reporter assay

[Biochemical Genetics] Circular RNA-trefoil factor 1 (circ-TFF1) and interleukin-1 receptor-associated kinase 1 (IRAK1) were significantly high expressed in breast cancer tissues and cells. Silencing of circ-TFF1 reduced the proliferation, migration, invasion and tube formation, while increased the apoptosis of MDA-MB-361 and SK-Br-3 cells.

[Experimental Cell Research] Scientists studied the effects of miR-127-3p-enriched extracellular vesicles on choriocarcinoma and underlying mechanisms.

[Cellular & Molecular Biology Letters] The authors suggested that miR-5195-3p functioned as a tumor suppressor by targeting Cyclin L1 (CCNL1) in prostate cancer.

[European Journal of Histochemistry] Researchers showed that the level of miR-19a was significantly higher in clinical CRC tissue samples than in paracancerous tissue samples, and significantly higher in CRC cells lines HT29, SW480, and CaCO2 than in the normal human colon mucosal epithelial cell line NCM460.

[Cell Cycle] A biotin-microRNA pull-down assay was performed to identify potential miRNAs. qRT-PCR was used to verify the KRT18 and miR-186-3p levels.

[Cell Death Discovery] Scientists analyzed the regulatory relationship of hypoxia and PVT1 and the mechanism of PVT1 in the hypoxia-induced chemoresistance process of lung cancer.