Synergistic Gene Editing in Human iPS Cells via Cell Cycle and DNA Repair Modulation

Scientists established a fluorescent DNA repair assay in human iPSCs to visualize and quantify the frequency of DNA repair outcomes during monoallelic and biallelic targeting.
[Nature Communications]
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N-3 Polyunsaturated Fatty Acids Promote Astrocyte Differentiation and Neurotrophin Production Independent of cAMP in Patient-Derived Neural Stem Cells

iPSC-derived neuronal stem cell lines were generated from individuals with major depressive disorder. Astrocytes differentiated from patient-derived neuronal stem cells were verified by GFAP and cells were treated with eicosapentaenoic acid, docosahexaenoic acid or stearic acid.
[Molecular Psychiatry]
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Acute Myeloid Leukemia iPSCs Reveal a Role for RUNX1 in the Maintenance of Human Leukemia Stem Cells

Researchers report that genetically clonal iPSCs derived from an acute myeloid leukemia patient and characterized by exceptionally high engraftment potential gave rise, upon hematopoietic differentiation, to a phenotypic hierarchy.
[Cell Reports]
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Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells

Improving methods for liver decellularization, recellularization, and differentiation of different liver cellular lineages of human iPSCs in an organ-like environment, investigators generated functional engineered human mini-livers and performed transplantation in a rat model.
[Cell Reports]
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Prevention of Tumor Risk Associated with the Reprogramming of Human Pluripotent Stem Cells

The authors focus on the risk of tumor formation by human PSCs, and on the possible treatment options if it occurs. Potential new techniques that target epigenetic processes and chromatin regulation provide opportunities for human cancer modeling and regenerative medicine.
[Journal of Experimental & Clinical Cancer Research]
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International Prize Awarded to Two Pieces of Novel Research in the 3Rs

The 2019 3Rs prize has been jointly awarded to Dr Francesca Nunn and Dr Marta Shahbazi. Marta and colleagues have developed advanced 3D cultures of human and mouse ESCs to mimic the development of the embryo at implantation and the subsequent morphogenesis and formation of the amniotic cavity.
[EurekAlert!]
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Improving the Safety of Human Pluripotent Stem Cell Therapies Using Genome-Edited Orthogonal Safeguards

Scientists used genome editing to engineer a general platform to improve the safety of future human pluripotent stem cell-derived cell transplantation therapies.
[Nature Communications]
Martin, R. M., Fowler, J. L., Cromer, M. K., Lesch, B. J., Ponce, E., Uchida, N., Nishimura, T., Porteus, M. H., & Loh, K. M. (2020). Improving the safety of human pluripotent stem cell therapies using genome-edited orthogonal safeguards. Nature Communications, 11(1), 2713. https://doi.org/10.1038/s41467-020-16455-7 Cite
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Human-iPSC-Derived Cardiac Stromal Cells Enhance Maturation in 3D Cardiac Microtissues and Reveal Non-cardiomyocyte Contributions to Heart Disease

Scientists showed that tri-cellular combinations of human induced pluripotent stem cells (iPSC)-derived cardiomyocytes, cardiac fibroblasts, and cardiac endothelial cells enhanced maturation in easily constructed, scaffold-free, three-dimensional microtissues.
[Cell Stem Cell]
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Endothelial-to-Mesenchymal Transition in Anticancer Therapy and Normal Tissue Damage

The authors review the role of hypoxia and reactive oxygen species as the main stimulating factors of tissue damage due to vascular damage and endothelial-to-mesenchymal transition (EndMT). They consider drugs that may be clinically useful for regulating EndMT in various diseases.
[Experimental and Molecular Medicine]
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