Corticosteroid Enhances Epithelial Barrier Function in Intestinal Organoids Derived from Patients with Crohn’s Disease

Researchers investigated the effect and mechanisms of corticosteroid prednisolone on epithelial barrier using Crohn’s disease (CD) patient-derived intestinal organoids. 3D intestinal organoids were generated from colon biopsies of inactive CD patients.
[Journal of Molecular Medicine]
Full Article
Bookmark

No account yet? Register

0
Share

Revealing Tissue-Specific SARS-CoV-2 Infection and Host Responses Using Human Stem Cell Derived Lung and Cerebral Organoids

There is an urgent need for robust and practical in vitro model systems to investigate viral pathogenesis. The authors generated human iPSCs-derived lung organoids, cerebral organoids, neural progenitor cells, neurons and astrocytes.
[Stem Cell Reports]
Tiwari, S. K., Wang, S., Smith, D., Carlin, A., & Rana, T. M. (2021). Revealing tissue-specific SARS-CoV-2 infection and host responses using human stem cell derived lung and cerebral organoids. Stem Cell Reports, 0(0). https://doi.org/10.1016/j.stemcr.2021.02.005 Cite
AbstractFull ArticleGraphical Abstract
Bookmark

No account yet? Register

0
Share

Reintroduction of the Archaic Variant of NOVA1 in Cortical Organoids Alters Neurodevelopment

To investigate the functional importance of an amino acid change in humans, investigators reintroduced the archaic allele into human induced pluripotent cells using genome editing and then followed their neural development through cortical organoids.
[Science]
Trujillo, C. A., Rice, E. S., Schaefer, N. K., Chaim, I. A., Wheeler, E. C., Madrigal, A. A., Buchanan, J., Preissl, S., Wang, A., Negraes, P. D., Szeto, R. A., Herai, R. H., Huseynov, A., Ferraz, M. S. A., Borges, F. S., Kihara, A. H., Byrne, A., Marin, M., Vollmers, C., … Muotri, A. R. (2021). Reintroduction of the archaic variant of NOVA1 in cortical organoids alters neurodevelopment. Science, 371(6530). https://doi.org/10.1126/science.aax2537 Cite
AbstractPress ReleaseGraphical Abstract
Bookmark

No account yet? Register

0
Share

Non-Matrigel Scaffolds for Organoid Cultures

Scientists discuss the constraints of matrigel and describe other alternative scaffolds that have been used for organoid cultures.
[Cancer Letters]
Kaur, S., Kaur, I., Rawal, P., Tripathi, D. M., & Vasudevan, A. (2021). Non-matrigel scaffolds for organoid cultures. Cancer Letters. https://doi.org/10.1016/j.canlet.2021.01.025 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Gel-Free 3D Tumoroids with Stem Cell Properties Modeling Drug Resistance to Cisplatin and Imatinib in Metastatic Colorectal Cancer

Researchers investigatef whether Cisplatin, Imatinib, and 5-Fluorouracil alter the tumoroid growth of metastatic colorectal cancer.
[Cells]
Sogawa, C., Eguchi, T., Namba, Y., Okusha, Y., Aoyama, E., Ohyama, K., & Okamoto, K. (2021). Gel-Free 3D Tumoroids with Stem Cell Properties Modeling Drug Resistance to Cisplatin and Imatinib in Metastatic Colorectal Cancer. Cells, 10(2), 344. https://doi.org/10.3390/cells10020344 Cite
Full Article
Bookmark

