Progressive and Coordinated Mobilization of the Skeletal Muscle Niche throughout Tissue Repair Revealed by Single-Cell Proteomic Analysis

Scientists developed a mass cytometry panel to investigate the dynamic nature of muscle regeneration at a single-cell level. Using the panel, they identified early changes in the proteome of stressed satellite and niche cells.
[Cells]
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Photobiomodulation Therapy Increases Collagen II after Tendon Experimental Injury

Photobiomodulation effectively interfered in tendon tissue repair after injury by stimulating mesenchymal cell proliferation and the synthesis of collagen type II, which was suggested to provide structural support to the interstitial tissues during the healing process of the Achilles tendon.
[Histology and Histopathology]
Photobiomodulation therapy increases collagen II after tendon experimental injury. (2021). Histology and Histopathology, 1. https://doi.org/10.14670/HH-18-330 Cite
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Isolating and Targeting the Real-Time Plasticity and Malignant Properties of Epithelial-Mesenchymal Transition in Cancer

3D high-content screening of ~23,000 compounds using dual-reporter mesenchymal SW620 tumor organoids identified small molecule probes that modulate EMT, and a subset of probes that effectively induced MET.
[Oncogene]
Esquer, H., Zhou, Q., Nemkov, T., Abraham, A. D., Rinaldetti, S., Chen, Y.-C., Zhang, X., Orman, M. V., D’Alessandro, A., Ferrer, M., Messersmith, W. A., & LaBarbera, D. V. (2021). Isolating and targeting the real-time plasticity and malignant properties of epithelial-mesenchymal transition in cancer. Oncogene, 1–14. https://doi.org/10.1038/s41388-021-01728-2 Cite
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Human Pluripotent Stem-Cell-Derived Alveolar Organoids for Modeling Pulmonary Fibrosis and Drug Testing

Scientists generated alveolar organoids derived from human pluripotent stem cells for use as an pulmonary fibrosis model and for drug efficacy evaluation.
[Cell Death Discovery]
Kim, J.-H., An, G. H., Kim, J.-Y., Rasaei, R., Kim, W. J., Jin, X., Woo, D.-H., Han, C., Yang, S.-R., Kim, J.-H., & Hong, S.-H. (2021). Human pluripotent stem-cell-derived alveolar organoids for modeling pulmonary fibrosis and drug testing. Cell Death Discovery, 7(1), 1–12. https://doi.org/10.1038/s41420-021-00439-7 Cite
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3D Mesenchymal Cell Migration Is Driven by Anterior Cellular Contraction That Generates an Extracellular Matrix Prestrain

The authors describe a cellular contractile mechanism employed by fibroblasts and mesenchymal cancer cells to migrate in 3D collagen gels.
[Developmental Cell]
Doyle, A. D., Sykora, D. J., Pacheco, G. G., Kutys, M. L., & Yamada, K. M. (2021). 3D mesenchymal cell migration is driven by anterior cellular contraction that generates an extracellular matrix prestrain. Developmental Cell, 0(0). https://doi.org/10.1016/j.devcel.2021.02.017 Cite
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USP34 Regulates Tooth Root Morphogenesis by Stabilizing NFIC

The authors showed that ubiquitin-specific protease 34 (USP34) plays a pivotal role in root formation. Deletion of Usp34 in dental mesenchymal cells led to short root anomaly, characterized by truncated roots and thin root dentin.
[International Journal of Oral Science]
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Culture and Analysis of Kidney Tubuloids and Perfused Tubuloid Cells-on-a-Chip

Scientists describe a detailed protocol for establishing tubuloid cultures from tissue and urine, as well as for generating and characterizing tubuloid cell–derived 3D tubular structures in a perfused microfluidic multi-chip platform.
[Nature Protocols]
Gijzen, L., Yousef Yengej, F. A., Schutgens, F., Vormann, M. K., Ammerlaan, C. M. E., Nicolas, A., Kurek, D., Vulto, P., Rookmaaker, M. B., Lanz, H. L., Verhaar, M. C., & Clevers, H. (2021). Culture and analysis of kidney tubuloids and perfused tubuloid cells-on-a-chip. Nature Protocols, 1–28. https://doi.org/10.1038/s41596-020-00479-w Cite
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Depletion of H3K36me2 Recapitulates Epigenomic and Phenotypic Changes Induced by the H3.3K36M Oncohistone Mutation

Researchers specifically depleted H3K36me2 or H3K36me3 in mesenchymal cells, using CRISPR-Cas9 to separately knock out the corresponding methyltransferases NSD1/2 or SETD2.
[Proceedings of the National Academy of Sciences of the United States of America]
Rajagopalan, K. N., Chen, X., Weinberg, D. N., Chen, H., Majewski, J., Allis, C. D., & Lu, C. (2021). Depletion of H3K36me2 recapitulates epigenomic and phenotypic changes induced by the H3.3K36M oncohistone mutation. Proceedings of the National Academy of Sciences, 118(9). https://doi.org/10.1073/pnas.2021795118 Cite
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PDGFRb+ Mesenchymal Cells, but not NG2+ Mural Cells, Contribute to Cardiac Fat

Scientists performed lineage tracing studies by using various genetic models and find that cardiac mesenchymal cells contribute to cardiac adipocytes in postnatal development and adult homeostasis.
[Cell Reports]
Jiang, Z., Feng, T., Lu, Z., Wei, Y., Meng, J., Lin, C.-P., Zhou, B., Liu, C., & Zhang, H. (2021). PDGFRb+ mesenchymal cells, but not NG2+ mural cells, contribute to cardiac fat. Cell Reports, 34(5). https://doi.org/10.1016/j.celrep.2021.108697 Cite
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SCA-1 Micro-Heterogeneity in the Fate Decision of Dystrophic Fibro/Adipogenic Progenitors

The authors suggest that micro-heterogeneity takes part in the fate decision of a mesenchymal cell population involved in the pathogenesis of a genetic disease.
[Cell Death & Disease]
Giuliani, G., Vumbaca, S., Fuoco, C., Gargioli, C., Giorda, E., Massacci, G., Palma, A., Reggio, A., Riccio, F., Rosina, M., Vinci, M., Castagnoli, L., & Cesareni, G. (2021). SCA-1 micro-heterogeneity in the fate decision of dystrophic fibro/adipogenic progenitors. Cell Death & Disease, 12(1), 1–24. https://doi.org/10.1038/s41419-021-03408-1 Cite
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Stromal DLK1 Promotes Proliferation and Inhibits Differentiation of the Intestinal Epithelium during Development

Scientists used a novel co-culture system with E14.5 intestinal epithelial organoids to examine the microenvironmental cues that regulate the embryonic stem/progenitor population, focusing on the role of Notch pathway factor, Delta-Like Protein 1.
[American Journal of Physiology-Gastrointestinal and Liver Physiology]
Ichinose, M., Suzuki, N., Wang, T., Wright, J. A., Lannagan, T. R., Vrbanac, L., Kobayashi, H., Gieniec, K. A., Ng, J. Q., Hayakawa, Y., García-Gallastegui, P., Monsalve, E. M., Bauer, S. R., Laborda, J., García-Ramírez, J. J., Ibarretxe, G., Worthley, D. L., & Woods, S. L. (2021). Stromal DLK1 promotes proliferation and inhibits differentiation of the intestinal epithelium during development. American Journal of Physiology-Gastrointestinal and Liver Physiology. https://doi.org/10.1152/ajpgi.00445.2020 Cite
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