organoids

[International Journal of Molecular Sciences] The authors carried out high-throughput functional screening using impedancemetry, and identified five miRNAs with potential antiproliferative or chemosensitive effects on SW1353 chondrosarcoma cells.

[Cancers] Three paired pancreatic ductal adenocarcinoma preclinical models-patient-derived xenografts , xenograft-derived pancreatic organoids and xenograft-derived primary cell cultures-were derived from 20 patients and analyzed at the transcriptomic and chemosensitivity level.

[Acta Neuropathologica Communications] Investigators interrogated gene expression in separate microenvironments of glioblastoma organoid models that mimicked the transition between nutrient-rich and nutrient-poor pseudopalisading/perinecrotic tumor zones using spatial-capture RNA-sequencing.

[Science Advances] Researchers developed a reproducible two-step protocol to induce human pluripotent stem cells to differentiate into highly expandable human intestinal epithelial cells (hIECs) progenitors and a functional hIEC monolayer exhibiting intestinal molecular features, cell type diversity, and high activities of intestinal transporters and metabolic enzymes such as cytochrome P450 3A4.

[Journal of Experimental & Clinical Cancer Research] Scientists highlight the advantages and limits of patient-derived models as innovative and valuable pre-clinical tools to study progression and metastasis of colorectal cancer, develop novel therapeutic strategies by creating a drug screening platform, and predict the efficacy of clinical response to therapy.

[Marine Drugs] The impact of surface charge on the delivery of 5-ASA loaded PLGA nanoparticles into the lumen of organoids was investigated. Alginate and chitosan were used to coat the nanoparticles and provide negative and positive charges on the particles, respectively.