Tag Archives: keratinocytes

Integrative Transcriptomic Analysis for Linking Acute Stress Responses to Squamous Cell Carcinoma Development

In vitro, Oncostatin M promoted invasiveness of keratinocytes and cutaneous squamous cell carcinoma cells and suppressed apoptosis of irradiated keratinocytes.
[Scientific Reports]
Nguyen, T. N., Rajapakshe, K., Nicholas, C., Tordesillas, L., Ehli, E. A., Davis, C. M., Coarfa, C., Flores, E. R., Dickinson, S. E., Curiel-Lewandrowski, C., & Tsai, K. Y. (2020). Integrative transcriptomic analysis for linking acute stress responses to squamous cell carcinoma development. Scientific Reports, 10(1), 17209. https://doi.org/10.1038/s41598-020-74051-7 Cite
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Establishment and Validation of an In Vitro Co-Culture Model for Oral Cell Lines Using Human PBMC-Derived Osteoclasts, Osteoblasts, Fibroblasts and Keratinocytes

Researchers investigated the viability and proliferation of osteoblasts, fibroblasts and oral keratinocytes under stratified medium modification and assessed the differentiation of osteoclasts in each co-culture.
[Scientific Reports]
Establishment and validation of an in vitro co-culture model for oral cell lines using human PBMC-derived osteoclasts, osteoblasts, fibroblasts and keratinocytes | Scientific Reports. (n.d.). Retrieved October 8, 2020, from https://www.nature.com/articles/s41598-020-73941-0 Cite
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EZH2-Dependent Epigenetic Modulation of Histone H3 Lysine-27 Contributes to Psoriasis by Promoting Keratinocyte Proliferation

The authors found that EZH2 and H3K27me3 were both overexpressed in the epidermis of psoriatic lesional skin compared to normal skin. In vitro, the expression of EZH2 and H3K27me3 was stimulated in human keratinocytes treated with mixture of psoriasis-related cytokines pool.
[Cell Death & Disease]
EZH2-dependent epigenetic modulation of histone H3 lysine-27 contributes to psoriasis by promoting keratinocyte proliferation | Cell Death & Disease. (n.d.). Retrieved October 5, 2020, from https://www.nature.com/articles/s41419-020-03028-1 Cite
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Staphylococcus aureus Second Immunoglobulin-Binding Protein Drives Atopic Dermatitis via IL-33

In vitro human keratinocyte cell culture, ex vivo human skin organ explants and the eczema prone Nc/Tnd mouse were used as model systems to assess type-2 promoting immune responses to S. aureus.
[Journal of Allergy and Clinical Immunology]
Kindi, A. A., Williams, H., Matsuda, K., Alkahtani, A. M., Saville, C., Bennett, H., Alshammari, Y., Tan, S. Y., O’Neill, C., Tanaka, A., Matsuda, H., Arkwright, P. D., & Pennock, J. L. (2020). Staphylococcus aureus Second Immunoglobulin-Binding Protein drives atopic dermatitis via IL-33. Journal of Allergy and Clinical Immunology, 0(0). https://doi.org/10.1016/j.jaci.2020.09.023 Cite
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Markers for Ca++-Induced Terminal Differentiation of Keratinocytes In Vitro Under Defined Conditions

Researchers demonstrated that among 18 markers of terminally differentiated keratinocytes of stratum granulosum and stratum corneum in vivo, only four (CDSN, KPRP, LCE1C, and SPRR4) have reproduced their expression pattern in vitro.
[Experimental Dermatology]
Jeriha, J., Kolundzic, N., Khurana, P., Gordon, M., Celli, A., Mauro, T. M., & Ilic, D. (n.d.). Markers for Ca++-induced terminal differentiation of keratinocytes in vitro under defined conditions. Experimental Dermatology, n/a(n/a). https://doi.org/10.1111/exd.14199 Cite
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Exploitation of Filamentous and Picoplanktonic Cyanobacteria for Cosmetic Applications: Potential to Improve Skin Structure and Preserve Dermal Matrix Components

