mass spectrometry

[Science Translational Medicine] To identify a protease signature of nonalcoholic steatohepatitis (NASH), transcriptomic analysis of 355 human liver biopsies identified a 13-protease panel that discriminated clinically relevant NASH ≥F2 fibrosis from F0-F1 with high classification accuracy across two independent patient datasets.

[Molecular Metabolism] Human duodenal organoids were CRISPR-Cas9 modified to express the fluorescent protein Venus or the Ca2+ sensor GCaMP7s under control of the endogenous motilin promoter.

[Journal of Translational Medicine] Investigators evaluated FBXO43 expression in breast cancer (BC) and analyzed The Cancer Genome Atlas. RT-qPCR and western blotting were utilized to detect FBXO43 expression in BC cell lines.

[Scientific Reports] Researchers profiled end-induction plasma, marrow, and cerebrospinal fluid (CSF) from ten children with b-cell acute lymphoblastic leukemia using liquid chromatography-mass spectrometry and observed plasma-marrow correlations for metabolites in pathways associated with end-induction residual disease and plasma-CSF correlations for a biomarker of fatigue.

[Nature Methods] Researchers studied the spatial metabolic profile in the human fibrotic liver. They discovered subpopulations of hepatocytes with special metabolic features associated with their proximity to the fibrotic niche, and validated this finding by spatial transcriptomics with Geo-seq.

[Molecular Cell] By combining Ribo-seq, mass spectrometry, and RNA-seq datasets, investigators discovered an oncomicropeptide, APPLE, encoded by a non-coding RNA transcript in acute myeloid leukemia.