Tag Archives: induced pluripotent stem cells

Generation of an Immortalized Erythroid Cell Line from Hematopoietic Stem Cells of a Hemoglobin E/β-Thalassemia Patient

Researchers generated an immortalized erythroid cell line from peripheral blood stem cells of a HbE/β-thalassemia patient. Morphological analysis showed the cells are proerythroblasts with some early basophilic erythroblasts, with no change in morphology over time in culture.
[Scientific Reports]
Trakarnsanga, K., Tipgomut, C., Metheetrairut, C., Wattanapanitch, M., Khuhapinant, A., Poldee, S., Kurita, R., Nakamura, Y., Srisawat, C., & Frayne, J. (2020). Generation of an immortalised erythroid cell line from haematopoietic stem cells of a haemoglobin E/β-thalassemia patient. Scientific Reports, 10(1), 16798. https://doi.org/10.1038/s41598-020-73991-4 Cite
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Derivation of Three Human Induced Pluripotent Stem Cell Lines (JUCTCi014-A, JUCTCi015-A, JUCTCi016-a) from Mesenchymal Stem Cells (MSCs) Derived from Bone Marrow, Adipose Tissue and Wharton’s Jelly Samples

Researchers created human induced pluripotent stem cells (hiPSCs) from different MSC sources to evaluate the capacity of MSC-derived iPSCs to differentiate into any cell type of the human body and to serve as an alternative source for iPSC generation.
[Journal of Materials Chemistry B]
Ababneh, N. A., Al-Kurdi, B., Barham, R., Ali, D., Sharar, N., Abuarqoub, D., Jafar, H., Salah, B., & Awidi, A. (2020). Derivation of three human induced pluripotent stem cell lines (JUCTCi014-A, JUCTCi015-A, JUCTCi016-A) from mesenchymal stem cells (MSCs) derived from bone marrow, adipose tissue and Wharton’s jelly samples. Stem Cell Research, 49, 102000. https://doi.org/10.1016/j.scr.2020.102000 Cite
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Structural Variants Create New Topological-Associated Domains and Ectopic Retinal Enhancer-Gene Contact in Dominant Retinitis Pigmentosa

Researchers reprogrammed fibroblasts from affected individuals and controls into induced pluripotent stem cells and differentiated them into photoreceptor precursor cells or retinal organoids.
[American Journal of Human Genetics]
Bruijn, S. E. de, Fiorentino, A., Ottaviani, D., Fanucchi, S., Melo, U. S., Corral-Serrano, J. C., Mulders, T., Georgiou, M., Rivolta, C., Pontikos, N., Arno, G., Roberts, L., Greenberg, J., Albert, S., Gilissen, C., Aben, M., Rebello, G., Mead, S., Raymond, F. L., … Hardcastle, A. J. (2020). Structural Variants Create New Topological-Associated Domains and Ectopic Retinal Enhancer-Gene Contact in Dominant Retinitis Pigmentosa. The American Journal of Human Genetics, 0(0). https://doi.org/10.1016/j.ajhg.2020.09.002 Cite
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High-Fidelity Drug Induced Liver Injury Screen Using Human PSC-derived Organoids

Researchers established a human liver organoid (HLO) based screening model for analyzing DILI pathology at organoid resolution. HLO, generated from ten-different pluripotent stem cell lines, contained polarized immature hepatocytes with bile canaliculi-like architecture, establishing the unidirectional bile acid transport pathway.
[Gastroenterology]
Shinozawa, T., Kimura, M., Cai, Y., Saiki, N., Yoneyama, Y., Ouchi, R., Koike, H., Maezawa, M., Zhang, R.-R., Dunn, A., Ferguson, A., Togo, S., Lewis, K., Thompson, W., Asai, A., & Takebe, T. (2020). High-Fidelity Drug Induced Liver Injury Screen Using Human PSC-derived Organoids. Gastroenterology, 0(0). https://doi.org/10.1053/j.gastro.2020.10.002 Cite
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Allele Biotech’s cGMP iPSCs Used as Packaging Factories for Dual COVID Vaccine

