monocytes

[Scientific Reports] Researchers investigated the impact of IL-27 in both autophagy induction and HIV-1 infection in macrophages.

[Scientific Reports] The authors identified an important caveat when investigating TLR4-driven signaling in BLaER1 cells. They showed that this model contained glycosylphosphatidylinositol anchor-deficient cells, which lacked CD14 surface expression when differentiated to monocytes, resulting in diminished LPS/TLR4 but not TLR7/TLR8 responsiveness.

[Molecular Therapy-Methods & Clinical Development] Investigators found that high CD14+ cell content posed a challenge for manufacturing CAR T cells especially in patients with non-Hodgkin’s lymphoma and multiple myeloma due to the non-specific phagocytosis of the magnetic beads used to activate CD3+ T cells.

[Journal of Leukocyte Biology] miRNA profiles were evaluated and eight miRNAs were found to be differentially expressed in patient-derived cell lines in comparison to controls. miR-181c was predicted to target myeloid cell leukemia (MCL)1, which was confirmed by transfection assays, resulting in significantly reduced MCL1 mRNA and decreased live cell numbers.

[npj Parkinson's Disease] The authors reported the development of a highly sensitive and reproducible Bio-ELISA to quantify picogram levels of TH in multiple model systems. They applied this assay to monocytes isolated from blood of persons with Parkinson’s disease and to age-matched, healthy controls.

[Translational Psychiatry] Researchers examined whether retrospective reports of adversity during childhood or pregnancy were associated with inflammatory imbalance, in an ethnically diverse cohort of 53 low-income women seeking family-based trauma treatment following exposure to interpersonal violence.