The generation of a tightly controlled ObLiGaRe doxycycline inducible SpCas9 (ODInCas9) transgene and its use in targeted ObLiGaRe resulted in functional integration into both human and mouse cells culminating in the generation of the ODInCas9 mouse.
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Lundin, A., Porritt, M. J., Jaiswal, H., Seeliger, F., Johansson, C., Bidar, A. W., Badertscher, L., Wimberger, S., Davies, E. J., Hardaker, E., Martins, C. P., James, E., Admyre, T., Taheri-Ghahfarokhi, A., Bradley, J., Schantz, A., Alaeimahabadi, B., Clausen, M., Xu, X., … Maresca, M. (2020). Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery. Nature Communications, 11(1), 4903. https://doi.org/10.1038/s41467-020-18548-9 Cite
A549 and H1299 cell lines were screened out and their parent cells and drug-resistant cells were co-cultured with human bone marrow mesenchymal stem cells-derived exosomes that had been transfected with miR-193a mimic or si-LRRC1 to detect the colony formation, migration, apoptosis, invasion and proliferation of non-small cell lung cancer cells.
[Cell Death & Disease]
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Wu, H., Mu, X., Liu, L., Wu, H., Hu, X., Chen, L., Liu, J., Mu, Y., Yuan, F., Liu, W., & Zhao, Y. (2020). Bone marrow mesenchymal stem cells-derived exosomal microRNA-193a reduces cisplatin resistance of non-small cell lung cancer cells via targeting LRRC1. Cell Death & Disease, 11(9), 1–14. https://doi.org/10.1038/s41419-020-02962-4 Cite
Researchers showed that increased fibroblast growth factor receptor 1 (FGFR1) promoted tumorigenic progression in precancerous neuroendocrine cells and is required for small cell lung cancer development in vivo.
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Non‐small cell lung cancer (NSCLC) is the leading cause of cancer death and in most cases it is often diagnosed at an advanced stage. Many genetic and microenvironmental factors are able to modify the cell cycle inducing carcinogenesis and tumor growth.
The authors identified a long noncoding RNA LINC00857 that might regulate radio-sensitivity of lung adenocarcinoma cells.
[Molecular Therapy-Nucleic Acids]
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The Janssen Pharmaceutical Companies of Johnson & Johnson announced interim results from the CHRYSALIS study, evaluating amivantamab, a fully human bispecific antibody that targets epidermal growth factor receptor (EGFR) and mesenchymal epithelial transition factor mutations, in combination with the third-generation EGFR tyrosine kinase inhibitor lazertinib in patients with non-small cell lung cancer with EGFR exon 19 deletions or L858R mutations.
[Janssen Global Services, LLC]
CB11 caused cell death via reactive oxygen species-mediated ATM-p53-GADD45α signaling in human non-small-cell lung cancer cells, and diphenyleneiodonium, an NADPH oxidase inhibitor, decreases cell death by inhibiting CB11-mediated ATM signaling.
[British Journal of Cancer]
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Investigators found that miR-196b-5p promoted lung cancer cell proliferation and colony formation by directly targeting tumor suppressor, FAS.
[Cell Death & Disease]
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A tumor xenograft mouse model was used to determine role of extracellular vesicles-microRNA-130b-3p and its target FOXO3 in lung cancer progression in vivo.
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Guo, Q., Yan, J., Song, T., Zhong, C., Kuang, J., Mo, Y., Tan, J., Li, D., Sui, Z., Cai, K., & Zhang, J. (2020). microRNA-130b-3p Contained in Mesenchymal Stem Cell-Derived Extracellular Vesicles Promotes Lung Cancer Progression by Regulating the FOXO3/NFE2L2/TXNRD1 Axis. Molecular Therapy - Oncolytics, 0(0). https://doi.org/10.1016/j.omto.2020.09.005 Cite
Scientists analyzed the anticancer potential of hmxbato against lung tumor cells, as well as the partial death mechanisms involved.
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In vitro assays on immortalized and patient-derived primary non-small cell lung cancer cells revealed that miR-34c-3p overexpression increased apoptosis and lowered proliferation rate in KRASmut cells.
[Cancer Gene Therapy]
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