Researchers investigated the effect and mechanisms of corticosteroid prednisolone on epithelial barrier using Crohn’s disease (CD) patient-derived intestinal organoids. 3D intestinal organoids were generated from colon biopsies of inactive CD patients.
[Journal of Molecular Medicine]
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There is an urgent need for robust and practical in vitro model systems to investigate viral pathogenesis. The authors generated human iPSCs-derived lung organoids, cerebral organoids, neural progenitor cells, neurons and astrocytes.
[Stem Cell Reports]
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To investigate the functional importance of an amino acid change in humans, investigators reintroduced the archaic allele into human induced pluripotent cells using genome editing and then followed their neural development through cortical organoids.
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Trujillo, C. A., Rice, E. S., Schaefer, N. K., Chaim, I. A., Wheeler, E. C., Madrigal, A. A., Buchanan, J., Preissl, S., Wang, A., Negraes, P. D., Szeto, R. A., Herai, R. H., Huseynov, A., Ferraz, M. S. A., Borges, F. S., Kihara, A. H., Byrne, A., Marin, M., Vollmers, C., … Muotri, A. R. (2021). Reintroduction of the archaic variant of NOVA1 in cortical organoids alters neurodevelopment. Science, 371(6530). https://doi.org/10.1126/science.aax2537 Cite
Scientists discuss the constraints of matrigel and describe other alternative scaffolds that have been used for organoid cultures.
Researchers investigatef whether Cisplatin, Imatinib, and 5-Fluorouracil alter the tumoroid growth of metastatic colorectal cancer.
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Using primary epithelial organoids from human colon, researchers explored how the central antiviral mediator IFN‐γ, which is elevated in COVID‐19, affected epithelial cell differentiation, ACE2 expression, and susceptibility to infection with SARS‐CoV‐2.
[EMBO Molecular Medicine]
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Heuberger, J., Trimpert, J., Vladimirova, D., Goosmann, C., Lin, M., Schmuck, R., Mollenkopf, H.-J., Brinkmann, V., Tacke, F., Osterrieder, N., & Sigal, M. (2021). Epithelial response to IFN-γ promotes SARS-CoV-2 infection. EMBO Molecular Medicine, n/a(n/a), e13191. https://doi.org/10.15252/emmm.202013191 Cite
A robust organoid engineering approach, Multi‐Organoid Patterning and Fusion was presented to assemble individual airway organoids of different sizes into upscaled, scaffold‐free airway tubes with predefined shapes.
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Liu, Y., Dabrowska, C., Mavousian, A., Strauss, B., Meng, F., Mazzaglia, C., Ouaras, K., Macintosh, C., Terentjev, E., Lee, J.-H., & Huang, Y. Y. S. (n.d.). Bio-assembling Macro-Scale, Lumenized Airway Tubes of Defined Shape via Multi-Organoid Patterning and Fusion. Advanced Science, n/a(n/a), 2003332. https://doi.org/https://doi.org/10.1002/advs.202003332 Cite
Scientists review the current progress in the development of scaffold-based testis organoids while focusing on the scaffolding biomaterials, cell sources, and scaffolding approaches.
[Biology of Reproduction]
The authors provide an overview of the gaps to broader implementation of neuronal organoids in a drug discovery setting, as well as emerging technologies that may better enable their utilization.
Scientists outline the main challenges and possible risks regarding the potential translation of gastrointestinal organoids from laboratory settings to patient-specific models in clinical applications.
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The discovery of stem cells has offered a new source of cells, while their use in generating gastrointestinal and brain organoids has enabled the development of novel 3D tissues that better mimic the native tissue structure and function, compared with traditional assays. Scientists discuss how recent advances and trends in this area can be applied in host–microbe and host–pathogen interaction studies.
[Biochemical Society Transactions]