organoids

[European Respiratory Journal] Individual cystic fibrosis transmembrane conductance regulator function was defined by forskolin-induced swelling, measured as the relative size increase of intestinal organoids after stimulation with 0.8 µM forskolin, quantified as area under the curve.

[eLife] Researchers investigated the dynamics of basement membrane assembly in human pluripotent stem cell-derived kidney organoids.

[Science Advances] Six candidates among the most commonly shared HLA-A2 epitopes with evidence of translation were selected for immunological evaluation. In vitro priming assays confirmed the immunogenicity of these epitopes, which induced high-avidity CD8+ T cell clones.

[American Journal of Physiology-Lung Cellular and Molecular Physiology] Investigators described a simple method to generate organoid cultures using isolated human tracheobronchial epithelial cells grown in mixed matrix components and supplemented at day 14 with the Wnt pathway agonist R-spondin 2 and the bone morphogenic protein antagonist Noggin.

[PLoS One] Researchers presented novel biocompatible Polydimethylsiloxane-based micromechanical tweezers capable of the stiffness characterization and manipulation of hydrogel-based organoids.

[Advanced Science] Investigators established a long-term culture of human fetal thyroid organoids system, which retained the fetal thyroid lineages and molecular signatures, as well as the ability to generate functional human thyroid follicles post mice renal transplantation.