Prostate Cancer Patient-Derived Organoids: Detailed Outcome from a Prospective Cohort of 81 Clinical Specimens

Investigators report on efforts to generate patient‐derived organoids from a cohort of 81 prostate cancer specimens with diverse pathological and clinical features. They comprehensively analyzed histological features of each enrolled sample and correlated it with organoid growth patterns.
[Journal of Pathology]
Servant, R., Garioni, M., Vlajnic, T., Blind, M., Pueschel, H., Müller, D. C., Zellweger, T., Templeton, A. J., Garofoli, A., Maletti, S., Piscuoglio, S., Rubin, M. A., Seifert, H., Rentsch, C. A., Bubendorf, L., & Magnen, C. L. (n.d.). Prostate cancer patient-derived organoids: detailed outcome from a prospective cohort of 81 clinical specimens. The Journal of Pathology, n/a(n/a). https://doi.org/https://doi.org/10.1002/path.5698 Cite
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Primate Organoids and Gene-Editing Technologies toward Next-Generation Biomedical Research

Advances in gene-editing technologies, particularly CRISPR-mediated techniques, offer tremendous potential in facilitating organoid research, including the study of development, disease modeling, and personalized medicine.
[Trends in Biotechnology]
Chen, C., Ji, W., & Niu, Y. (2021). Primate Organoids and Gene-Editing Technologies toward Next-Generation Biomedical Research. Trends in Biotechnology, 0(0). https://doi.org/10.1016/j.tibtech.2021.03.010 Cite
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Early Response in Phosphorylation of Ribosomal Protein S6 Is Associated with Sensitivity to Trametinib in Colorectal Cancer Cells

The authors investigated the value of the response in pS6 for predicting the efficacy of trametinib, a MEK inhibitor, in patients with RAS/BRAF mutant colorectal cancer (CRC) using patient-derived CRC organoids.
[Laboratory Investigation]
Hirashita, Y., Tsukamoto, Y., Kudo, Y., Kakisako, D., Kurogi, S., Hijiya, N., Nakada, C., Uchida, T., Hirashita, T., Hiratsuka, T., Akagi, T., Ueda, Y., Shiroshita, H., Etoh, T., Mizukami, K., Honda, K., Okimoto, T., Kodama, M., Inomata, M., … Murakami, K. (2021). Early response in phosphorylation of ribosomal protein S6 is associated with sensitivity to trametinib in colorectal cancer cells. Laboratory Investigation, 1–12. https://doi.org/10.1038/s41374-021-00590-w Cite
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Inflation-Collapse Dynamics Drive Patterning and Morphogenesis in Intestinal Organoids

The authors examined processes governing the size of stem cell zones. They improved the viability and homogeneity of intestinal organoid cultures to enable long-term time-lapse imaging of multiple organoids in parallel.
[Cell Stem Cell]
Tallapragada, N. P., Cambra, H. M., Wald, T., Jalbert, S. K., Abraham, D. M., Klein, O. D., & Klein, A. M. (2021). Inflation-collapse dynamics drive patterning and morphogenesis in intestinal organoids. Cell Stem Cell, 0(0). https://doi.org/10.1016/j.stem.2021.04.002 Cite
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Win, Lose, or Tie: Mathematical Modeling of Ligand Competition at the Cell–Extracellular Matrix Interface

Toward the goal of fully functional organoid development, the authors hypothesized that resolving the dynamics of extracellular matrix–integrin interactions would be highly instructive. To this end, they developed a mathematical model that enabled then to simulate three main interactions, namely integrin activation, ligand binding, and integrin clustering.
[Frontiers in Bioengineering and Biotechnology]
Karagöz, Z., Geuens, T., LaPointe, V. L. S., van Griensven, M., & Carlier, A. (2021). Win, Lose, or Tie: Mathematical Modeling of Ligand Competition at the Cell–Extracellular Matrix Interface. Frontiers in Bioengineering and Biotechnology, 9. https://doi.org/10.3389/fbioe.2021.657244 Cite
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Functional Characterization of Organoids Derived from Irreversibly Damaged NASH Patient Liver

