Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies

Investigators developed two hiPSC/hESC-specific glycan-recognizing mouse antibodies, R-10G and R-17F, using the Tic hiPSC line as an antigen. R-10G recognized a low-sulfate keratan sulfate, and R-17F recognized lacto-N-fucopentaose-1.
[Biomolecules]
Nagai, Y., Nakao, H., Kojima, A., Komatsubara, Y., Ohta, Y., Kawasaki, N., Kawasaki, N., Toyoda, H., & Kawasaki, T. (2021). Glycan Epitopes on 201B7 Human-Induced Pluripotent Stem Cells Using R-10G and R-17F Marker Antibodies. Biomolecules, 11(4), 508. https://doi.org/10.3390/biom11040508 Cite
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Novel Candidate Factors Predicting the Effect of S-1 Adjuvant Chemotherapy of Pancreatic Cancer

Collection of an adequate number of PDAC cells is difficult due to the surrounding fibroblasts. The authors discovered novel biomarkers to predict chemosensitivity based on the collagen gel droplet-embedded drug sensitivity test results.
[Scientific Reports]
Mitachi, K., Ariake, K., Shima, H., Sato, S., Miura, T., Maeda, S., Ishida, M., Mizuma, M., Ohtsuka, H., Kamei, T., Igarashi, K., & Unno, M. (2021). Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer. Scientific Reports, 11(1), 6541. https://doi.org/10.1038/s41598-021-86099-0 Cite
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Lipid Profiling of Mouse Intestinal Organoids for Studying APC Mutations

Researchers investigated intra-cellular lipid disturbances induced by adenomatous polyposis coli (APC) gene mutations in intestinal organoids using a reversed-phase ultra-high-performance liquid chromatography mass spectrometry-based lipid profiling method.
[Bioscience Reports]
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Identification of Pannexin 1-Regulated Genes, Interactome, and Pathways in Rhabdomyosarcoma and Its Tumor Inhibitory Interaction with AHNAK

Scientists surveyed and characterized the molecular changes induced by PANX1 re-expression in rhabdomyosarcoma. They cataloged transcriptomic changes in this context by RNA sequencing.
[Oncogene]
Xiang, X., Langlois, S., St-Pierre, M.-E., Blinder, A., Charron, P., Graber, T. E., Fowler, S. L., Baird, S. D., Bennett, S. A. L., Alain, T., & Cowan, K. N. (2021). Identification of pannexin 1-regulated genes, interactome, and pathways in rhabdomyosarcoma and its tumor inhibitory interaction with AHNAK. Oncogene, 1–16. https://doi.org/10.1038/s41388-020-01623-2 Cite
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Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies

Scientists demonstrated that electromembrane extraction based on electrophoresis across an oil membrane was suited for segregating selected organoid-derived drug metabolites prior to mass spectrometry-based measurements.
[Analytical Chemistry]
Skottvoll, F. S., Hansen, F. A., Harrison, S., Boger, I. S., Mrsa, A., Restan, M. S., Stein, M., Lundanes, E., Pedersen-Bjergaard, S., Aizenshtadt, A., Krauss, S., Sullivan, G., Bogen, I. L., & Wilson, S. R. (2021). Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies. Analytical Chemistry. https://doi.org/10.1021/acs.analchem.0c05082 Cite
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NEDD9 is a Novel and Modifiable Mediator of Platelet-Endothelial Adhesion in the Pulmonary Circulation

Data from liquid chromatography-mass spectrometry and microscale thermophoresis informed the development of a novel antibody to inhibit platelet-endothelial adhesion, which was tested in cells from chronic thromboembolic pulmonary hypertension patients and three animal models in vivo.
[American Journal of Respiratory and Critical Care Medicine]
Alba, G. A., Samokhin, A. O., Wang, R.-S., Zhang, Y.-Y., Wertheim, B. M., Arons, E., Greenfield, E. A., Lundberg Slingsby, M. H., Ceglowski, J. R., Haley, K. J., Bowman, F. P., Yu, Y.-R., Haney, J. C., Eng, G., Mitchell, R. N., Sheets, A., Vargas, S. O., Seo, S., Channick, R. N., … Maron, B. A. (2021). NEDD9 is a Novel and Modifiable Mediator of Platelet-Endothelial Adhesion in the Pulmonary Circulation. American Journal of Respiratory and Critical Care Medicine. https://doi.org/10.1164/rccm.202003-0719OC Cite
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EEF1A2 Interacts with HSP90AB1 to Promote Lung Adenocarcinoma Metastasis via Enhancing TGF-β/SMAD Signaling

Scientists report that eukaryotic protein translation elongation factor 1α2 (EEF1A2) mediates the epithelial–mesenchymal transformation, to promote the metastasis of lung adenocarcinoma cells in vitro and in vivo.
[British Journal of Cancer]
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Isolation and Purification of Glycoglycerolipids To Induce Apoptosis in Breast Cancer Cells

Researchers investigated the therapeutic efficacy of cyanobacteria derived monogalactosyldiacylglycerol to inhibit breast cancer cell growth.
[Scientific Reports]
Abedin, M. R., & Barua, S. (2021). Isolation and purification of glycoglycerolipids to induce apoptosis in breast cancer cells. Scientific Reports, 11(1), 1298. https://doi.org/10.1038/s41598-020-80484-x Cite
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ACPA Decreases Non-Small Cell Lung Cancer Line Growth through Akt/PI3K and JNK Pathways In Vitro

Investigators confirmed that CB1 receptor (CB1R) was expressed in non-small cell lung cancer cells in this study. Arachidonoylcyclopropylamide (ACPA) as a synthetic, CB1R-specific ligand decreased proliferation rate in NSCLC cells by WST-1 analysis and real-time proliferation assay.
[Cell Death & Disease]
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Octenidine-Based Hydrogel Shows Anti-Inflammatory and Protease-Inhibitory Capacities in Wounded Human Skin

Proteomics analysis of octenidine dihydrochloride-treated skin wounds revealed significant lower levels of key players in tissue remodeling as well as reepithelization after wounding such as pro-inflammatory cytokines and matrix-metalloproteinases when compared to controls.
[Scientific Reports]
Seiser, S., Janker, L., Zila, N., Mildner, M., Rakita, A., Matiasek, J., Bileck, A., Gerner, C., Paulitschke, V., & Elbe-Bürger, A. (2021). Octenidine-based hydrogel shows anti-inflammatory and protease-inhibitory capacities in wounded human skin. Scientific Reports, 11(1), 32. https://doi.org/10.1038/s41598-020-79378-9 Cite
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Bile Accelerates Carcinogenic Processes in Pancreatic Ductal Adenocarcinoma Cells through the Overexpression of MUC4

Scientists caracterized the effect of bile acidson carcinogenic processes using PDAC cell lines and to investigate the underlying mechanisms. Liquid chromatography-mass spectrometry was used to determine the serum concentrations of bile acids.
[Scientific Reports]
Gál, E., Veréb, Z., Kemény, L., Rakk, D., Szekeres, A., Becskeházi, E., Tiszlavicz, L., Takács, T., Czakó, L., Hegyi, P., & Venglovecz, V. (2020). Bile accelerates carcinogenic processes in pancreatic ductal adenocarcinoma cells through the overexpression of MUC4. Scientific Reports, 10(1), 22088. https://doi.org/10.1038/s41598-020-79181-6 Cite
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