Allele Biotechnology and Pharmaceuticals, Inc. has been using mRNA as its core technology for creating cGMP-grade iPSC lines for regenerative medicine and oncology for over a decade. The have recently shown that mRNAs encoding the SARS-CoV-2 antigen proteins could be packaged in iPSCs and delivered in extracellular vesicles, potentially together with the viral proteins these mRNAs encode—a dual mRNA and protein vaccine, all naturally packaged in cells.
[Allele Biotechnology and Pharmaceuticals, Inc. (Businesswire, Inc.)]
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Effects of Fibrillin Mutations on the Behavior of Heart Muscle Cells in Marfan Syndrome

Both induced pluripotent stem cells derived from a Marfan syndrome (MFS)-patient and the line with the corrected fibrillin-1 mutation were differentiated to cardiomyocytes. Several functional analyses were performed on this model to study MFS related cardiomyopathy.
[Scientific Reports]
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Hyaluronan Regulates Synapse Formation and Function in Developing Neural Networks

Scientists used 3D cortical spheroids derived from human iPSCs to replicate the neurodevelopmental window. Their results demonstrated that hyaluronan preferentially surrounds nascent excitatory synapses.
[Scientific Reports]
Wilson, E., Knudson, W., & Newell-Litwa, K. (2020). Hyaluronan regulates synapse formation and function in developing neural networks. Scientific Reports, 10(1), 16459. https://doi.org/10.1038/s41598-020-73177-y Cite
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Generation and Trapping of a Mesoderm Biased State of Human Pluripotency

Scientists postulate that exit from pluripotency involves intermediates that retain pluripotency while simultaneously exhibiting lineage-bias.
[Nature Communications]
Stavish, D., Böiers, C., Price, C., Frith, T. J. R., Halliwell, J., Saldaña-Guerrero, I., Wray, J., Brown, J., Carr, J., James, C., Barbaric, I., Andrews, P. W., & Enver, T. (2020). Generation and trapping of a mesoderm biased state of human pluripotency. Nature Communications, 11(1), 4989. https://doi.org/10.1038/s41467-020-18727-8 Cite
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Pluripotent Stem Cell-Based Cell Therapy—Promise and Challenges

The author reviews two decades of research aimed at overcoming difficulties in the clinical use of ESCs and iPSCs, including practical issues that limit their use, including their inherent properties of tumorigenicity, immunogenicity, and heterogeneity.
[Cell Stem Cell]
Yamanaka, S. (2020). Pluripotent Stem Cell-Based Cell Therapy—Promise and Challenges. Cell Stem Cell, 27(4), 523–531. https://doi.org/10.1016/j.stem.2020.09.014 Cite
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Single-Cell Protein Expression of hiPSC-Derived Cardiomyocytes Using Single-Cell Westerns

Investigators optimized and validated a new Single-Cell Western method to assess protein expression in human induced pluripotent stem cell-derived cardiomyocytes.
[Journal of Molecular and Cellular Cardiology]
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Organoids, Assembloids, and Novel Biotechnology: Steps Forward in Developmental and Disease-Related Neuroscience

The authors focus on the latest approaches to study neuroscience related topics with organoids, such as the use of assembloids, CRISPR technology, patch-clamp and optogenetics techniques and discuss how modern 3-dimensional biomaterials, miniaturized bioreactors and microfluidic chips can help to overcome the disadvantages of their use.
[Neuroscientist]
Organoids, Assembloids, and Novel Biotechnology: Steps Forward in Developmental and Disease-Related Neuroscience - Alexios A. Panoutsopoulos, 2020. (n.d.). Retrieved October 1, 2020, from https://journals.sagepub.com/doi/10.1177/1073858420960112 Cite
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