induced pluripotent stem cells

[Islets] Scientists review the attempts over the past decade, to utilize the organoid cell culture principles in order to identify, expand, and differentiate the adult pancreatic stem cells from different compartments of mouse and human pancreata.

[Cell Stem Cell] Xenotransplantation of human microglia into cerebral organoids allowed us to characterize key transcriptional programs of developing microglia in vitro and revealed that microglia induced transcriptional changes in neural stem cells and decrease interferon signaling response genes.

[iScience] Brain organoids successfully mimicked patient phenotypes not reliably reproduced by existing models, including decreased alpha dystroglycan glycosylation and abnormal radial glial cell fiber migration.

[Scientific Reports] Kruppel-like factor 15 (KLF15) was identified as a new regulator of hepatic maturation through a comprehensive analysis of the expression of transcriptional regulators in mouse fetal and adult hepatocytes. KLF15 induced the overexpression of liver function genes in mouse embryonic hepatocytes.

[Translational Psychiatry] Spinocerebellar ataxia type 3 (SCA3)-iPSCs could be corrected by the replacement of the abnormal CAG expansions with normal repeats using CRISPR/Cas9-mediated homologous recombination strategy. Corrected SCA3-iPSCs retained pluripotent and normal karyotype, which could be differentiated into a neural stem cell and neuronal cells.

[Molecular Psychiatry] Investigators used iPSC technology to obtain hippocampal neurons to investigate the neuropathology of IQSEC2-mediated disease. The neurons were characterized at three-time points during differentiation to assess developmental progression.