No account yet? Register

0
Share

Epithelial Response to IFN-γ Promotes SARS-CoV-2 Infection

Using primary epithelial organoids from human colon, researchers explored how the central antiviral mediator IFN‐γ, which is elevated in COVID‐19, affected epithelial cell differentiation, ACE2 expression, and susceptibility to infection with SARS‐CoV‐2.
[EMBO Molecular Medicine]
Heuberger, J., Trimpert, J., Vladimirova, D., Goosmann, C., Lin, M., Schmuck, R., Mollenkopf, H.-J., Brinkmann, V., Tacke, F., Osterrieder, N., & Sigal, M. (2021). Epithelial response to IFN-γ promotes SARS-CoV-2 infection. EMBO Molecular Medicine, n/a(n/a), e13191. https://doi.org/10.15252/emmm.202013191 Cite
AbstractFull Article
Bookmark

No account yet? Register

0
Share

Bio‐assembling Macro‐Scale, Lumenized Airway Tubes of Defined Shape via Multi‐Organoid Patterning and Fusion

A robust organoid engineering approach, Multi‐Organoid Patterning and Fusion was presented to assemble individual airway organoids of different sizes into upscaled, scaffold‐free airway tubes with predefined shapes.
[Advanced Science]
Liu, Y., Dabrowska, C., Mavousian, A., Strauss, B., Meng, F., Mazzaglia, C., Ouaras, K., Macintosh, C., Terentjev, E., Lee, J.-H., & Huang, Y. Y. S. (n.d.). Bio-assembling Macro-Scale, Lumenized Airway Tubes of Defined Shape via Multi-Organoid Patterning and Fusion. Advanced Science, n/a(n/a), 2003332. https://doi.org/https://doi.org/10.1002/advs.202003332 Cite
Full ArticlePress Release
Bookmark

No account yet? Register

0
Share

Current Progress, Challenges, and Future Prospects of Testis Organoids

Scientists review the current progress in the development of scaffold-based testis organoids while focusing on the scaffolding biomaterials, cell sources, and scaffolding approaches.
[Biology of Reproduction]
Cham, T.-C., Chen, X., & Honaramooz, A. (2021). Current Progress, Challenges, and Future Prospects of Testis Organoids. Biology of Reproduction, ioab014. https://doi.org/10.1093/biolre/ioab014 Cite
Abstract
Bookmark

No account yet? Register

0
Share

The Emerging Role of Neuronal Organoid Models in Drug Discovery: Potential Applications and Hurdles to Implementation

The authors provide an overview of the gaps to broader implementation of neuronal organoids in a drug discovery setting, as well as emerging technologies that may better enable their utilization.
[Molecular Pharmacology]
Struzyna, L. A., & Watt, M. L. (2021). The Emerging Role of Neuronal Organoid Models in Drug Discovery: Potential Applications and Hurdles to Implementation. Molecular Pharmacology. https://doi.org/10.1124/molpharm.120.000142 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Emerging Biofabrication Approaches for Gastrointestinal Organoids towards Patient Specific Cancer Models

Scientists outline the main challenges and possible risks regarding the potential translation of gastrointestinal organoids from laboratory settings to patient-specific models in clinical applications.
[Cancer Letters]
Soto, F., Guimarães, C. F., Reis, R. L., Franco, W., Rizvi, I., & Demirci, U. (2021). Emerging biofabrication approaches for gastrointestinal organoids towards patient specific cancer models. Cancer Letters. https://doi.org/10.1016/j.canlet.2021.01.023 Cite
Abstract
Bookmark

No account yet? Register

0
Share

Advances in Modeling the Human Microbiome–Gut–Brain Axis In Vitro

The discovery of stem cells has offered a new source of cells, while their use in generating gastrointestinal and brain organoids has enabled the development of novel 3D tissues that better mimic the native tissue structure and function, compared with traditional assays. Scientists discuss how recent advances and trends in this area can be applied in host–microbe and host–pathogen interaction studies.
[Biochemical Society Transactions]
Moysidou, C.-M., & Owens, R. M. (2021). Advances in modelling the human microbiome–gut–brain axis in vitro. Biochemical Society Transactions, BST20200338. https://doi.org/10.1042/BST20200338 Cite
Full Article
Bookmark

No account yet? Register

0
Share
Share