Atypical Cyclin P Regulates Cancer Cell Stemness through Activation of the WNT Pathway

Cell line-derived spheroids, ex vivo intestinal organoid cultures and induced-pluripotent stem cells were used to investigate the role of atypical cyclin P in stemness.
[Cellular Oncology]
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PLCγ2 Regulates TREM2 Signaling and Integrin-Mediated Adhesion and Migration of Human iPSC-Derived Macrophages

To explore the relationship between PLCγ2 and TREM2 and the role of PLCγ2 in regulating immune cell function, the authors generated human iPSC- derived macrophages from isogenic lines with homozygous PLCG2 knockout.
[Scientific Reports]
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Loss of Resf1 Reduces the Efficiency of Embryonic Stem Cell Self-Renewal and Germline Entry

The authors used Resf1 knockout cell lines to determine the requirements of RESF1 for ESC self-renewal and for in vitro specification of ESCs into primordial germ cell-like cells
[Life Science Alliance]
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Stem Cell Therapy in Liver Regeneration: Focus on Mesenchymal Stem Cells and Induced Pluripotent Stem Cells

Owing to the recent developments in mesenchymal stem cells and induced pluripotent stem cells, combined with emerging technologies including graphene, nano-biomaterials, and gene editing, precision medicine and individualized clinical treatment may be realized in the near future.
[Pharmacology & Therapeutics]
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Safe and Stable Generation of Induced Pluripotent Stem Cells Using Doggybone DNA Vectors

Researchers reported the first ever application of doggybone DNA (dbDNA™) vectors to generate human iPSCs. They achieved comparable iPSC reprogramming efficiencies using transiently expressing dbDNA™ vectors with the same iPSC reprogramming coding sequences as the state-of-the-art OriP/EBNA1 episomal vectors, in the absence of p53 shRNA repression.
[Molecular Therapy-Methods & Clinical Development]
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Large-Scale Analysis of Imprinting in Naive Human Pluripotent Stem Cells Reveals Recurrent Aberrations and a Potential Link to FGF Signaling

Scientists analyzed loss of imprinting (LOI) in a large cohort of both bulk and single-cell RNA sequencing samples of naive and primed human PSCs (hPSCs). They showed that naive hPSCs exhibited high levels of non-random LOI, with bias toward paternally methylated imprinting control regions.
[Stem Cell Reports]
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Metabolic and Epigenetic Regulation of Endoderm Differentiation

Human PSCs hold great promise for generating endodermal cells and their derivatives for the development of new therapeutics against many life-threatening diseases. Scientists review recent advances in metabolic and epigenetic regulation of endoderm differentiation.
[Trends in Cell Biology]
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Modeling Congenital Cataract In Vitro Using Patient-Specific Induced Pluripotent Stem Cells

Scientists reported the establishment of a human congenital cataract in vitro model through differentiation of patient-specific iPSCs into regenerated lenses.
[NPJ Regenerative Medicine]
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Mammalian In Vitro Gametogenesis

The past decade has seen considerable advances in in vitro gametogenesis, which aims to recreate germ cell development from PSCs in culture. Mouse PSCs can be induced into functional oocytes and spermatozoa, whereas human PSCs can be induced into early oocytes and prospermatogonia, promoting mechanistic understanding of mammalian germ cell development.
[Science]
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Master Regulators of Skeletal Muscle Lineage Development and Pluripotent Stem Cells Differentiation

The expression of genes MYF5, MRF4, MYOD is activated by muscle progenitors throughout development, in several waves of myogenic differentiation, constituting the embryonic, fetal and postnatal phases of muscle growth.
[Cell Regeneration]
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Droplet-Based Vitrification of Adherent Human Induced Pluripotent Stem Cells on Alginate Microcarrier Influenced by Adhesion Time and Matrix Elasticity

Investigators presented a novel vitrification-based method for adherent human iPSCs, designed for automated handling by microfluidic approaches and with ready-to-use potential, in suspension-based bioreactors after thawing, for example.
[Cryobiology]
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