Single-Cell Transcriptomics of Human iPSC Differentiation Dynamics Reveal a Core Molecular Network of Parkinson’s Disease

Researchers investigated Parkinson’s disease (PD)’s mechanism using dopaminergic neurons differentiated from human iPSCs carrying the ILE368ASN mutation within the PINK1 gene, which is strongly associated with PD.
[Communications Biology]
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Stretchable Mesh Nanoelectronics for Three-Dimensional Single-Cell Chronic Electrophysiology from Developing Brain Organoids

The authors reported a cyborg brain organoid platform, in which “tissue-like” stretchable mesh nanoelectronics are designed to match the mechanical properties of brain organoids and to be folded by the organogenetic process of progenitor or stem cells, distributing stretchable electrode arrays across the 3D organoids.
[Advanced Materials]
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Effects of Intracellular Calcium Accumulation on Proteins Encoded by the Major Genes Underlying Amyotrophic Lateral Sclerosis

Researchers aimed to find a relationship between the proteins coded by the most relevant genes associated with amyotrophic lateral sclerosis and intracellular Ca2+ accumulation.
[Scientific Reports]
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Engineering Brain Assembloids to Interrogate Human Neural Circuits

Scientists presented how to generate 3D spheroids resembling specific domains of the nervous system and then how to integrate them physically to allow axonal projections and synaptic assembly.
[Nature Protocols]
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Organization and Dynamics of the Cortical Complexes Controlling Insulin Secretion in β-Cells

Researchers showed that insulin secretion sites also contain non-neuronal proteins LL5β and KANK1, which in migrating cells organize exocytotic machinery in the vicinity of integrin-based adhesions.
[Journal of Cell Science]
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JIP3 Links Lysosome Transport to Regulation of Multiple Components of the Axonal Cytoskeleton

Building on observations that mutations in the JIP3 gene result in lysosome-filled axonal swellings, scientists analyzed the impact of JIP3 depletion on the cytoskeleton of human neurons.
[Communications Biology]
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Lineage Recording in Human Cerebral Organoids

Scientists established iTracer, a lineage recorder that combined reporter barcodes with inducible CRISPR–Cas9 scarring and was compatible with single-cell and spatial transcriptomics.
[Nature Methods]
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Making Neurons, Made Easy: The Use of Neurogenin-2 in Neuronal Differentiation

Directed neuronal differentiation of human PSCs, neural progenitors, or fibroblasts using transcription factors has allowed for the rapid and highly reproducible differentiation of mature and functional neurons.
[Stem Cell Reports]
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Establishment of Human Pluripotent Stem Cell-Derived Skin Organoids Enabled Pathophysiological Model of SARS-CoV-2 Infection

A proteome map of COVID-19 skin was established and the susceptibility of human-induced pluripotent stem cell-derived skin organoids with hair follicles and nervous system was investigated, to SARS-CoV-2 infection.
[Advanced Science]
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KIF5C Deficiency Causes Abnormal Cortical Neuronal Migration, Dendritic Branching, and Spine Morphology in Mice

Investigators observed high KIF5C expression in neurons during the early developmental stage in mice and the human brain. Kif5c deficiency resulted in disturbed cortical neuronal migration, dendritic, and spine growth.
[Pediatric Research]
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EGF Downregulates Presynaptic Maturation and Suppresses Synapse Formation In Vitro and In Vivo

Researchers showed that epidermal growth factor (EGF) negatively regulated presynaptic maturation and synapse formation. In cortical neurons, EGF maintained axon elongation and reduced the sizes of growth cones in culture.
[Neurochemical Research]
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