Brain organoids derived from iPSCs of patients are a powerful avenue to investigate the pathophysiological processes. Scientists generated iPSC-derived cerebral organoids from monozygotic twins discordant for psychosis.
6630899 UM7CBM6R items 1 apa default asc 1
Sawada, T., Chater, T. E., Sasagawa, Y., Yoshimura, M., Fujimori-Tonou, N., Tanaka, K., Benjamin, K. J. M., Paquola, A. C. M., Erwin, J. A., Goda, Y., Nikaido, I., & Kato, T. (2020). Developmental excitation-inhibition imbalance underlying psychoses revealed by single-cell analyses of discordant twins-derived cerebral organoids. Molecular Psychiatry, 1–17. https://doi.org/10.1038/s41380-020-0844-z Cite
Investigators established anti-AQP4 recombinant autoantibodies from plasmablasts in neuromyelitis optica spectrum disorder patient’s cereberospinal fluid.
Scientists present an experimental system based on 3D scaffold-based cortical neuron cultures in which they expressed the genetically encoded calcium indicator GCaMP6f to record neuronal activity at the millimeter-scale.
6630899 SMQR5JPU items 1 apa default asc 1
Functional characterization of three-dimensional cortical cultures for in vitro modeling of brain networks: iScience. (n.d.). Retrieved August 6, 2020, from https://www.cell.com/iscience/fulltext/S2589-0042(20)30624-6 Cite
Researchers generated distinct imageable syngeneic mouse glioblastoma-tumor models and utilized RNA-sequencing, CyTOF and correlative immunohistochemistry to assess immune-profiles in these models.
6445212 I68LG74H items 1 apa default asc 1
Scientists showed that notch signaling-deficient astrocytes could generate new neurons after injury. Using single-cell RNA sequencing, they found that, when notch signaling was blocked, astrocytes transitioned to a neural stem cell-like state.
[Cell Stem Cell]
6606345 Y694T5X9 items 1 apa default asc 1
Researchers used single-cell RNA sequencing to characterize neurogenesis by notch-depleted striatal astrocytes in vivo.
6606345 78HF3Y8V items 1 apa default asc 1
Research conducted over the past 10 years has revealed that cortical GABAergic projection neurons are highly diverse in terms of molecular marker expression, synaptic targeting, activity pattern during distinct behavioural states and precise temporal recruitment relative to ongoing neuronal network oscillations.
[Nature Reviews Neuroscience]
6606345 P5WUWYE3 items 1 apa default asc 1
The authors investigated the role of V1G1 in glioma stem cell small extracellular vesicles and their effects on primary brain cultures.
[Molecular Cancer Research]
1254621 ZLJ8M46F items 1 apa default asc 1
Investigators demonstrated that human midbrain organoids showed essential neuronal functional properties as spontaneous electrophysiological activity of different neuronal subtypes, including dopaminergic, GABAergic, glutamatergic and serotonergic neurons.
[Cell and Tissue Research]
6445212 KYFPRAKT items 1 apa default asc 1
Smits, L. M., Magni, S., Kinugawa, K., Grzyb, K., Luginbühl, J., Sabate-Soler, S., Bolognin, S., Shin, J. W., Mori, E., Skupin, A., & Schwamborn, J. C. (2020). Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids. Cell and Tissue Research. https://doi.org/10.1007/s00441-020-03249-y Cite
Neural ribbons offer multifunctionality in vitro including co-encapsulation of the injury site ESM modifier chondroitinase ABC, tested in glial scar models, and ability of cervically-patterned spinal cord neural stem cells to differentiate within neural ribbons and project axons for integration with 3D external matrices.
6630899 SUNYGVIN items 1 apa default asc 1
Olmsted, Z. T., Stigliano, C., Badri, A., Zhang, F., Williams, A., Koffas, M. A. G., Xie, Y., Linhardt, R. J., Cibelli, J., Horner, P. J., & Paluh, J. L. (2020). Fabrication of homotypic neural ribbons as a multiplex platform optimized for spinal cord delivery. Scientific Reports, 10(1), 12939. https://doi.org/10.1038/s41598-020-69274-7 Cite
Scientists investigated immunomodulatory potential of human Wharton’s jelly mesenchymal stem cells derived extracellular vesicles on inflammasome activity one week after spinal cord injury in rats.
1254621 CH8SZ5IJ items 1 apa default asc 1