qRT-PCR

[Molecular Cancer] The authors investigated the expression profile of circular RNAs in breast cancer MCF-7 cells under hypoxia and normoxia using microarray.

[Acta Diabetologica] CCK8, Ki67 staining, immunohistochemical analyses, glucose-stimulated insulin secretion and intraperitoneal glucose tolerance test were performed to detect the effect of Kcnq1ot1 on β-cell proliferation and insulin secretion in vitro and in vivo.

[Hepatology] Researchers performed single-cell RNA-sequencing using AKT/NICD-induced mouse intrahepatic cholangiocarcinoma tissues at early, middle, and late stages.

[Prostate] Through co-targeting the androgen receptor (AR) signaling, Diptoindonesin G robustly improved the efficacy of HSP90 inhibitors and enzalutamide in both human prostate cancer cells and in vivo xenograft mouse model.

[Biomedical Materials] Ti3C2 MXene was incorporated into composite scaffolds composed of hydroxyapatite and sodium alginate via extrusion-based 3D printing to evaluate its potential in bone regeneration.

[British Journal of Cancer] Scientists demonstrated that lysine acetyltransferase 6 A (KAT6A) expression was upregulated in HCC tissues and cell lines. Clinical analysis showed that increased KAT6A was significantly associated with malignant prognostic features and shorter survival.