Organoid-Based Modeling of Intestinal Development, Regeneration, and Repair

Scientists discuss how Wnt and YAP signaling pathways control the intestinal repair response and the transitioning of cell states, in comparison with the process of intestinal development.
[Cell Death & Differentiation]
Sprangers, J., Zaalberg, I. C., & Maurice, M. M. (2020). Organoid-based modeling of intestinal development, regeneration, and repair. Cell Death & Differentiation, 1–13. https://doi.org/10.1038/s41418-020-00665-z Cite
Full Article
Bookmark

No account yet? Register

0
Share

A Human Tissue Screen Identifies a Regulator of ER Secretion as a Brain-Size Determinant

Scientists characterized IER3IP1, which regulates the endoplasmic reticulum (ER) function and ECM protein secretion crucial for tissue integrity, the dysregulation of which results in microcephaly.
[Science]
Esk, C., Lindenhofer, D., Haendeler, S., Wester, R. A., Pflug, F., Schroeder, B., Bagley, J. A., Elling, U., Zuber, J., Haeseler, A. von, & Knoblich, J. A. (2020). A human tissue screen identifies a regulator of ER secretion as a brain-size determinant. Science, 370(6519), 935–941. https://doi.org/10.1126/science.abb5390 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men

The authors established a screening strategy to identify drugs that reduce angiotensin converting enzyme 2 levels in human ESC-derived cardiac cells and lung organoids.
[Cell Stem Cell]
Samuel, R. M., Majd, H., Richter, M. N., Ghazizadeh, Z., Zekavat, S. M., Navickas, A., Ramirez, J. T., Asgharian, H., Simoneau, C. R., Bonser, L. R., Koh, K. D., Garcia-Knight, M., Tassetto, M., Sunshine, S., Farahvashi, S., Kalantari, A., Liu, W., Andino, R., Zhao, H., … Fattahi, F. (2020). Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men. Cell Stem Cell, 0(0). https://doi.org/10.1016/j.stem.2020.11.009 Cite
Full ArticleGraphical Abstract
Bookmark

No account yet? Register

0
Share

Cancer-Initiating Cells in Human Pancreatic Cancer Organoids Are Maintained by Interactions with Endothelial Cells

Organoid-derived CD44(−) cancer cells were capable of organoid re-formation and could be re-programed as CD44-expressing cancer-initiating cells in the organoid culture system.
[Cancer Letters]
Choi, J.-I., Jang, S. I., Hong, J., Kim, C. H., Kwon, S. S., Park, J. S., & Lim, J.-B. (2020). Cancer-initiating cells in human pancreatic cancer organoids are maintained by interactions with endothelial cells. Cancer Letters. https://doi.org/10.1016/j.canlet.2020.10.012 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Human Coronavirus Dependency on Host Heat Shock Protein 90 Reveals an Antiviral Target

An Hsp90 inhibitor, 17-AAG, significantly suppressed MERS-CoV propagation in cell lines and physiological-relevant human intestinal organoids.
[Emerging Microbes & Infections]
Li, C., Chu, H., Liu, X., Chiu, M. C., Zhao, X., Wang, D., Wei, Y., Hou, Y., Shuai, H., Cai, J., Chan, J. F.-W., Zhou, J., & Yuen, K. Y. (2020). Human coronavirus dependency on host heat shock protein 90 reveals an antiviral target. Emerging Microbes & Infections, 0(ja), 1–27. https://doi.org/10.1080/22221751.2020.1850183 Cite
Abstract
Bookmark

No account yet? Register

0
Share

The Lumen of Human Intestinal Organoids Poses Greater Stress to Bacteria Compared to the Germ-Free Mouse Intestine: Escherichia coli Deficient in RpoS as a Colonization Probe

Researchers generated an isogenic mutant in the general stress response sigma factor RpoS and employed this mutant to compare challenges faced by a bacterium during colonization of the human intestinal organoids lumen relative to the germ-free mouse intestine.
[mSphere]
Barron, M. R., Cieza, R. J., Hill, D. R., Huang, S., Yadagiri, V. K., Spence, J. R., & Young, V. B. (2020). The Lumen of Human Intestinal Organoids Poses Greater Stress to Bacteria Compared to the Germ-Free Mouse Intestine: Escherichia coli Deficient in RpoS as a Colonization Probe. MSphere, 5(6). https://doi.org/10.1128/mSphere.00777-20 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Formation of Osteochondral Organoids from Murine Induced Pluripotent Stem Cells

