Mouse clonal MSCs were cultured on a Cytodex 3 microcarrier in a spinner flask for a suspension culture under hypoxia condition to increase mass productivity.
[Bioprocess and Biosystems Engineering]
A novel biofunctional live tissue-engineered bone-like graft was designed and constructed using a fibrin scaffold loaded with TG2 gene-modified ectomesenchymal stem cells (TG2-EMSCs) derived from nasal respiratory mucosa for bone defect repair.
[NPG Asia Materials]
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Scientists investigated the effects of the incorporation of cell-adhesive ligands in 3D self-assembling peptide hydrogels to modulate stem cell survival, proliferation, maintenance of stemness, and osteogenic differentiation.
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Proliferation of Wharton’s jelly MSCs treated with Piper longum L. fruit extract was assessed by MTT assay and Cell Cycle Analysis. Effect of Piper longum L. preconditioning on osteogenic differentiation was performed.
[Journal of Ethnopharmacology]
Researchers established a stringent flow cytometric protocol for the prospective isolation of a CD73+ CD105+ CD271+ bone marrow (BM) stromal cell subpopulation from uncultivated cryopreserved BM of myelodysplastic syndrome and acute myeloid leukemia patients as well as age-matched healthy donors.
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Researchers constructed a single-cell atlas of mouse mandibular alveolar bone through single-cell RNA sequencing. A more active immune microenvironment was identified in alveolar bone, with a higher proportion of mature immune cells than in long bone.
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Researchers identified the aberrant PLK4 upregulation in osteoporosis and downregulation in BMSCs during osteogenic differentiation. In isolated human BMSCs (hBMSCs), PLK4 overexpression significantly inhibited, whereas PLK4 knockdown promoted cell viability and hBMSC osteogenic differentiation.
[Biochemical and Biophysical Research Communications]
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Through a siRNA-based high throughput screen that identifies adipogenic regulators whose expression knockdown leads to enhanced adipogenic differentiation of human mesenchymal stem cells, two new regulators, SUV39H1, a histone methyltransferase that catalyzes H3K9Me3, and CITED2, a CBP/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 2 were uncovered.
[Stem Cells and Development]
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Elastin/polycaprolactone/nHA nanofibrous scaffold in combination with mesenchymal stem cells were used to regenerate bone defects. Cytotoxicity, cytocompatibility and cellular morphology were evaluated in vitro and observations revealed that an appropriate environment for cellular attachment, growth, migration, and proliferation is provided by this scaffold.
[Journal of Materials Science-Materials in Medicine]
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Scientists designed a substrate with a dual anisotropic structure fabricated via a combination of 3D printing and magnetic field-induced magnetic nanoparticle assembly techniques. These dual anisotropic structures had a scale hierarchy, and the scale of the magnetic nanoparticle assemblies matched that of a single adipose tissue-derived stem cells.
[NPG Asia Materials]
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Researchers investigate the role of ubiquitin-specific protease 53 in the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells.
[Cell Death & Disease]
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