lentiviral transduction

[Nature Protocols] Researchers provided a detailed technical pipeline and protocol for using the CRISPR–Cas9 genome editing system to knock out a gene of interest specifically in Human intestinal tissue–derived enteroids by lentiviral transduction and single-cell cloning.

[BMC Biology] Researchers investigated the potential of bacteriophage-chimeric retrovirus-like particles for the non-integrative delivery of RNA molecules in human iPSCs for CRISPR/Cas9 applications.

[BMC Cancer] Researchers hypothesized that IL8 might regulate migration, invasion, and cisplatin resistance of CD10-positive oral cancer cells through the ERK pathway.

[Journal of Experimental & Clinical Cancer Research] Researchers indicated that the modulation of NF-YA isoforms affected prostate pathophysiological processes and contributed to a cancer-relevant phenotype, in vitro and in vivo.

[Frontiers in Immunology] Scientists described an anti-HLA-A2 CAR generated by grafting the complementarity-determining regions of a human monoclonal anti-HLA-A2 antibody into the framework regions of the Herceptin 4D5 single-chain variable fragment and fusing it with a CD28-ζ signaling domain.

[NPJ Regenerative Medicine] Using SPRY4 gene delivery into healthy and degenerated chondrocytes, investigators elucidated the role of SPRY4 in preventing chondrocyte hypertrophy. In addition to using the human cartilage tissues with the destabilization of the model in Sprague-Dawley rats, the role of SPRY4 in cartilage tissues and chondrocytes was explored through their molecular and histological analysis.