Isolation of Murine Bone Marrow by Centrifugation or Flushing for the Analysis of Hematopoietic Cells – A Comparative Study

Researchers adapted a centrifugation-based method for the isolation of murine bone marrow and compared it to the traditional flushing method. Analysis of primary hematopoietic stem cells, immune cells, and megakaryocytes revealed a comparable distribution of cellular (sub)populations.
[Platelets]
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Specific Targeting of Ovarian Tumor-Associated Macrophages by Large, Anionic Nanoparticles

Researchers showed that relatively large anionic nanoparticles administered intraperitoneally selectively accumulated in tumor-associated macrophages.
[Proceedings of the National Academy of Sciences of the United States of America]
Haber, T., Cornejo, Y. R., Aramburo, S., Flores, L., Cao, P., Liu, A., Mooney, R., Gilchrist, M., Tirughana, R., Nwokafor, U., Abidi, W., Han, E., Dellinger, T., Wakabayashi, M. T., Aboody, K. S., & Berlin, J. M. (2020). Specific targeting of ovarian tumor-associated macrophages by large, anionic nanoparticles. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.1917424117 Cite
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Targeting QKI-7 In Vivo Restores Endothelial Cell Function in Diabete

QKI-7 is highly expressed in human coronary arterial endothelial cells from diabetic donors, and on blood vessels from diabetic critical limb ischemia patients undergoing a lower-limb amputation.
[Nature Communications]
Yang, C., Eleftheriadou, M., Kelaini, S., Morrison, T., González, M. V., Caines, R., Edwards, N., Yacoub, A., Edgar, K., Moez, A., Ivetic, A., Zampetaki, A., Zeng, L., Wilkinson, F. L., Lois, N., Stitt, A. W., Grieve, D. J., & Margariti, A. (2020). Targeting QKI-7 in vivo restores endothelial cell function in diabetes. Nature Communications, 11(1), 3812. https://doi.org/10.1038/s41467-020-17468-y Cite
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Alteration of Immunophenotype of Human Macrophages and Monocytes after Exposure to Cigarette Smoke

Using human monocyte-derived macrophages (MDMs) from healthy donors, researchers found that cigarette smoke exposure was not associated with significant changes in the production of pro inflammatory cytokines by MDMs.
[Scientific Reports]
da Silva, C. O., Gicquel, T., Daniel, Y., Bártholo, T., Vène, E., Loyer, P., Pôrto, L. C., Lagente, V., & Victoni, T. (2020). Alteration of immunophenotype of human macrophages and monocytes after exposure to cigarette smoke. Scientific Reports, 10(1), 12796. https://doi.org/10.1038/s41598-020-68753-1 Cite
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Histone Deacetylase 3 Controls Lung Alveolar Macrophage Development and Homeostasis

Researchers showed that histone deacetylase 3 was a key epigenetic factor required for avelolar macrophage embryonic development, postnatal homeostasis, maturation, and regeneration from bone marrow.
[Nature Communications]
Yao, Y., Liu, Q., Adrianto, I., Wu, X., Glassbrook, J., Khalasawi, N., Yin, C., Yi, Q., Dong, Z., Geissmann, F., Zhou, L., & Mi, Q.-S. (2020). Histone deacetylase 3 controls lung alveolar macrophage development and homeostasis. Nature Communications, 11(1), 3822. https://doi.org/10.1038/s41467-020-17630-6 Cite
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Increased CXCL4 Expression in Hematopoietic Cells Links Inflammation and Progression of Bone Marrow Fibrosis in MPN

The authors demonstrated that the absence of hematopoietic CXCL4 ameliorates the myeloproliferative neoplasm phenotype, reduces stromal cell activation and bone marrow fibrosis and decreases the activation of pro-fibrotic pathways in megakaryocytes, inflammation in fibrosis-driving cells and JAK/STAT activation in both megakaryocytes and stromal cells in three murine primary myelofibrosis models.
[Blood]
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Combination Product of Dermal Matrix, Human Mesenchymal Stem Cells, and Timolol Promotes Diabetic Wound Healing in Mice

Scientists investigated whether a bioengineered scaffold device containing hypoxia‐preconditioned, allogeneic human mesenchymal stem cells combined with the beta‐adrenergic antagonist timolol could improve impaired wound healing in diabetic mice.
[Stem Cells Translational Medicine]
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An Apoptotic Caspase Network Safeguards Cell Death Induction in Pyroptotic Macrophages

The authors showed that independently of GSDMD-mediated plasma membrane permeabilization, inflammasome receptors engaged caspase-1 and caspase-8, both of which redundantly promoted activation of apoptotic executioner caspase-3 and caspase-7 in pyroptotic macrophages.
[Cell Reports]
Vasconcelos, N. M. de, Opdenbosch, N. V., Gorp, H. V., Martín-Pérez, R., Zecchin, A., Vandenabeele, P., & Lamkanfi, M. (2020). An Apoptotic Caspase Network Safeguards Cell Death Induction in Pyroptotic Macrophages. Cell Reports, 32(4). https://doi.org/10.1016/j.celrep.2020.107959 Cite
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The Soluble Glycoprotein NMB (GPNMB) Produced by Macrophages Induces Cancer Stemness and Metastasis via CD44 and IL-33

Using mouse tumor models, the authors showed that macrophage-derived soluble glycoprotein nonmetastatic B (GPNMB) increased tumor growth and metastasis in Gpnmb-mutant mice.
[Cellular & Molecular Immunology]
Liguori, M., Digifico, E., Vacchini, A., Avigni, R., Colombo, F. S., Borroni, E. M., Farina, F. M., Milanesi, S., Castagna, A., Mannarino, L., Craparotta, I., Marchini, S., Erba, E., Panini, N., Tamborini, M., Rimoldi, V., Allavena, P., & Belgiovine, C. (2020). The soluble glycoprotein NMB (GPNMB) produced by macrophages induces cancer stemness and metastasis via CD44 and IL-33. Cellular & Molecular Immunology, 1–12. https://doi.org/10.1038/s41423-020-0501-0 Cite
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Neutrophil Caspase-11 Is Essential to Defend against a Cytosol-Invasive Bacterium

Although both caspase-1 and caspase-11 could cleave gasdermin D in macrophages and neutrophils, researchers found that NLRC4-activated caspase-1 triggered pyroptosis in macrophages, but this pathway did not trigger pyroptosis in neutrophils.
[Cell Reports]
Kovacs, S. B., Oh, C., Maltez, V. I., McGlaughon, B. D., Verma, A., Miao, E. A., & Aachoui, Y. (2020). Neutrophil Caspase-11 Is Essential to Defend against a Cytosol-Invasive Bacterium. Cell Reports, 32(4). https://doi.org/10.1016/j.celrep.2020.107967 Cite
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Effect of Simulated Microgravity Conditions of Hindlimb Unloading on Mice Hematopoietic and Mesenchymal Stromal Cells

C57BL/6 mice were randomly divided into two groups: a control group and a hindlimb suspension group. After four weeks of hindlimb suspension, scientists found that this simulated microgravity condition increased the percentage of monocytes and macrophages and decreased the percentage of B lymphocytes and mature red cells in bone marrow.
[Cell Biology International]
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