A Cis-Acting Structural Variation at the ZNF558 Locus Controls a Gene Regulatory Network in Human Brain Development

Scientists identified a KRAB-ZFP transcription factor, ZNF558, that was expressed in human but not chimpanzee forebrain neural progenitor cells.
[Cell Stem Cell]
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A Polycomb Domain Found in Committed Cells Impairs Differentiation When Introduced into PRC1 in Pluripotent Cells

ESCs expressing a chimeric CBX7 were impaired in their ability to properly form embryoid bodies and neural progenitor cells and showed reduced activation of lineage-specific genes across differentiation.
[Molecular Cell]
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Cend1 and Neurog2 Efficiently Reprogram Human Cortical Astrocytes to Neural Precursor Cells and Induced-Neurons

Scientists investigated the neuronal reprogramming capacity of CEND1 and/or Neurogenin-2 (NEUROG2) upon their overexpression on primary human adult cortical astrocytes.
[International Journal of Developmental Biology]
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Robust Neuronal Differentiation of Human iPSC-Derived Neural Progenitor Cells Cultured on Densely-Spaced Spiky Silicon Nanowire Arrays

Researchers presented human patient induced pluripotent stem cell-derived neurons cultured on densely-spaced spiky silicon nanowire arrays which showed mature electrophysiological characteristics after only 20 days of culturing.
[Scientific Reports]
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High-Content Image-Based Analysis and Proteomic Profiling Identifies Tau Phosphorylation Inhibitors in a Human iPSC-Derived Glutamatergic Neuronal Model of Tauopathy

Scientists used iPSCs from a patient carrying a p.A152T tau variant to create a robust, functional cellular assay system for probing pathophysiological tau accumulation and phosphorylation.
[Scientific Reports]
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CTCF Is a Barrier for 2C-Like Reprogramming

Researchers showed that the CCCTC-binding factor (CTCF) was a barrier for 2C-like reprogramming. Depletion of CTCF in ESCs efficiently promoted spontaneous and asynchronous conversion to a 2C-like state and was reversible upon restoration of CTCF levels.
[Nature Communications]
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Exposure to Cadmium Induces Neuroinflammation and Impairs Ciliogenesis in hESC-Derived 3D Cerebral Organoids

The authors identified that cadmium exposure specifically induced apoptosis in neurons and inhibited the proliferation of neural progenitor cells, but neural differentiation was not significantly affected in cerebral organoids.
[Science of the Total Environment]
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Developing Human Pluripotent Stem Cell-Based Cerebral Organoids with a Controllable Microglia Ratio for Modeling Brain Development and Pathology

Investigators reported a new brain region-specific, microglia-containing organoid model by co-culturing human PSC-derived primitive neural progenitor cells and primitive macrophage progenitors.
[Stem Cell Reports]
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Deletion of RBMX RGG/RG Motif in Shashi-XLID Syndrome Leads to Aberrant p53 Activation and Neuronal Differentiation Defects

Scientists investigated the contribution of the X-linked intellectual disability (XLID)-associated RBMX in neuronal differentiation.Transcriptomic analysis of isogenic Shashi-XLID human iPSCs generated using CRISPR-Cas9 revealed a dysregulation of MDM4 splicing and aberrant p53 upregulation.
[Cell Reports]
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Generation of Caudal-Type Serotonin Neurons and Hindbrain-Fate Organoids from hPSCs

Scientists proposed a novel strategy to generate hindbrain serotonin (5-HT) neurons from human PSCs, which involved the formation of ventral-type neural progenitor cells and stimulation of the hindbrain 5-HT neural development.
[Stem Cell Reports]
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Hypoproliferative Human Neural Progenitor Cell Xenografts Survived Extendedly in the Brain of Immunocompetent Rats

Neuron-restricted neural progenitor cells (NPCs) were derived from human embryonic stem cells, propagated for different passages, and then transplanted into the brain of immunocompetent rats without immunosuppressants.
[Stem Cell Research & Therapy]
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