To create a model system of these cartilage-bone interactions, researchers developed an osteochondral organoid from murine induced pluripotent stem cells. They grew organoids from a single cell type through time-dependent sequential exposure of growth factors to mirror bone development through endochondral ossification.
[Tissue Engineering]
O’Connor, S., Katz, D., Oswald, S., Groneck, L., & Guilak, F. (2020). Formation of Osteochondral Organoids from Murine Induced Pluripotent Stem Cells. Tissue Engineering Part A. https://doi.org/10.1089/ten.TEA.2020.0273 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Rethinking Organoid Technology through Bioengineering

The authors underline the need for providing engineering solutions to gain control of self-organization and functionality of human PSC-derived organoids.
[Nature Materials]
Garreta, E., Kamm, R. D., Chuva de Sousa Lopes, S. M., Lancaster, M. A., Weiss, R., Trepat, X., Hyun, I., & Montserrat, N. (2020). Rethinking organoid technology through bioengineering. Nature Materials, 1–11. https://doi.org/10.1038/s41563-020-00804-4 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Humanized COVID-19 Decoy Antibody Effectively Blocks Viral Entry and Prevents SARS-CoV-2 Infection

Researchers demonstrated that ACE2‐Fc could specifically abrogate virus replication by blocking the entry of SARS‐CoV‐2 spike‐expressing pseudotyped virus into both ACE2‐expressing lung cells and lung organoids.
[EMBO Molecular Medicine]
Huang, K.-Y., Lin, M.-S., Kuo, T.-C., Chen, C.-L., Lin, C.-C., Chou, Y.-C., Chao, T.-L., Pang, Y.-H., Kao, H.-C., Huang, R.-S., Lin, S., Chang, S.-Y., & Yang, P.-C. (2020). Humanized COVID-19 decoy antibody effectively blocks viral entry and prevents SARS-CoV-2 infection. EMBO Molecular Medicine, n/a(n/a), e12828. https://doi.org/10.15252/emmm.202012828 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Human Soluble ACE2 Improves the Effect of Remdesivir in SARS-CoV-2 Infection

Scientists investigated additive effect of combination therapy using remdesivir with recombinant soluble ACE2 on Vero E6 and kidney organoids, targeting two different modalities of SARS‐CoV‐2 life cycle: cell entry via its receptor ACE2 and intracellular viral RNA replication.
[EMBO Molecular Medicine]
Monteil, V., Dyczynski, M., Lauschke, V. M., Kwon, H., Wirnsberger, G., Youhanna, S., Zhang, H., S.Slutsky, A., Hurtado del Pozo, C., Horn, M., Montserrat, N., Penninger, J. M., & Mirazimi, A. (2020). Human soluble ACE2 improves the effect of remdesivir in SARS-CoV-2 infection. EMBO Molecular Medicine, n/a(n/a), e13426. https://doi.org/10.15252/emmm.202013426 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Reduced Astrocytic Reactivity in Human Brains and Midbrain Organoids with PRKN Mutations

Scientists revealed reduced reactivity of the astrocytic glial fibrillary acidic protein in the degenerated substantia nigra of PRKN-mutated patients, as well as in midbrain organoids generated from iPSCs reprogramed from PRKN-mutated patient fibroblasts.
[npj Parkinson’s Disease]
Kano, M., Takanashi, M., Oyama, G., Yoritaka, A., Hatano, T., Shiba-Fukushima, K., Nagai, M., Nishiyama, K., Hasegawa, K., Inoshita, T., Ishikawa, K., Akamatsu, W., Imai, Y., Bolognin, S., Schwamborn, J. C., & Hattori, N. (2020). Reduced astrocytic reactivity in human brains and midbrain organoids with PRKN mutations. Npj Parkinson’s Disease, 6(1), 1–9. https://doi.org/10.1038/s41531-020-00137-8 Cite
Full Article
Bookmark

No account yet? Register

0
Share
Share