macrophages

[Stem Cell Reports] Scientists described a defined, serum-free, open-source medium for the differentiation of iPSC-derived macrophages. This medium was equally capable of maintaining these cells compared with commercial alternatives.

[Cell Death & Disease] The pancreas was analyzed by histology, immunochemistry staining, and TUNEL assays, and the expression of certain proteins and RNAs, cytokine levels, trypsin activity, and reactive oxygen species (ROS) levels were determined.

[Xenotransplantation] The authors generated a rat xenotransplantation model to prove the role of macrophages as a xenotransplantation barrier. Immunodeficiency in SRG rats, which were Sprague–Dawley rats lacking Rag2 and Il2rg, was confirmed by flow cytometry and spleen immunostaining.

[Signal Transduction and Targeted Therapy] Aberrant bone marrow-M1/M2 MФ polarization, characterized by increased M1 MФs and decreased M2 MФs and accompanied by impaired megakaryopoiesis-supporting abilities, was found in patients with thrombocytopenia post-allotransplant.

[Nature Communications] Scientists used single-cell RNA sequencing analysis to delineate the immune landscape and tumor heterogeneity in a cohort of patients with HBV-associated human hepatocellular carcinoma.

[Science Advances] The authors evaluated effects of >50 million single-nucleotide polymorphisms and short insertions/deletions provided by five inbred strains of mice on the responses of macrophages to interleukin-4 (IL-4), a cytokine that plays pleiotropic roles in immunity and tissue homeostasis.