After hepatic differentiation, NASH liver organoids exhibited reduced ability to dedifferentiate back to the biliary state, consistent with the known reduced regenerative ability of NASH livers.
[Hepatology]
McCarron, S., Bathon, B., Conlon, D. M., Abbey, D., Rader, D. J., Gawronski, K., Brown, C. D., Olthoff, K. M., Shaked, A., & Raabe, T. D. (n.d.). Functional characterization of organoids derived from irreversibly damaged NASH patient liver. Hepatology, n/a(n/a). https://doi.org/https://doi.org/10.1002/hep.31857 Cite
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HER2 and APC Mutations Promote Altered Crypt-Villus Morphology and Marked Hyperplasia in the Intestinal Epithelium

Investigators analyzed Lgr5-Cre; APCmin; HER2V777LTg mice by morphologic and gene expression assays on intestinal sections and organoids derived from the epithelium.
[Cellular and Molecular Gastroenterology and Hepatology]
Murray, E., Cheng, X., Krishna, A., Jin, X., Ohara, T. E., Stappenbeck, T. S., & Bose, R. (2021). HER2 and APC mutations promote altered crypt-villus morphology and marked hyperplasia in the intestinal epithelium. Cellular and Molecular Gastroenterology and Hepatology. https://doi.org/10.1016/j.jcmgh.2021.04.012 Cite
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Commitment and Oncogene-Induced Plasticity of Human Stem Cell-Derived Pancreatic Acinar and Ductal Organoids

Scientists report induction of human pluripotent stem cells toward pancreatic ductal and acinar organoids that recapitulate properties of the neonatal exocrine pancreas.
[Cell Stem Cell]
Huang, L., Desai, R., Conrad, D. N., Leite, N. C., Akshinthala, D., Lim, C. M., Gonzalez, R., Muthuswamy, L. B., Gartner, Z., & Muthuswamy, S. K. (2021). Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids. Cell Stem Cell, 0(0). https://doi.org/10.1016/j.stem.2021.03.022 Cite
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Epigenetic Regulation during Human Cortical Development: Seq-ing Answers from the Brain to the Organoid

The authors examine recent studies that have defined changes in the epigenomic landscape during fetal brain development. They compare these studies with analogous data derived by in vitro differentiation of hPSCs into specific neuronal cell types or as 3D cerebral organoids.
[Neurochemistry International]
Lewis, E. M. A., Kaushik, K., Sandoval, L. A., Antony, I., Dietmann, S., & Kroll, K. L. (2021). Epigenetic regulation during human cortical development: seq-ing answers from the brain to the organoid. Neurochemistry International, 105039. https://doi.org/10.1016/j.neuint.2021.105039 Cite
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Modeling Plasticity and Dysplasia of Pancreatic Ductal Organoids Derived from Human Pluripotent Stem Cells

Researchers differentiate hPSCs into pancreatic duct-like organoids (PDLOs) with morphological, transcriptional, proteomic, and functional characteristics of human pancreatic ducts, further maturing upon transplantation into mice.
[Cell Stem Cell]
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Generating Human Prostate Cancer Organoids from Leukapheresis Enriched Circulating Tumor Cells

Scientists used diagnostic leukapheresis to enrich circulating tumor cells from patients with metastatic prostate cancer and explored whether organoids provide a platform for ex vivo treatment modeling.
[European Journal of Cancer]
Mout, L., van Dessel, L. F., Kraan, J., de Jong, A. C., Neves, R. P. L., Erkens-Schulze, S., Beaufort, C. M., Sieuwerts, A. M., van Riet, J., Woo, T. L. C., de Wit, R., Sleijfer, S., Hamberg, P., Sandberg, Y., te Boekhorst, P. A. W., van de Werken, H. J. G., Martens, J. W. M., Stoecklein, N. H., van Weerden, W. M., & Lolkema, M. P. (2021). Generating human prostate cancer organoids from leukapheresis enriched circulating tumour cells. European Journal of Cancer, 150, 179–189. https://doi.org/10.1016/j.ejca.2021.03.023 Cite
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