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qRT-PCR

MiR-135b-5p Is an Oncogene in Pancreatic Cancer to Regulate GPRC5A Expression by Targeting Transcription Factor KLF4

[Cell Death Discovery] Scientists probed molecular regulatory mechanism of KLF4 in malignant progression of pancreatic cancer.

Targeting of Noncoding RNAs Encoded by a Novel MYC Enhancers Inhibits the Proliferation of Human Hepatic Carcinoma Cells In Vitro

[Scientific Reports] By analyzing H3K27ac enrichment and enhancer RNA expression in cultured hepatocellular carcinoma cells, scientists identified six putative MYC enhancer regions.

Human Amniotic Mesenchymal Stem Cells Combined with PPCNg Facilitate Injured Endometrial Regeneration

[Stem Cell Research & Therapy] The authors investigated the therapeutic effect of human amniotic MSCs combined with polyethylene glycol citrate-co-N-isopropylacrylamide mixed with gelatin (PPCNg) transplantation in promoting the regeneration of injured endometrium.

Effect of Lentivirus-Mediated Growth and Differentiation Factor-5 Transfection on Differentiation of Rabbit Nucleus Pulposus Mesenchymal Stem Cells

[European Journal of Medical Research] Researchers investigated the biological and cytological characteristics of rabbit nucleus pulposus MSCs (NPMSCs) and determined the effect of the growth and differentiation factor-5 on the differentiation of rabbit NPMSCs transduced with a lentivirus vector.

The Circ-PITX1 Promotes Non-small Cell Lung Cancer Development via the miR-30e-5p/ITGA6 Axis

[Cell Cycle] Gain- and loss- of function assay verified the impact of circ-PITX1 and miR-30e-5p on the proliferation, invasion, and migration of NSCLC cells.

A Novel circ_MACF1/miR-942-5p/TGFBR2 Axis Regulates the Functional Behaviors and Drug Sensitivity in Gefitinib-Resistant Non-Small Cell Lung Cancer Cells

[BMC Pulmonary Medicine] Scientists established gefitinib-resistant NSCLC cells. The levels of circ_MACF1, microRNA-942-5p, and transforming growth factor beta receptor 2 were gauged by quantitative real-time PCR or western blot.

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