Alternative Splicing of NF-YA Promotes Prostate Cancer Aggressiveness and Represents a New Molecular Marker for Clinical Stratification of Patients

Researchers indicated that the modulation of NF-YA isoforms affected prostate pathophysiological processes and contributed to a cancer-relevant phenotype, in vitro and in vivo.
[Journal of Experimental & Clinical Cancer Research]
[zotpress userid=” items=’AAAAAAAA’ style=’apa’ cite=’yes’]
Full Article
Bookmark

No account yet? Register

Share

Precision Engineering of an Anti-HLA-A2 Chimeric Antigen Receptor in Regulatory T Cells for Transplant Immune Tolerance

Scientists described an anti-HLA-A2 CAR generated by grafting the complementarity-determining regions of a human monoclonal anti-HLA-A2 antibody into the framework regions of the Herceptin 4D5 single-chain variable fragment and fusing it with a CD28-ζ signaling domain.
[Frontiers in Immunology]
[zotpress userid=’6445218′ items=’AAAAAAAA’ style=’apa’ cite=’yes’]
Full Article
Bookmark

No account yet? Register

Share

SPRY4 Acts as an Indicator of Osteoarthritis Severity and Regulates Chondrocyte Hypertrophy and ECM Protease Expression

Using SPRY4 gene delivery into healthy and degenerated chondrocytes, investigators elucidated the role of SPRY4 in preventing chondrocyte hypertrophy. In addition to using the human cartilage tissues with the destabilization of the model in Sprague-Dawley rats, the role of SPRY4 in cartilage tissues and chondrocytes was explored through their molecular and histological analysis.
[NPJ Regenerative Medicine]
[zotpress userid=’7992332′ items=’AAAAAAAA’ style=’apa’ cite=’yes’]
Full Article
Bookmark

No account yet? Register

Share

Application of Human Liver Organoids as a Patient-Derived Primary Model for HBV Infection and Related Hepatocellular Carcinoma

Researchers described a primary ex vivo hepatitis B virus (HBV)-infection model derived from healthy donor liver organoids after challenge with recombinant virus or HBV-infected patient serum.
[eLife]
[zotpress userid=” items=’JRCUQR3X’ style=’apa’ cite=’yes’]
Abstract
Bookmark

No account yet? Register

Share

mRNA-Engineered Mesenchymal Stromal Cells Expressing CXCR2 Enhances Cell Migration and Improves Recovery in Inflammatory Bowel Disease

A new strategy to modify MSCs to express specific chemokine receptors using mRNA engineering was developed to evaluate the homing ability of MSCs and its therapeutic effects for inflammatory bowel disease.
[Molecular Therapy-Nucleic Acids]
[zotpress userid=” items=’2W7N6HI8′ style=’apa’ cite=’yes’]
AbstractFull ArticleGraphical Abstract
Bookmark

No account yet? Register

Share

Pulmonary Transplantation of Alpha-1 Antitrypsin (AAT)-Transgenic Macrophages Provides a Source of Functional Human AAT In Vivo

The authors investigated the pulmonary transplantation of macrophages transgenic for alpha-1 antitrypsin (AAT) as a potential therapy for AAT deficiency-associated lung pathology.
[Gene Therapy]
[zotpress userid=’6445218′ items=’SLXZ2G9Z’ style=’apa’ cite=’yes’]
Full Article
Bookmark

No account yet? Register

Share

Distinct Effects of V617F and exon12-Mutated JAK2 Expressions on Erythropoiesis in a Human Induced Pluripotent Stem Cell (iPSC)-Based Model

The iPSCs were used to model JAK2-mutated MPNs. Normal iPSCs underwent lentiviral transduction to overexpress JAK2p.V617F or JAK2p.N542_E543del under a doxycycline-inducible system. The modified iPSCs were differentiated into erythroid cells.
[Scientific Reports]
[zotpress userid=’6606345′ items=’V7HBTG2E’ style=’apa’ cite=’yes’]
Full Article
Bookmark

No account yet? Register

Share

Cutaneous and Hepatic Vascular Lesions Due to a Recurrent Somatic GJA4 Mutation Reveal a Pathway for Vascular Malformation

The authors found that the mutant induced changes in cell morphology and activated SGK1, a serine/threonine kinase known to regulate cell proliferation and apoptosis, via non-canonical activation.
[HGG Advances]
[zotpress userid=’6445212′ items=’3WNXVDIY’ style=’apa’ cite=’yes’]
AbstractFull Article
Bookmark

No account yet? Register

Share

Genome-Wide CRISPR/Cas9-Knockout in Human Induced Pluripotent Stem Cell (iPSC)-Derived Macrophages

Scientists present an efficient, lentiviral transduction protocol for delivery of CRISPR/Cas9 to macrophages derived from human iPSC with efficiencies close to 100%.
[Scientific Reports]
[zotpress userid=’6630899′ items=’DNU6APL3′ style=’apa’ cite=’yes’]
Full Article
Bookmark

No account yet? Register

Share

Alpha-Enolase (ENO1), Identified as an Antigen to Monoclonal Antibody 12C7, Promotes the Self-Renewal and Malignant Phenotype of Lung Cancer Stem Cells by AMPK/mTOR Pathway

Immunofluorescence was used for antigen localization. After targeted antigen purification by electrophoresis and immunoblot, the antigen was identified by LC-MALDI-TOF/TOF mass spectrometry, immunofluorescence, and immunoprecipitation.
[Stem Cell Research & Therapy]
[zotpress userid=’6445218′ items=’DQPEC8ZV’ style=’apa’ cite=’yes’]
Full Article
Bookmark

No account yet? Register

Share

CD10 Marks Non-Canonical PPARγ-Independent Adipocyte Maturation and Browning Potential of Adipose-Derived Stem Cells

Investigators identified intrinsic CD10 levels as a positive determinant of adipocyte maturation as well as browning potential of human adipose-derived stem cells.
[Stem Cell Research & Therapy]
[zotpress userid=’6630899′ items=’4VY6W5IB’ style=’apa’ cite=’yes’]
Full Article
Bookmark

No account yet? Register

Share
Share