Investigators evaluated the osteogenic capability of placental stem cell-derived extracellular vesicles (EVs) and compared them to the well-characterized bone marrow MSC-derived EVs. EVs were extracted from three designated time points after osteogenic differentiation.
The constructed multiplex siRNAs effectively knocked down the expression of Smad4 and/or Smad1, Smad5, and Smad8 in MSCs, and inhibited all aspects of BMP9-induced osteogenic differentiation in bone marrow MSCs, including decreased expression of osteogenic regulators/markers, reduced osteogenic marker ALP activity, and diminished in vitro matrix mineralization and in vivo ectopic bone formation
[Molecular Therapy-Nucleic Acids]
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He, F., Ni, N., Zeng, Z., Wu, D., Feng, Y., Li, A. J., Luu, B., Li, A. F., Qin, K., Wang, E., Wang, X., Wu, X., Luo, H., Zhang, J., Zhang, M., Mao, Y., Pakvasa, M., Wagstaff, W., Zhang, Y., … Fan, J. (2020). FAMSi: A Synthetic Biology Approach to the Fast Assembly of Multiplex siRNAs for Silencing Gene Expression in Mammalian Cells. Molecular Therapy - Nucleic Acids, 0(0). https://doi.org/10.1016/j.omtn.2020.10.007 Cite
The effects of miR‐140‐5p on tissue‐to‐bone healing was investigated. Clone formation experiment, flow cytometry, Alizarin Red S Staining and Oil Red O Staining were performed to investigate the biological characteristics of mouse embryonic bone marrow MSCs C3H10T1/2.
[Cell Biochemistry and Function]
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The authors investigated in vitro cellular networks derived from human gingival mesenchymal stem cells (hGMSCs) and calcium phosphate (CaP) bioceramic interaction.
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Benjumeda Wijnhoven, I., Vallejos, R., Santibanez, J. F., Millán, C., & Vivanco, J. F. (2020). Analysis of cell-biomaterial interaction through cellular bridge formation in the interface between hGMSCs and CaP bioceramics. Scientific Reports, 10(1), 16493. https://doi.org/10.1038/s41598-020-73428-y Cite
Scientists evaluated the effects of αMEM, aDMEM, M199, αMEM/M199, aDMEM/M199 and αMEM/aDMEM mediaon porcine bone marrow MSC derivation, expression of number of osteogenic markers at fifth and tenth passage in multipotentporcine (p)MSCs before differentiation, and differentiation of pMSC to osteogenic lineage.
Using multiple human breast cancer cell lines, the osteogenic cocktail model was established in vitro to demonstrate calcification-exacerbated metastasis.
[British Journal of Cancer]
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Scientists focused on investigating extracorporeal shock waves for their effects on proliferation, migration, and osteogenic differentiation in an injectable gelatin matrix capable of undergoing covalent cross‐linking in vivo.
[Journal of Tissue Engineering and Regenerative Medicine]
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The effects of shock wave stimulation of mesenchymal stem cells on proliferation, migration, and differentiation in an injectable gelatin matrix for osteogenic regeneration - Alshihri - - Journal of Tissue Engineering and Regenerative Medicine - Wiley Online Library. (n.d.). Retrieved September 4, 2020, from https://onlinelibrary.wiley.com/doi/abs/10.1002/term.3126 Cite
Human adipose-derived stromal cells and bone marrow-derived stromal cellswere characterized by FACS analysis and tri-lineage differentiation, followed by an intraindividual comparative proteomic analysis upon osteogenic differentiation.
[Journal of Cellular and Molecular Medicine]
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Dadras, M., May, C., Wagner, J. M., Wallner, C., Becerikli, M., Dittfeld, S., Serschnitzki, B., Schilde, L., Guntermann, A., Sengstock, C., Köller, M., Seybold, D., Geßmann, J., Schildhauer, T. A., Lehnhardt, M., Marcus, K., & Behr, B. (n.d.). Comparative proteomic analysis of osteogenic differentiated human adipose tissue and bone marrow-derived stromal cells. Journal of Cellular and Molecular Medicine, n/a(n/a). https://doi.org/10.1111/jcmm.15797 Cite
Researchers report a combinational peptide screening strategy for rapid discovery of ligands that not only bind strongly to osteogenic progenitor cells (OPCs) but also stimulate osteogenic cell Akt signaling in those OPCs.
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Motivated by the enormous advantages of Zn‐based metal-organic framework‐derived nanocarbons, C‐ZnO nanocarbons‐modified fibrous scaffolds for stem cell‐based osteogenic differentiation were constructed.
Scientists showed that circRNA-vgll3 originating from the vgll3 locus markedly enhanced osteogenic differentiation of adipose-derived mesenchymal stem cells.
[Cell Death & Differentiation]
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Zhang, D., Ni, N., Wang, Y., Tang, Z., Gao, H., Ju, Y., Sun, N., He, X., Gu, P., & Fan, X. (2020). CircRNA-vgll3 promotes osteogenic differentiation of adipose-derived mesenchymal stem cells via modulating miRNA-dependent integrin α5 expression. Cell Death & Differentiation, 1–20. https://doi.org/10.1038/s41418-020-0600-6 Cite