induced pluripotent stem cells

[Stem Cell Reports] Investigators presented a bottom-up engineering approach to building vascularized human tissue by combining genetic reprogramming with chemically directed organoid differentiation.

[STAR Protocols] Researchers developed erythroblasts with custom phenotypes using CRISPR/Cas9 gene-editing of a human iPSC parent line for the blood group system genes: RHAG, GYPB and XK.

[Molecular and Cellular Biochemistry] The authors investigated the contribution of the Bax, Sirt-1, TGF-β, and MALAT genes and/or their protein expression to the pathogenesis of submandibular carcinogenesis before and after iPSCs treatment.

[STAR Protocols] Investigators provided a detailed protocol for the transfection of mouse ESCs and mouse-iPSCs with a Zscan5b constitutive expression plasmid, using the piggyBac transposon gene expression vector system.

[Scientific Reports] Researchers presented human patient induced pluripotent stem cell-derived neurons cultured on densely-spaced spiky silicon nanowire arrays which showed mature electrophysiological characteristics after only 20 days of culturing.

[Nature Communications] Researchers attempted to model human early embryo development with expanded PSCs (EPSCs) in 3-dimensions. They defined a protocol that allowed them to generate self-organizing cystic structures from human EPSCs that displayed some hallmarks of human early embryogenesis.