Canonical TGFβ Signaling Induces Collective Invasion in Colorectal Carcinogenesis through a Snail1- and Zeb1-Independent Partial EMT

The authors investigated the impact of oncogenic transformation and the microenvironment on tumor cell invasion using genetically engineered organoids as colorectal cancer models.
[Oncogene]
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Midbrain Organoids Mimic Early Embryonic Neurodevelopment and Recapitulate LRRK2-p.Gly2019Ser-Associated Gene Expression

The authors compared single-cell RNA-sequencing data of human midbrain organoids to the developing human embryonic midbrain.
[American Journal of Human Genetics]
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Reaching into the Toolbox: Stem Cell Models to Study Neuropsychiatric Disorders

Investigators discussed available tools, including CRISPR-Cas9, and the applications of these tools to develop cell-based 2D models and 3D brain organoid models that better represent and unravel the intricacies of neuropsychiatric disorder pathophysiology.
[Stem Cell Reports]
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Recapitulating Hepatitis E Virus–Host Interactions and Facilitating Antiviral Drug Discovery in Human Liver–Derived Organoids

Scientists demonstrated that 3D cultured organoids from fetal and adult human liver with cholangiocyte or hepatocyte phenotype supported hepatitis E virus replication.
[Science Advances]
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Expression of the Transcription Factor PU.1 Induces the Generation of Microglia-Like Cells in Human Cortical Organoids

The authors developed a method to generate functional microglia in human cortical organoids from human embryonic stem cells.
[Nature Communications]
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Anti-miR-135/SPOCK1 Axis Antagonizes the Influence of Metabolism on Drug Response in Intestinal/Colon Tumor Organoids

The authors analyzed the expression of 373 miRNAs and 12 deregulated metabolic genes in murine duodenal polyps and non-polyp-derived organoids.
[Oncogenesis]
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RNF43/ZNRF3 Loss Predisposes to Hepatocellular-Carcinoma by Impairing Liver Regeneration and Altering the Liver Lipid Metabolic Ground-State

Using hepatocyte-, hepatoblast- and ductal cell-derived organoids, investigators demonstrated that the differentiation defects and lipid alterations were, in part, cell-autonomous.
[Nature Communications]
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Ex Vivo and In Vivo Suppression of SARS-CoV-2 with Combinatorial AAV-RNAi Expression Vectors

Researchers comprehensively illustrated the capacity of adeno-associated virus (AAV) vectors co-expressing a cocktail of three short hairpin RNAs directed against the SARS-CoV-2 RdRp and N genes as versatile and effective antivirals.
[Molecular Therapy]
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GSDMB Is Increased in IBD and Regulates Epithelial Restitution/Repair Independent of Pyroptosis

The authors reported increased gasdermin B (GSDMB) in inflammatory bowel disease, with single-cell analysis identifying epithelial specificity to inflamed colonocytes/crypt top colonocytes.
[Cell]
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Development of Human Alveolar Epithelial Cell Models to Study Distal Lung Biology and Disease

Researchers used de-identified human remnant transplant lungs to develop a method to establish alveolar epithelial cell lines. The lines grew well in 2D culture as epithelial monolayers expressing lung progenitor markers.
[iScience]
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PAX6-Positive Microglia Evolve Locally in hiPSC-Derived Ocular Organoids

Investigators studied human iPSCs that had been expanded into a self-formed ectodermal autonomous multi-zone of cells that partially mimicked human eye development.
[Stem Cell Reports]
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