Genome-Wide CRISPR/Cas9-Knockout in Human Induced Pluripotent Stem Cell (iPSC)-Derived Macrophages

Scientists present an efficient, lentiviral transduction protocol for delivery of CRISPR/Cas9 to macrophages derived from human iPSC with efficiencies close to 100%.
[Scientific Reports]
Navarro-Guerrero, E., Tay, C., Whalley, J. P., Cowley, S. A., Davies, B., Knight, J. C., & Ebner, D. (2021). Genome-wide CRISPR/Cas9-knockout in human induced Pluripotent Stem Cell (iPSC)-derived macrophages. Scientific Reports, 11(1), 4245. https://doi.org/10.1038/s41598-021-82137-z Cite
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Alpha-Enolase (ENO1), Identified as an Antigen to Monoclonal Antibody 12C7, Promotes the Self-Renewal and Malignant Phenotype of Lung Cancer Stem Cells by AMPK/mTOR Pathway

Immunofluorescence was used for antigen localization. After targeted antigen purification by electrophoresis and immunoblot, the antigen was identified by LC-MALDI-TOF/TOF mass spectrometry, immunofluorescence, and immunoprecipitation.
[Stem Cell Research & Therapy]
Shu, X., Cao, K.-Y., Liu, H.-Q., Yu, L., Sun, L.-X., Yang, Z.-H., Wu, C.-A., & Ran, Y.-L. (2021). Alpha-enolase (ENO1), identified as an antigen to monoclonal antibody 12C7, promotes the self-renewal and malignant phenotype of lung cancer stem cells by AMPK/mTOR pathway. Stem Cell Research & Therapy, 12(1), 119. https://doi.org/10.1186/s13287-021-02160-9 Cite
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CD10 Marks Non-Canonical PPARγ-Independent Adipocyte Maturation and Browning Potential of Adipose-Derived Stem Cells

Investigators identified intrinsic CD10 levels as a positive determinant of adipocyte maturation as well as browning potential of human adipose-derived stem cells.
[Stem Cell Research & Therapy]
Chakraborty, S., Ong, W. K., Yau, W. W. Y., Zhou, Z., Bhanu Prakash, K. N., Toh, S.-A., Han, W., Yen, P. M., & Sugii, S. (2021). CD10 marks non-canonical PPARγ-independent adipocyte maturation and browning potential of adipose-derived stem cells. Stem Cell Research & Therapy, 12(1), 109. https://doi.org/10.1186/s13287-021-02179-y Cite
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Hematopoietic-Stem-Cell-Targeted Gene-Addition and Gene-Editing Strategies for β-hemoglobinopathies

State-of-the-art gene-editing technology has made it possible to repair the β-globin mutation in patient hematopoietic stem cells or target genetic loci associated with reactivation of endogenous γ-globin expression.
[Cell Stem Cell]
Drysdale, C. M., Nassehi, T., Gamer, J., Yapundich, M., Tisdale, J. F., & Uchida, N. (2021). Hematopoietic-Stem-Cell-Targeted Gene-Addition and Gene-Editing Strategies for β-hemoglobinopathies. Cell Stem Cell, 28(2), 191–208. https://doi.org/10.1016/j.stem.2021.01.001 Cite
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Combined Lentiviral- and RNA-Mediated CRISPR/Cas9 Delivery for Efficient and Traceable Gene Editing in Human Hematopoietic Stem and Progenitor Cells

Researchers developed a combinatorial system using stable lentiviral delivery of single guide RNA followed by transient transfection of Cas9 mRNA by electroporation in human cord blood-derived CD34+ hematopoietic stem and progenitor cells.
[Scientific Reports]
Yudovich, D., Bäckström, A., Schmiderer, L., Žemaitis, K., Subramaniam, A., & Larsson, J. (2020). Combined lentiviral- and RNA-mediated CRISPR/Cas9 delivery for efficient and traceable gene editing in human hematopoietic stem and progenitor cells. Scientific Reports, 10(1), 22393. https://doi.org/10.1038/s41598-020-79724-x Cite
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Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia

Researchers identified the cell types that contribute to CFTR expression and function within the proximal-distal axis of the normal human lung. Single cell RNA-seq was performed on freshly isolated human large and small airway epithelial cells.
[American Journal of Respiratory and Critical Care Medicine]
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