An Organoid-Based Drug Screening Identified a Menin-MLL Inhibitor for Endometrial Cancer through Regulating the HIF Pathway

CRISPR/cas9-mediated mutations of major components of the menin-MLL complex, Men1, Kmt2a and Ash2l, inhibited the growth of tumor organoids, suggesting that the complex was the target of MI-136.
[Cancer Gene Therapy]
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Probiotic Properties of Escherichia coli Nissle in Human Intestinal Organoids

To assess the safety of and protection conferred by Nissle, researchers modeled infection in stem cell-derived human intestinal organoids.
[American Society for Microbiology]
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Cancer Cells Educate Natural Killer Cells to a Metastasis-Promoting Cell State

Using ex vivo and in vivo models of metastasis, scientists established that keratin-14+ breast cancer cells were vulnerable to natural killer cells.
[Journal of Cell Biology]
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Human Organoids: Model Systems for Human Biology and Medicine

The authors discuss the applications, advantages and disadvantages of human organoids as models of development and disease and outlines the challenges that have to be overcome for organoids to be able to substantially reduce the need for animal experiments.
[Nature Reviews Molecular Cell Biology]
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Loss of Function of the Mitochondrial Peptidase PITRM1 Induces Proteotoxic Stress and Alzheimer’s Disease-Like Pathology in Human Cerebral Organoids

To investigate the pathogenetic mechanisms of mitochondrial presequence processing, researchers employed cortical neurons and cerebral organoids generated from PITRM1-knockout human iPSCs.
[Molecular Psychiatry]
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TIGAR/AP-1 Axis Accelerates the Division of Lgr5 Reserve Intestinal Stem Cells to Reestablish Intestinal Architecture after Lethal Radiation

Scientists demonstrated that post-ionizing radiation (IR) overexpression of TIGAR, a critical downstream target of c-MYC in mouse intestine, mounted a hyperplastic response in Bmi1-creERT+ reserve intestinal stem cells, and thus rescued mice from lethal IR exposure.
[Cell Death & Disease]
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Characterizing Caspase-1 Involvement during Esophageal Disease Progression

Barrett’s esophagus patient biopsies and murine Barrett’s esophagus organoids were cultured ex vivo in the presence of a caspase-1 inhibitor, to determine the importance of caspase-1 for inflammatory cytokine and chemokine secretion.
[Cancer Immunology Immunotherapy]
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Long Term Exposure to a Grape Seed Proanthocyanidin Extract Enhances L‐Cell Differentiation in Intestinal Organoids

Mice ileum organoids were used to evaluate the long‐term effects of grape‐seed proanthocyanidin extract and two of its main components, epicatechin and gallic acid, on intestinal differentiation.
[Molecular Nutrition & Food Research]
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Quiescent Stem Cell Marker Genes in Glioma Gene Networks are Sufficient to Distinguish between Normal and Glioblastoma (GBM) Samples

Scientists demonstrated that GBM associated gene regulatory modules were characterized by diagnostic quiescent stem cell marker genes.
[Scientific Reports]
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Congruence of Transcription Programs in Adult Stem Cell-Derived Jejunum Organoids and Original Tissue during Long-Term Culture

Researchers investigated the transcriptomes of jejunum organoids derived from the same pig as well as batch-to-batch variation of organoids derived from different pigs over long-term passage.
[Frontiers in Cell and Developmental Biology]
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Implementing Cell-Free DNA of Pancreatic Cancer Patient-Derived Organoids for Personalized Oncology

Investigators tested conditioned media of individual patient-derived organoids (PDOs) for cell-free tumor DNA to detect driver mutations already early on during the expansion process in order to accelerate the genetic characterization of PDOs as well as subsequent functional testing.
[JCI Insight]
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