Human articular chondrocytes and immortalised mesenchymal cells differentiated in chondrocytes were cultured in round-bottom 96-well plates to produce multicellular spheroids and their growth kinetics and viability was evaluated over seven days of culture.
[Tissue Engineering Part A]
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The authors identified growth hormone receptor is mainly expressed in mesenchymal cells, and its expression is substantially decreased in idiopathic pulmonary fibrosis lungs.
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Scientists used a human intestinal organoid model to define host transcriptional responses to S. Typhimurium infection, also determining host pathways dependent on Salmonella pathogenicity island- 1- and -2-encoded type 3 secretion systems
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Lawrence, A.-L. E., Abuaita, B. H., Berger, R. P., Hill, D. R., Huang, S., Yadagiri, V. K., Bons, B., Fields, C., Wobus, C. E., Spence, J. R., Young, V. B., & O’Riordan, M. X. (2021). Salmonella enterica Serovar Typhimurium SPI-1 and SPI-2 Shape the Global Transcriptional Landscape in a Human Intestinal Organoid Model System. MBio, 12(3). https://doi.org/10.1128/mBio.00399-21 Cite
Researchers completed scRNA-seq analysis of mesenchymal cells from the embryonic, post-natal, adult and aged fibrotic lungs of mice and humans.
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Liu, X., Rowan, S. C., Liang, J., Yao, C., Huang, G., Deng, N., Xie, T., Wu, D., Wang, Y., Burman, A., Parimon, T., Borok, Z., Chen, P., Parks, W. C., Hogaboam, C. M., Weigt, S. S., Belperio, J., Stripp, B. R., Noble, P. W., & Jiang, D. (2021). Categorization of Lung Mesenchymal Cells in Development and Fibrosis. IScience, 0(0). https://doi.org/10.1016/j.isci.2021.102551 Cite
The authors reported that after distinct types of intestinal epithelial damage, IL-1R1 signaling in GREM1+ mesenchymal cells increased production of R-spondin 3, a Wnt agonist required for intestinal stem cell self-renewal.
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Using ChIP-Seq against NACA in parallel with RNA-sequencing, investigators report the identification of Ubiquitin Specific Peptidase 53 (Usp53) as a target gene of parathyroid hormone-activated nascent polypeptide associated complex and coregulator alpha in osteoblasts.
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Scientists developed a mass cytometry panel to investigate the dynamic nature of muscle regeneration at a single-cell level. Using the panel, they identified early changes in the proteome of stressed satellite and niche cells.
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Photobiomodulation effectively interfered in tendon tissue repair after injury by stimulating mesenchymal cell proliferation and the synthesis of collagen type II, which was suggested to provide structural support to the interstitial tissues during the healing process of the Achilles tendon.
[Histology and Histopathology]
3D high-content screening of ~23,000 compounds using dual-reporter mesenchymal SW620 tumor organoids identified small molecule probes that modulate EMT, and a subset of probes that effectively induced MET.
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Esquer, H., Zhou, Q., Nemkov, T., Abraham, A. D., Rinaldetti, S., Chen, Y.-C., Zhang, X., Orman, M. V., D’Alessandro, A., Ferrer, M., Messersmith, W. A., & LaBarbera, D. V. (2021). Isolating and targeting the real-time plasticity and malignant properties of epithelial-mesenchymal transition in cancer. Oncogene, 1–14. https://doi.org/10.1038/s41388-021-01728-2 Cite
Scientists generated alveolar organoids derived from human pluripotent stem cells for use as an pulmonary fibrosis model and for drug efficacy evaluation.
[Cell Death Discovery]
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Kim, J.-H., An, G. H., Kim, J.-Y., Rasaei, R., Kim, W. J., Jin, X., Woo, D.-H., Han, C., Yang, S.-R., Kim, J.-H., & Hong, S.-H. (2021). Human pluripotent stem-cell-derived alveolar organoids for modeling pulmonary fibrosis and drug testing. Cell Death Discovery, 7(1), 1–12. https://doi.org/10.1038/s41420-021-00439-7 Cite
The authors describe a cellular contractile mechanism employed by fibroblasts and mesenchymal cancer cells to migrate in 3D collagen gels.
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