Filamentous and picoplanktonic cyanobacteria extracts were analyzed for their pigment profile, phenolic content, antioxidant potential, cytotoxicity against keratinocytes, fibroblasts, endothelial cells and capacity to inhibit hyaluronidase.
[Marine Drugs]
Morone, J., Lopes, G., Preto, M., Vasconcelos, V., & Martins, R. (2020). Exploitation of Filamentous and Picoplanktonic Cyanobacteria for Cosmetic Applications: Potential to Improve Skin Structure and Preserve Dermal Matrix Components. Marine Drugs, 18(9), 486. https://doi.org/10.3390/md18090486 Cite
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Type XVII Collagen Interacts with the aPKC‐PAR Complex and Maintains Epidermal Cell Polarity

Investigators uncovered COL17 as a binding partner of the aPKC‐PAR complex, which is a key regulating factor of cell polarity. Immunoprecipitation‐immunoblot assay and protein‐protein binding assay revealed that COL17 interacts with aPKC and PAR3. Type XVII collagen (COL17) deficiency or epidermis‐specific aPKCλ deletion destabilized PAR3 distribution in the epidermis, while aPKCζ knockout did not.
[Experimental Dermatology]
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Optimization of Human Papillomavirus-Based Pseudovirus Techniques for Efficient Gene Transfer

Scientists describe optimization of current methods and present new protocols for using human papillomavirus capsids to deliver non-viral DNA, thereby providing an alternative to DNA transfection. Using keratinocyte generated extracellular matrices could enhance infection efficiency in keratinocytes, hepatocytes and neuronal cells.
[Scientific Reports]
Gilson, T. D., Gibson, R. T., & Androphy, E. J. (2020). Optimization of human papillomavirus-based pseudovirus techniques for efficient gene transfer. Scientific Reports, 10(1), 15517. https://doi.org/10.1038/s41598-020-72027-1 Cite
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Early-Stage Bilayer Tissue-Engineered Skin Substitute Formed by Adult Skin Progenitor Cells Produces an Improved Skin Structure In Vivo

Adult scalp dermal progenitor cells and epidermal stem cells together with type I collagen as a scaffold material were used to reconstitute bilayer tissue-engineered skin substitutes in vitro.
[Stem Cell Research & Therapy]
Zhang, Q., Wen, J., Liu, C., Ma, C., Bai, F., Leng, X., Chen, Z., Xie, Z., Mi, J., & Wu, X. (2020). Early-stage bilayer tissue-engineered skin substitute formed by adult skin progenitor cells produces an improved skin structure in vivo. Stem Cell Research & Therapy, 11(1), 407. https://doi.org/10.1186/s13287-020-01924-z Cite
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Keratinocyte Autophagy Enables the Activation of Keratinocytes and Fibroblasts and Facilitates Wound Healing

Using cytokine array screening, scientists found that autophagy deficiency inhibited the transcription and production of the cytokine CCL2/MCP-1 by TNF.
[Autophagy]
Qiang, L., Yang, S., Cui, Y.-H., & He, Y.-Y. (2020). Keratinocyte autophagy enables the activation of keratinocytes and fibroblasts and facilitates wound healing. Autophagy, 0(0), 1–16. https://doi.org/10.1080/15548627.2020.1816342 Cite
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New Insight Into the Role of Exosomes in Vitiligo

The authors investigate the association of exosomes with vitiligo and emphasize the role of exosomes in immune regulation, melanocyte–keratinocyte interactions, and melanogenesis.
[Autoimmunity Reviews]
Wong, P. M., Yang, L., Yang, L., Wu, H., Li, W., Ma, X., Katayama, I., & Zhang, H. (2020). New insight into the role of exosomes in vitiligo. Autoimmunity Reviews, 102664. https://doi.org/10.1016/j.autrev.2020.102664 Cite
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