Stem Cell Research & Therapy, 12(1), 188. https://doi.org/10.1186/s13287-021-02238-4 Cite
To selectively label dopaminergic neurons and analyze mitochondrial morphology at the ultrastructural level, investigators generated control and PRKN-mutated patient tyrosine hydroxylase reporter iPSC lines.
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Establishment of an in vitro model for analyzing mitochondrial ultrastructure in PRKN -mutated patient iPSC-derived dopaminergic neurons | Molecular Brain | Full Text. (n.d.). Retrieved March 24, 2021, from https://molecularbrain.biomedcentral.com/articles/10.1186/s13041-021-00771-0# Cite
The authors established a novel and robust monolayer differentiation method to produce functional neural progenitor cells (NPC) from hESC/iPSCs on Type I Collagen. The derived cells not only displayed the requisite markers, but also behaved similarly to classic NPCs both in vitro and in vivo.
[Science China LIfe Sciences]
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Liu, P., Chen, S., Wang, Y., Chen, X., Guo, Y., Liu, C., Wang, H., Zhao, Y., Wu, D., Shan, Y., Zhang, J., Wu, C., Li, D., Zhang, Y., Zhou, T., Chen, Y., Liu, X., Li, C., Wang, L., … Pei, D. (2021). Efficient induction of neural progenitor cells from human ESC/iPSCs on Type I Collagen. Science China Life Sciences. https://doi.org/10.1007/s11427-020-1897-0 Cite
To clarify the function of PARP1 in hESCs, scientists report a PARP1 knockout hESC line, generated by CRISPR/Cas9 mediated gene targeting. This cell line showed normal karyotype, pluripotent stem cell marker expression and differentiation potential in vitro.
[Stem Cell Research]
hiPSCs were derived from a long QT syndrome type 2 (LQT2) patient carrying the Per-Arnt-Sim (PAS) domain variant hERG1-H70R. CRISPR/Cas9 gene editing produced isogenic control iPSC lines. Differentiated iPSC-derived cardiomyocytes were evaluated for their electrophysiology, hERG1a/1b mRNA expression, and hERG1a/1b protein expression.
[Circulation-Arrhythmia and Electrophysiology]
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Feng Li, Zhang Jianhua, Lee ChangHwan, Kim Gina, Liu Fang, Petersen Andrew J., Lim Evi, Anderson Corey L., Orland Kate M., Robertson Gail A., Eckhardt Lee L., January Craig T., & Kamp Timothy J. (n.d.). Long QT Syndrome 2 PAS Domain Variant Induces hERG1a/1b Subunit Imbalance in Patient-specific iPSC-cardiomyocytes. Circulation: Arrhythmia and Electrophysiology, 0(0). https://doi.org/10.1161/CIRCEP.120.009343 Cite
Exacis Biotherapeutics Inc., announced several important steps in the preclinical development of its ExaNK™ engineered NK cell-therapy candidates.
[Exacis Biotherapeutics Inc. (PR Newswire LLC.)]
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