Dual Effects of Hypoxia on Proliferation and Osteogenic Differentiation of Mouse Clonal Mesenchymal Stem Cells

Mouse clonal MSCs were cultured on a Cytodex 3 microcarrier in a spinner flask for a suspension culture under hypoxia condition to increase mass productivity.
[Bioprocess and Biosystems Engineering]
Kim, H., & Kwon, S. (2021). Dual effects of hypoxia on proliferation and osteogenic differentiation of mouse clonal mesenchymal stem cells. Bioprocess and Biosystems Engineering. https://doi.org/10.1007/s00449-021-02563-1 Cite
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Functional Tissue-Engineered Bone-Like Graft Made of a Fibrin Scaffold and TG2 Gene-Modified EMSCs for Bone Defect Repair

A novel biofunctional live tissue-engineered bone-like graft was designed and constructed using a fibrin scaffold loaded with TG2 gene-modified ectomesenchymal stem cells (TG2-EMSCs) derived from nasal respiratory mucosa for bone defect repair.
[NPG Asia Materials]
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Biomimetic Cell-Adhesive Ligand-Functionalized Peptide Composite Hydrogels Maintain Stemness of Human Amniotic Mesenchymal Stem Cells

Scientists investigated the effects of the incorporation of cell-adhesive ligands in 3D self-assembling peptide hydrogels to modulate stem cell survival, proliferation, maintenance of stemness, and osteogenic differentiation.
[Regenerative Biomaterials]
Zhang, L., Xiong, N., Liu, Y., & Gan, L. (2021). Biomimetic cell-adhesive ligand-functionalized peptide composite hydrogels maintain stemness of human amniotic mesenchymal stem cells. Regenerative Biomaterials, 8(rbaa057). https://doi.org/10.1093/rb/rbaa057 Cite
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Pre-Conditioning of Mesenchymal Stem Cells with Piper longum L. Augments Osteogenic Differentiation

Proliferation of Wharton’s jelly MSCs treated with Piper longum L. fruit extract was assessed by MTT assay and Cell Cycle Analysis. Effect of Piper longum L. preconditioning on osteogenic differentiation was performed.
[Journal of Ethnopharmacology]
Sanap, A., Joshi, K., Shah, T., Tillu, G., & Bhonde, R. (2021). Pre-conditioning of Mesenchymal Stem Cells with Piper longum L. augments osteogenic differentiation. Journal of Ethnopharmacology, 113999. https://doi.org/10.1016/j.jep.2021.113999 Cite
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Bone Marrow Stromal Cells from MDS and AML Patients Show Increased Adipogenic Potential with Reduced Delta-Like-1 Expression

Researchers established a stringent flow cytometric protocol for the prospective isolation of a CD73+ CD105+ CD271+ bone marrow (BM) stromal cell subpopulation from uncultivated cryopreserved BM of myelodysplastic syndrome and acute myeloid leukemia patients as well as age-matched healthy donors.
[Scientific Reports]
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Mapping the Immune Microenvironment for Mandibular Alveolar Bone Homeostasis at Single-Cell Resolution

Researchers constructed a single-cell atlas of mouse mandibular alveolar bone through single-cell RNA sequencing. A more active immune microenvironment was identified in alveolar bone, with a higher proportion of mature immune cells than in long bone.
[Bone Research]
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Polo Like Kinase 4 (PLK4) Impairs Human Bone Marrow Mesenchymal Stem Cell (BMSC) Viability and Osteogenic Differentiation

Researchers identified the aberrant PLK4 upregulation in osteoporosis and downregulation in BMSCs during osteogenic differentiation. In isolated human BMSCs (hBMSCs), PLK4 overexpression significantly inhibited, whereas PLK4 knockdown promoted cell viability and hBMSC osteogenic differentiation.
[Biochemical and Biophysical Research Communications]
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Down Regulation of SUV39H1 and CITED2 Exerts Additive Effect on Promoting Adipogenic Commitment of Human Mesenchymal Stem Cells

Through a siRNA-based high throughput screen that identifies adipogenic regulators whose expression knockdown leads to enhanced adipogenic differentiation of human mesenchymal stem cells, two new regulators, SUV39H1, a histone methyltransferase that catalyzes H3K9Me3, and CITED2, a CBP/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 2 were uncovered.
[Stem Cells and Development]
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Improved Healing of Critical-Size Femoral Defect in Osteoporosis Rat Models Using 3D Elastin/Polycaprolactone/nHA Scaffold in Combination with Mesenchymal Stem Cells

Elastin/polycaprolactone/nHA nanofibrous scaffold in combination with mesenchymal stem cells were used to regenerate bone defects. Cytotoxicity, cytocompatibility and cellular morphology were evaluated in vitro and observations revealed that an appropriate environment for cellular attachment, growth, migration, and proliferation is provided by this scaffold.
[Journal of Materials Science-Materials in Medicine]
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Dual Anisotropicity Comprising 3D Printed Structures and Magnetic Nanoparticle Assemblies: Towards the Promotion of Mesenchymal Stem Cell Osteogenic Differentiation

Scientists designed a substrate with a dual anisotropic structure fabricated via a combination of 3D printing and magnetic field-induced magnetic nanoparticle assembly techniques. These dual anisotropic structures had a scale hierarchy, and the scale of the magnetic nanoparticle assemblies matched that of a single adipose tissue-derived stem cells.
[NPG Asia Materials]
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Ubiquitin-Specific Protease 53 Promotes Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

Researchers investigate the role of ubiquitin-specific protease 53 in the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells.
[Cell Death & Disease]
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